Phlebotomy Nursing Mastery 2025/26 | Latest
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Random Urine Specimens - collected for a urinalysis.
Midstream Specimen - is also called a clean-voided specimen or a clean-catch specimen
The 24-hour specimen - is collected during a 24-hour period. Urine is typically chilled or
refrigerated to prevent the growth of microbes.
Testing for ph - measures if urine is acidic or alkaline
Testing for glucose or ketones - Typically used for testing for diabetes
Testing for blood - injuries or illnesses can cause blood to appear in the urine-
(hematuria)
Testing for blood - Blood can also appear in stools. Ulcers, some forms of cancer, or
hemorrhoids
Culture & Sensitivity - cultivating a specimen in order to determine whether the
developed organism is sensitive to antibiotics
Throat Culture - Throat cultures are used to determine strep throat, whooping cough and
epiglottitis
Wound Culture - Specimens may be collected from animal bites, ulcers, and skin wounds
Urinalysis - is usually conducted on patient's that are pregnant, have an illness, or are
suspected to have abused an illegal substance.
Urine- collection in temp measured cups - Check for clarity, specific gravity, color and
odor
Cerebrospinal Fluid - mostly obtained through a lumbar puncture and looks for cell
counts, chloride, glucose, and total __________
2- hour Post prandial Glucose - Glucose specimen is collected 2 hours after a patient eats
a meal
Glucose Tolerance Test - must fast at least 12 hours before the test. Patients must drink
glucose prior to testing and have 5 minutes to finish the drink.
, Lactose Tolerance Test - the patient must drink lactose rather than glucose
Bleeding Time Test - This is a timed test and phlebotomist will blot blood every 30
seconds until patient stops bleeding.
Arterial Blood Gas - Use Modified Allen Test to see if patient has adequate circulation
EDTA and NA Citrate - Anticlotting chemicals
Serum Separator gels - used to separate red blood cells and clotting factors from serum
to maintain correct chemistry levels found in blood
Lithium Heparin - used as an anticlotting factor and preservative for some chemistry
analysis
Sodium Fluoride - Used to maintain glucose levels
Blood Culture Media - used to nourish microbes in blood culture tubes and bottles for
identification and remediation of infections
Red Tube - No additive required, commonly used for serum determinations in Chemistry
testing and Blood bank testing
Blue tube - Additive used is sodium citrate, commonly used for coagulation tests,
heparin, PT, APTT, fibrinogen
Lavendar and Pink - Additive used is EDTA commonly used for for hematology testing,
CBC, ESR
Darker Green - Additive used is Heparin, a natural anticoagulant that inhibits thrombin.
Commonly used for routine chemistry testing.
Light Green - Additive used is Lithium Heparin. This additive action is as an anticoagulant.
Not to be used for lithium levels. Used for many chemistry tests.
Gray - Additives used are sodium fluoride, commonly used for glucose toleance tests and
lactic acid measurement
Red/Gray/Camouflage top tube - Additives used are clot activator and serum gel
separator, commonly used for chemistry and immunology testing
Blood Culture tubes or bottles - Microbial growth media. There are two major types:
aerobic growth media and anaerobic growth media
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Random Urine Specimens - collected for a urinalysis.
Midstream Specimen - is also called a clean-voided specimen or a clean-catch specimen
The 24-hour specimen - is collected during a 24-hour period. Urine is typically chilled or
refrigerated to prevent the growth of microbes.
Testing for ph - measures if urine is acidic or alkaline
Testing for glucose or ketones - Typically used for testing for diabetes
Testing for blood - injuries or illnesses can cause blood to appear in the urine-
(hematuria)
Testing for blood - Blood can also appear in stools. Ulcers, some forms of cancer, or
hemorrhoids
Culture & Sensitivity - cultivating a specimen in order to determine whether the
developed organism is sensitive to antibiotics
Throat Culture - Throat cultures are used to determine strep throat, whooping cough and
epiglottitis
Wound Culture - Specimens may be collected from animal bites, ulcers, and skin wounds
Urinalysis - is usually conducted on patient's that are pregnant, have an illness, or are
suspected to have abused an illegal substance.
Urine- collection in temp measured cups - Check for clarity, specific gravity, color and
odor
Cerebrospinal Fluid - mostly obtained through a lumbar puncture and looks for cell
counts, chloride, glucose, and total __________
2- hour Post prandial Glucose - Glucose specimen is collected 2 hours after a patient eats
a meal
Glucose Tolerance Test - must fast at least 12 hours before the test. Patients must drink
glucose prior to testing and have 5 minutes to finish the drink.
, Lactose Tolerance Test - the patient must drink lactose rather than glucose
Bleeding Time Test - This is a timed test and phlebotomist will blot blood every 30
seconds until patient stops bleeding.
Arterial Blood Gas - Use Modified Allen Test to see if patient has adequate circulation
EDTA and NA Citrate - Anticlotting chemicals
Serum Separator gels - used to separate red blood cells and clotting factors from serum
to maintain correct chemistry levels found in blood
Lithium Heparin - used as an anticlotting factor and preservative for some chemistry
analysis
Sodium Fluoride - Used to maintain glucose levels
Blood Culture Media - used to nourish microbes in blood culture tubes and bottles for
identification and remediation of infections
Red Tube - No additive required, commonly used for serum determinations in Chemistry
testing and Blood bank testing
Blue tube - Additive used is sodium citrate, commonly used for coagulation tests,
heparin, PT, APTT, fibrinogen
Lavendar and Pink - Additive used is EDTA commonly used for for hematology testing,
CBC, ESR
Darker Green - Additive used is Heparin, a natural anticoagulant that inhibits thrombin.
Commonly used for routine chemistry testing.
Light Green - Additive used is Lithium Heparin. This additive action is as an anticoagulant.
Not to be used for lithium levels. Used for many chemistry tests.
Gray - Additives used are sodium fluoride, commonly used for glucose toleance tests and
lactic acid measurement
Red/Gray/Camouflage top tube - Additives used are clot activator and serum gel
separator, commonly used for chemistry and immunology testing
Blood Culture tubes or bottles - Microbial growth media. There are two major types:
aerobic growth media and anaerobic growth media
