MODULE 5 EXAM
Actual Questions and Verified Answers
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Portage Learning, Geneva College
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➢ Module 5 Exam
➢ True & False Questions
➢ Multiple Choice Questions and Answers
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➢ Expert-Verified Explanations
,1. The concept of treating all samples, whether known or unknown, as
potentially hazardous (or pathogenic) materials is known as _____.
Correct Answer: Universal Precautions
Expert Rationale:
Universal Precautions are an infection control approach developed to treat
all human blood and certain human body fluids as infectious, regardless of
the perceived status of the source individual. This approach minimizes risk
of exposure to bloodborne pathogens and is critical for laboratory and
clinical safety.
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2. Define the concept of universal precautions.
Answer choices:
Correct Answer: . Treat all samples, whether identified or unidentified, as
potentially hazardous or pathogenic materials.
Expert Rationale:
Universal precautions require that all biological samples are handled with
equal caution because the infective status may be unknown. This
protective principle ensures worker and public safety by assuming the
presence of infectious agents in any specimen.
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,3. List at least 3 observations a researcher would be sure to note
while assessing an unknown microbial sample.
Answer choices:
A. Antibiotic resistance, spore formation, pH tolerance
B. Size and shape, observable motility, Gram status, color change,
chemical reactions
C. Viral morphology, nucleic acid type, host range
D. Oxygen requirement, pathogenicity, toxin production
Correct Answer: B. Size and shape, observable motility, Gram status,
color change, chemical reactions
Expert Rationale:
Initial morphological and biochemical observations provide essential clues
to microbial identity and classification. Assessing size, shape, motility,
Gram reaction, and biochemical activity allows microbiologists to narrow
down potential species before molecular confirmation.
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4. While observing an unknown sample of limited amounts, a
researcher must determine the following observations: (1) the
presence of motility and (2) its Gram status using the same sample -
the liquid sample cannot be divided. Which would you determine first
and why?
Answer choices:
A. Gram stain first, then motility test, because Gram stain differentiates cell
wall type.
, B. Motility first, then Gram stain, because heat fixation in Gram stain kills
motile organisms.
C. Either one can be done first; results do not interfere.
D. Neither test can be performed with limited sample.
Correct Answer: B. Motility first, then Gram stain, because heat fixation in
Gram stain kills motile organisms.
Expert Rationale:
Motility must be assessed on live, unfixed specimens (wet mounts)
because heat fixation (used in Gram staining) kills cells and prevents
observation of motile activity. Starting with motility preserves critical
functional information before processing the fixed Gram stain for cell wall
analysis.
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5. A facultative anaerobe is a microorganism capable of growth under
what conditions?
Answer choices:
A. Only in the absence of oxygen (anaerobic conditions)
B. Only in the presence of oxygen (aerobic conditions)
C. Both aerobic and anaerobic conditions
D. Neither aerobic nor anaerobic conditions
Correct Answer: C. Both aerobic and anaerobic conditions
Expert Rationale: