Summary Recombinant DNA Notes

-Genetic_ Engineering

Direct
·

manipulation of
genes
Transgene
·

is put into other
gene organism
Recombinent
·

to GMO
DNA
technology moves
genes create a




·
Recombinent _
DNA-



·

Uses manipulated bacteria (E Coli) .




Quick to
-




reproduce

Easy to
-



culture


Has plasmid bacteria
·




plasmid accepted by all




#




·

Plasmid is removed and cut restriction enzyme

Human
·

insulin
gene
:




Taken from
-




Pancreas


Removed cut
-


and




Insulin bacteria plasmid
·

added to
gene
DNA fuses plasmid
ligase
·

and human
gene
Makes recombinent plasmid

Not
·

all plasmids take gene! up (non-recombinent clasmid)

resistant "trailer
3)

Anti-biotic
·

added to
gene gene
Some bacteria plasmids already anti-biotic resistant !




·
Recombinent + non-recombinent plasmid mixed with E . Coli


Introduces plasmid to host
-




cells


Only
·

some E Coli pick plasmids (R + non-R)
.
up


These are transformed bacteria



Bacteria
·

to
agar plate
added with ampicilin

Only transformed bacteria
·

survives!


Anti-biotics bacteria
-




added to


Only bacteria with human + trailer
gene survive




Bacteria
·

with human placed in fermentation tank
gene

-Multiplies
-



Produces insulin