Medical Assistant Exam – Actual 2025 Questions with Fully Explained Answers
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
What are the 3 veins that are located in the antecubital fossa? - ANS-Median cubital vein
-Cephalic vein
-Basilic vein
Tiger Top & Red Top Tube
-Additives?
-Common Tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANSTiger:
Serum Separator tube (SST)
Additive: Glass particles, silica, & thixotropic gel.
Tests: chemistry, lipids, cardiac enzymes (CKMB & Troponin)
Invert: 8 times
Red:
Additive: None
Tests: chemistry
Invert: 0
Chemistry part of the lab
Gray Top Tubes
-Additives?
-Common Tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANSAdditives: Sodium fluoride & Potassium
Oxalate
Common tests: Fasting blood sugar, Glucose tolerance test (GTT), blood alcohol levels, and
lactic acid measurements
Invert: 8 times
Chemistry part of the lab
Green Top Tubes
-Additives?
-Common tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANSAdditives: Heparin, Sodium, lithium or
ammonium-ion
Tests: Ammonia, Carboxyhemoglobin, and STAT electrolytes
,Medical Assistant Exam – Actual 2025 Questions with Fully Explained Answers
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
Invert: 8 times
Chemistry part of the lab
Lavender Top Tube
-Additives?
-Common tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANS**MUST fill tube 2/3**
Additives: EDTA (Ethylenediaminetetraacetic acid)
Tests: CBC (RBC, WBC, and Platelet), WBC differential, Hemoglobin & Hematocrit
determinations, Erythrocyte sedimentation Rate (ESR)/ SED rate, sickle cell screening, and
lead levels.
Invert: 8 times
Hematology part of the lab
Light Blue Top Tube
-Additives?
-Common Tests?
-How many times do you need to invert?
-What part of the lab does this tube go to? - ANS**Needs to be filled completely**
**9 parts blood to 1 part sodium**
Additives: Sodium Citrate
Common tests: Prothrombin Time (PT), Coumadin Therapy, Activated Partial
Thromboplastin Time (PTT), Monitors heparin therapy and bleeding time.
Invert: 3-4 times
Hematology part of the lab
What is the order of draw? - ANS1- Sterile
2- Light Blue
3- Red / Tiger Top
4- Green
5- Lavender
6- Gray
,Medical Assistant Exam – Actual 2025 Questions with Fully Explained Answers
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
What is a hematoma? - ANSThe most common complication in plebotomy. This indicated
that blood has accumulated in the tissue surrounding the vein.
Causes: The needle going through the vein, and failure to apply enough pressure on the site
after needle withdrawal.
What are some factors to consider prior to performing venipuncture? - ANS-Fasting
-Edema
-Fistula
What are petechiae? - ANSTiny non-raised spots that appear on the skin from rupturing of
the capillaries due to the tourniquet being left on too long or too tight.
(Tiny little bruises)
What is the order of draw for capillary specimens? - ANS1. Lavender Tube
2. Tubes with additives
3. Tubes without additives
What is plebitis? - ANSInflammation of a vein as a result of repeated venipuncture on that
vein.
How many blood draws in a 3-hour glucose tolerance test? (GTT) - ANS5 sticks
What are the precordial leads on an EKG? - ANSV1, V2, V3, V4, V5, V6
Where is precordial lead V1 located? - ANSIn the fourth intercostal space, right sternal
border
(Above the right breast)
Where is precordial lead V2 located? - ANSIn the fourth intercostal space, left sternal border
(Above the left breast)
Where is precordial lead V4 located? - ANSIn the fifth intercostal space, left mid-clavicular
line
(Right below the nipple)
Where is precordial lead V5 located? - ANSIn the fifth intercostal space, anterior axillary line
Where is precordial lead V6 located? - ANSFifth intercostal space, mid-axillary line
Under the age of 8 years old, what side of the heart will you perform an EKG on? - ANSThe
right side of the heart
What are the five types of white blood cells (WBCs) in the blood? - ANSNeutrophils
Lymphocytes
Monocytes
Eosinophils
Basophils
Eosinophils - ANS1%-3% of the WBC population
, Medical Assistant Exam – Actual 2025 Questions with Fully Explained Answers
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
Numbers increase in allergies, skin infections, and parasitic infections.
** Think of EOR from Winnie the Pooh*
Monocytes - ANS3% to 8% of the WBC population
Largest of the WBCs
Numbers increase in intracellular infections and tuberculosis
Lymphocytes - ANS20% to 40% of the WBC population
Numbers increase in viral infection
**They play a role in immunity.
Neutrophils - ANS**Most abundant amount of WBCs**
About 40% to 60% of the WBC population
Their numbers increase in bacterial infections
(phagocytic cells)
Basophils - ANS0 to 17% of WBC population.
They carry histamine, which is released in allergic reactions.
What is the bleeding time test & what does it evaluate? - ANSIt is used to evaluate primary
homeostasis, used to see how quickly you would stop bleeding.
Used for surgical purposes!!
What are the five groups of infectious organisms? - ANSViruses
Bacteria
Protozoa
Fungi
Rickettsiae
What are the eight pulse sites? - ANSTemporal
Carotid
Apical
Brachial
Radial
Femoral
Popliteal
Dorsal
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
What are the 3 veins that are located in the antecubital fossa? - ANS-Median cubital vein
-Cephalic vein
-Basilic vein
Tiger Top & Red Top Tube
-Additives?
-Common Tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANSTiger:
Serum Separator tube (SST)
Additive: Glass particles, silica, & thixotropic gel.
