Blood Bank - Serologic and Molecular Testing
1. A patient with multiple myeloma has the following reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency - correct answer A
2. An antibody screen utilizing polyethylene glycol (PEG) was performed. Reaction readings of
this antibody screen are analyzed after immediate spin and again at 37°C incubation with
subsequent centrifugation. How will the centrifugation of the tubes affect the patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen - correct answer C
3. What must be true for the antiglobulin phase of the serologic crossmatch to be omitted (i.e.,
immediate spin crossmatch is done)?
A. The antibody screen must be negative and there is not history of detection of unexpected
antibodies.
B. The patient has not been transfused within the past 24 hours.
C. The blood is needed for surgery.
,Blood Bank - Serologic and Molecular Testing
D. There is a history of detection of unexpected antibodies but the current antibody screen is
negative. - correct answer A
4. Which of the following accurately represents reverse typing?
A. Reverse typing is performed using known reagent antisera to detect ABO antigens on the
patient's red blood cells.
B. Reverse typing is performed using known reagent red blood cells to detect ABO antibodies in
the patient's serum or plasma.
C. Reverse typing is performed using known reagent screening cells to detect clinically
significant antibodies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent antisera to phenotype donor red blood
cells for clinically significant antigens. - correct answer B
5. A former patient had an anti-E four years ago, but her antibody panel is now negative. Since
she now needs blood for surgery, what should the blood bank do?
A. Get autologous blood from relatives.
B. Crossmatch E-negative units.
C. Give group O negative whole blood.
D. Give random compatible units. - correct answer B
6. What is the maximum interval during which a recipient sample may be used for compatibility
testing if the patient has recently been transfused or was pregnant within the past 3 months?
A. 24 hours
B. 3 days
C. One week
D. Two weeks - correct answer B
7. A patient transfused with two units of packed cells spiked a fever of 99.5oF and complained
of chills five days after transfusion. The direct antiglobulin test (DAT) was positive with anti-IgG,
but negative with anti-C3d. Compatibility testing was performed on the pre- and post-
transfusion specimens. The post-transfusion specimen was incompatible with one of the donor
units transfused. An antibody screen was done on both the pre- and post-transfusion
, Blood Bank - Serologic and Molecular Testing
specimens. An antibody was detected in the post-transfusion specimen only and identified by
panel studies as anti-Jka. This transfusion reaction is most:
A. Post-transfusion purpura
B. An anaphylactic response
C. An acute hemolytic transfusion reaction
D. A delayed hemolytic transfusion reaction - correct answer D
8.You perform a gel ABO/Rh test on a 45-year-old male patient and you obtain the following
results.
Anti-A - 4+
Anti-B - 4+
Anti-D - 4+
D control - negative
A1 cells - 2+
B cells - 0
What could be the Type/Rh of this patient?
a. AB POS
b. A POS
c. B POS
d. A2B POS - correct answer D
9. When performing an anti-human globulin (AHG) test, it is important to completely wash the
red cells because:
A. Washing eliminates concentrations of unbound antigens.
B. Washing prevents elution of cell-bound antibody.
1. A patient with multiple myeloma has the following reactions in the ABO typing:
Anti-A= w+
Anti-B = w+
Anti-A,B = w+
Auto control = w+
A1 Cells = 4+
B cells = 4+
What is probably causing these results?
A. Rouleaux
B. Subgroup of A
C. Patient has hypogammaglobulinemia
D. Patient has selective IgA deficiency - correct answer A
2. An antibody screen utilizing polyethylene glycol (PEG) was performed. Reaction readings of
this antibody screen are analyzed after immediate spin and again at 37°C incubation with
subsequent centrifugation. How will the centrifugation of the tubes affect the patient's results?
A. Reactions will be enhanced
B. No effect will be seen
C. False positives may be seen
D. False negatives may be seen - correct answer C
3. What must be true for the antiglobulin phase of the serologic crossmatch to be omitted (i.e.,
immediate spin crossmatch is done)?
A. The antibody screen must be negative and there is not history of detection of unexpected
antibodies.
B. The patient has not been transfused within the past 24 hours.
C. The blood is needed for surgery.
,Blood Bank - Serologic and Molecular Testing
D. There is a history of detection of unexpected antibodies but the current antibody screen is
negative. - correct answer A
4. Which of the following accurately represents reverse typing?
A. Reverse typing is performed using known reagent antisera to detect ABO antigens on the
patient's red blood cells.
B. Reverse typing is performed using known reagent red blood cells to detect ABO antibodies in
the patient's serum or plasma.
C. Reverse typing is performed using known reagent screening cells to detect clinically
significant antibodies in the patient's serum or plasma.
D. Reverse typing is performed using known reagent antisera to phenotype donor red blood
cells for clinically significant antigens. - correct answer B
5. A former patient had an anti-E four years ago, but her antibody panel is now negative. Since
she now needs blood for surgery, what should the blood bank do?
A. Get autologous blood from relatives.
B. Crossmatch E-negative units.
C. Give group O negative whole blood.
D. Give random compatible units. - correct answer B
6. What is the maximum interval during which a recipient sample may be used for compatibility
testing if the patient has recently been transfused or was pregnant within the past 3 months?
A. 24 hours
B. 3 days
C. One week
D. Two weeks - correct answer B
7. A patient transfused with two units of packed cells spiked a fever of 99.5oF and complained
of chills five days after transfusion. The direct antiglobulin test (DAT) was positive with anti-IgG,
but negative with anti-C3d. Compatibility testing was performed on the pre- and post-
transfusion specimens. The post-transfusion specimen was incompatible with one of the donor
units transfused. An antibody screen was done on both the pre- and post-transfusion
, Blood Bank - Serologic and Molecular Testing
specimens. An antibody was detected in the post-transfusion specimen only and identified by
panel studies as anti-Jka. This transfusion reaction is most:
A. Post-transfusion purpura
B. An anaphylactic response
C. An acute hemolytic transfusion reaction
D. A delayed hemolytic transfusion reaction - correct answer D
8.You perform a gel ABO/Rh test on a 45-year-old male patient and you obtain the following
results.
Anti-A - 4+
Anti-B - 4+
Anti-D - 4+
D control - negative
A1 cells - 2+
B cells - 0
What could be the Type/Rh of this patient?
a. AB POS
b. A POS
c. B POS
d. A2B POS - correct answer D
9. When performing an anti-human globulin (AHG) test, it is important to completely wash the
red cells because:
A. Washing eliminates concentrations of unbound antigens.
B. Washing prevents elution of cell-bound antibody.
