BIOD 171 Exam 3 Study with
correct answers
True or False: A nanometer (10-9) is longer than a micrometer (10-6). - ANS-False; A
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wnanometer is 1,000 times smaller than a micrometer.
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Resolution and contrast are two critical factors that influence your ability to see an object.
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Explain each. - ANS-Resolution is the difference in distance between two objects that appear
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as two separate objects.
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Contrast is the difference in light absorption by an object.
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Assuming a constant (non-adjustable) light source power, identify the part of the microscope
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you would adjust to limit the amount of light entering the microscope. - ANS-Iris diaphragm
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What is the total magnification (relative to your eye) of a sample imaged with a 60x
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objective and a 10x eyepiece? Show your math. - ANS-60 x 10 = 600x magnification
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True or False: Staining is often required to image a cell that is adherent and flat (thin). -
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wANS-True
Which of the following could NOT be seen clearly by the unaided eye? Select all that apply. -
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ANS-Bacteria with diameter of 24 μm
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Virus with a diameter of 0.2 μm
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This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects. - ANS-
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Fluorescence
This type of microscope can provide detailed images of live cells without staining. - ANS-
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Phase-Contrast
This type of microscope enhances contrast between specimen and background but does not
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wpermit the visualization of intracellular structures. - ANS-Dark Field
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This type of microscope is capable of capturing images in multiple focal planes, rendering a
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wspecimen in 3-D. - ANS-Confocalw w w w
correct answers
True or False: A nanometer (10-9) is longer than a micrometer (10-6). - ANS-False; A
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wnanometer is 1,000 times smaller than a micrometer.
w w w w w w w
Resolution and contrast are two critical factors that influence your ability to see an object.
w w w w w w w w w w w w w w
Explain each. - ANS-Resolution is the difference in distance between two objects that appear
w w w w w w w w w w w w w w
as two separate objects.
w w w w
Contrast is the difference in light absorption by an object.
w w w w w w w w w
Assuming a constant (non-adjustable) light source power, identify the part of the microscope
w w w w w w w w w w w w
you would adjust to limit the amount of light entering the microscope. - ANS-Iris diaphragm
w w w w w w w w w w w w w w w
What is the total magnification (relative to your eye) of a sample imaged with a 60x
w w w w w w w w w w w w w w w
objective and a 10x eyepiece? Show your math. - ANS-60 x 10 = 600x magnification
w w w w w w w w w w w w w w w
True or False: Staining is often required to image a cell that is adherent and flat (thin). -
w w w w w w w w w w w w w w w w w
wANS-True
Which of the following could NOT be seen clearly by the unaided eye? Select all that apply. -
w w w w w w w w w w w w w w w w w
ANS-Bacteria with diameter of 24 μm
w w w w w w
Virus with a diameter of 0.2 μm
w w w w w w
This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects. - ANS-
w w w w w w w w w w w w w
Fluorescence
This type of microscope can provide detailed images of live cells without staining. - ANS-
w w w w w w w w w w w w w w
Phase-Contrast
This type of microscope enhances contrast between specimen and background but does not
w w w w w w w w w w w w
wpermit the visualization of intracellular structures. - ANS-Dark Field
w w w w w w w w
This type of microscope is capable of capturing images in multiple focal planes, rendering a
w w w w w w w w w w w w w w
wspecimen in 3-D. - ANS-Confocalw w w w
