MCDB 427 Exam 1 Experimental Techniques Questions and Answers

MCDB 427 Exam 1 Experimental
Techniques Questions and Answers

cDNA cloning - Correct Answers -Synthesis of cDNA from an mRNA template

RACE - Correct Answers -Used to obtain a full length sequence cDNA

RT-PCR - Correct Answers -Used to clone and amplify a cDNA from an mRNA

qPCR/Real-time PCR - Correct Answers -Used to quantify the amplification of a DNA
molecule

Gel electrophoresis - Correct Answers -Separates DNA by size

Run-on transcription - Correct Answers -Used to determine transcription rates by
isolating nuclei in vivo

Reporter gene transcription - Correct Answers -Used to measure transcription in vivo

Western blot - Correct Answers -Detects specific proteins in a protein sample

Immunoprecipitation - Correct Answers -Used in conjunction with blotting to measure
accumulation of a gene product

Pulsed-field gel electrophoresis - Correct Answers -Allows one to measure the size of
very large DNA

SDS PAGE - Correct Answers -Separates proteins by size

2-D Gel electrophoresis - Correct Answers -Separates proteins by size and charge

Gel filtration chromatography - Correct Answers -Molecules are separated by size

Southern blot - Correct Answers -Detects specific DNA in DNA samples; requires
membrane

Site-directed mutagenesis - Correct Answers -Used to make specific and intentional
changes in DNA sequences

Northern blot - Correct Answers -Detects specific RNA in RNA samples; requires filter