Tests: chemistry, lipids, cardiac enzymes (CKMB & Troponin)
Invert: 8 times
Red:
Additive: None
Tests: chemistry
Invert: 0
Chemistry part of the lab
Gray Top Tubes
-Additives?
-Common Tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANSAdditives: Sodium fluoride & Potassium
Oxalate
Common tests: Fasting blood sugar, Glucose tolerance test (GTT), blood alcohol levels, and
lactic acid measurements
Invert: 8 times
Chemistry part of the lab
Green Top Tubes
-Additives?
-Common tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANSAdditives: Heparin, Sodium, lithium or
ammonium-ion
Tests: Ammonia, Carboxyhemoglobin, and STAT electrolytes
,Medical Assistant Exam – Actual 2025 Questions with Fully Explained Answers
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
Invert: 8 times
Chemistry part of the lab
Lavender Top Tube
-Additives?
-Common tests?
-How many times do you invert?
-What part of the lab does this tube go to? - ANS**MUST fill tube 2/3**
Additives: EDTA (Ethylenediaminetetraacetic acid)
Tests: CBC (RBC, WBC, and Platelet), WBC differential, Hemoglobin & Hematocrit
determinations, Erythrocyte sedimentation Rate (ESR)/ SED rate, sickle cell screening, and
lead levels.
Invert: 8 times
Hematology part of the lab
Light Blue Top Tube
-Additives?
-Common Tests?
-How many times do you need to invert?
-What part of the lab does this tube go to? - ANS**Needs to be filled completely**
**9 parts blood to 1 part sodium**
Additives: Sodium Citrate
Common tests: Prothrombin Time (PT), Coumadin Therapy, Activated Partial
Thromboplastin Time (PTT), Monitors heparin therapy and bleeding time.
Invert: 3-4 times
Hematology part of the lab
What is the order of draw? - ANS1- Sterile
2- Light Blue
3- Red / Tiger Top
4- Green
5- Lavender
6- Gray
,Medical Assistant Exam – Actual 2025 Questions with Fully Explained Answers
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
What is a hematoma? - ANSThe most common complication in plebotomy. This indicated
that blood has accumulated in the tissue surrounding the vein.
Causes: The needle going through the vein, and failure to apply enough pressure on the site
after needle withdrawal.
What are some factors to consider prior to performing venipuncture? - ANS-Fasting
-Edema
-Fistula
What are petechiae? - ANSTiny non-raised spots that appear on the skin from rupturing of
the capillaries due to the tourniquet being left on too long or too tight.
(Tiny little bruises)
What is the order of draw for capillary specimens? - ANS1. Lavender Tube
2. Tubes with additives
3. Tubes without additives
What is plebitis? - ANSInflammation of a vein as a result of repeated venipuncture on that
vein.
How many blood draws in a 3-hour glucose tolerance test? (GTT) - ANS5 sticks
What are the precordial leads on an EKG? - ANSV1, V2, V3, V4, V5, V6
Where is precordial lead V1 located? - ANSIn the fourth intercostal space, right sternal
border
(Above the right breast)
Where is precordial lead V2 located? - ANSIn the fourth intercostal space, left sternal border
(Above the left breast)
Where is precordial lead V4 located? - ANSIn the fifth intercostal space, left mid-clavicular
line
(Right below the nipple)
Where is precordial lead V5 located? - ANSIn the fifth intercostal space, anterior axillary line
Where is precordial lead V6 located? - ANSFifth intercostal space, mid-axillary line
Under the age of 8 years old, what side of the heart will you perform an EKG on? - ANSThe
right side of the heart
What are the five types of white blood cells (WBCs) in the blood? - ANSNeutrophils
Lymphocytes
Monocytes
Eosinophils
Basophils
Eosinophils - ANS1%-3% of the WBC population
, Medical Assistant Exam – Actual 2025 Questions with Fully Explained Answers
Grade A+ Assured.
This document is a complete and detailed study guide for the 2025 Medical
Assistant certification exam, featuring actual exam questions and well-
explained A+ answers. It covers phlebotomy, lab procedures, EKG
interpretation, anatomy and physiology, medical terminology, pharmacology,
vital signs, infection control, patient care, and electronic health record (EHR)
usage. The guide also includes tube colours and additives, order of draw, OSHA
safety standards, and real clinical scenarios aligned with exam expectations.
Latest Updated Exam Study Guide 2025.
Numbers increase in allergies, skin infections, and parasitic infections.
** Think of EOR from Winnie the Pooh*
Monocytes - ANS3% to 8% of the WBC population
Largest of the WBCs
Numbers increase in intracellular infections and tuberculosis
Lymphocytes - ANS20% to 40% of the WBC population
Numbers increase in viral infection
**They play a role in immunity.
Neutrophils - ANS**Most abundant amount of WBCs**
About 40% to 60% of the WBC population
Their numbers increase in bacterial infections
(phagocytic cells)
Basophils - ANS0 to 17% of WBC population.
They carry histamine, which is released in allergic reactions.
What is the bleeding time test & what does it evaluate? - ANSIt is used to evaluate primary
homeostasis, used to see how quickly you would stop bleeding.
Used for surgical purposes!!
What are the five groups of infectious organisms? - ANSViruses
Bacteria
Protozoa
Fungi
Rickettsiae
What are the eight pulse sites? - ANSTemporal
Carotid
Apical
Brachial
Radial
Femoral
Popliteal
Dorsal
