Phlebotomy Certification Comprehensive Exam
Study Guide 2025 | Pass Guaranteed | Verified
Content
Capillary Puncture - 3rd or 4th finger for adults, heel stick for infants; Lancet cut across
ridges; hold the tube horizontal when filling. Don't sqeeze finger too tightly or milk the finger.
Wipe off 1st drop.
The vein can collapse - If you pull on the plunger of the syringe too hard or too quickly
during a draw or you use too strong of a vaccum for the vein, this can happen...
Anticoagulant - An agent used to preven blood clotting and to keep the specimen whole
blood
Red top - NO ADDITIVE --- Clot Forms ----Serum used for Chemistries, Electrolytes,
Thyroids (TSH, T4), Hormone, Liver, Kidney, Lipid stuidies, CMP, BMP.
Tiger top - Aka Red/Gray top: Clot activator additive - Clot Forms - SERUM STUDIES
Lavender top - EDTA additive - No Clot - WHOLE BLOOD for: Complete blood count (CBC),
Blood smear, Blood banking (ABO/Rh), ESR, HCT, Hmg,Glyco Alc ****Must be filled sufficiently
and inverted (gentle rocked) several times****
Gold Top - Clot activator additive - Clot Forms - SERUM Studies
Grey top - Sodium Fluoride/Potassium oxalate - Glucose, Alcohol, FBS
Light blue top - Sodium citrate additive - No Clot - Used for coagulation studies (PT, PTT)
****Tube must be filled to the proper level and inverted (gentle rocked) several times****
Wipe away the first drop - We do this to remove tissue juices, skin cells, alcohol,
contaminates, avoid activating clotting process, avoid producing incorrect results, avoid diluting
specimen.
The basilic, cephilic, and the median - What veins are in the antecubical area?
Hemoconcentration - This is the increase of the cellular/solid elements in direct ratio to
plasma This can be caused by "pumping of the fist" and begins after the tourniquet is left on
longer than 1 minute.
Provodine/iodine - Agent used on the skin to sterilize the area.
Study Guide 2025 | Pass Guaranteed | Verified
Content
Capillary Puncture - 3rd or 4th finger for adults, heel stick for infants; Lancet cut across
ridges; hold the tube horizontal when filling. Don't sqeeze finger too tightly or milk the finger.
Wipe off 1st drop.
The vein can collapse - If you pull on the plunger of the syringe too hard or too quickly
during a draw or you use too strong of a vaccum for the vein, this can happen...
Anticoagulant - An agent used to preven blood clotting and to keep the specimen whole
blood
Red top - NO ADDITIVE --- Clot Forms ----Serum used for Chemistries, Electrolytes,
Thyroids (TSH, T4), Hormone, Liver, Kidney, Lipid stuidies, CMP, BMP.
Tiger top - Aka Red/Gray top: Clot activator additive - Clot Forms - SERUM STUDIES
Lavender top - EDTA additive - No Clot - WHOLE BLOOD for: Complete blood count (CBC),
Blood smear, Blood banking (ABO/Rh), ESR, HCT, Hmg,Glyco Alc ****Must be filled sufficiently
and inverted (gentle rocked) several times****
Gold Top - Clot activator additive - Clot Forms - SERUM Studies
Grey top - Sodium Fluoride/Potassium oxalate - Glucose, Alcohol, FBS
Light blue top - Sodium citrate additive - No Clot - Used for coagulation studies (PT, PTT)
****Tube must be filled to the proper level and inverted (gentle rocked) several times****
Wipe away the first drop - We do this to remove tissue juices, skin cells, alcohol,
contaminates, avoid activating clotting process, avoid producing incorrect results, avoid diluting
specimen.
The basilic, cephilic, and the median - What veins are in the antecubical area?
Hemoconcentration - This is the increase of the cellular/solid elements in direct ratio to
plasma This can be caused by "pumping of the fist" and begins after the tourniquet is left on
longer than 1 minute.
Provodine/iodine - Agent used on the skin to sterilize the area.
