BIO 235 Exam 1 Questions with Correct
Answers
phase contrast microscopy Correct Answ_uses refraction and interference to create high
contrast/ resolution images with staining
Magnification Correct Answ_ability to enlarge objects
Resolution Correct Answ_The ability to distinguish two objects from each other at a given
magnification
objective lens Correct Answ_The lens on a light microscope that is closest to the stage,
ocular lens is 10x
simple microscope Correct Answ_a microscope that contains only one lens
compound microscope Correct Answ_A light microscope that has more than one lens
,acid fast stain Correct Answ_able to differentiate between two gram positive cells ,
carbolfuchsin used as primary stain
counterstain Correct Answ_application of second stain with a contrasting colour to sample
for microscopy
simple stain Correct Answ_a method of staining microorganisms with a single basic dye, all
organisms appear the same color , even if there is more than one type.
differential stain Correct Answ_a procedure that stains specific morphological structures -
usually a multiple stain
positive stain Correct Answ_dye sticks to the specimen and gives it color
negative stain Correct Answ_colors the background, which makes capsules more visible
Gram stain Correct Answ_multi steps, used to distinguish between bacteria with different
types of cell walls
, Gram staining steps Correct Answ_1. crystal violet
2. iodine
3. alcohol
4. safranin
gram positive cells are Correct Answ_purple
gram negative cells are Correct Answ_pink
scanning electron microscope Correct Answ_an electron microscope that generates a three-
dimensional image.
transmission electron microscope Correct Answ_used to study the internal structure of cells
loop Correct Answ_can be used to streak bacteria on agar on a Petri dish
media Correct Answ_used to grow microorganisms in a lab setting
Answers
phase contrast microscopy Correct Answ_uses refraction and interference to create high
contrast/ resolution images with staining
Magnification Correct Answ_ability to enlarge objects
Resolution Correct Answ_The ability to distinguish two objects from each other at a given
magnification
objective lens Correct Answ_The lens on a light microscope that is closest to the stage,
ocular lens is 10x
simple microscope Correct Answ_a microscope that contains only one lens
compound microscope Correct Answ_A light microscope that has more than one lens
,acid fast stain Correct Answ_able to differentiate between two gram positive cells ,
carbolfuchsin used as primary stain
counterstain Correct Answ_application of second stain with a contrasting colour to sample
for microscopy
simple stain Correct Answ_a method of staining microorganisms with a single basic dye, all
organisms appear the same color , even if there is more than one type.
differential stain Correct Answ_a procedure that stains specific morphological structures -
usually a multiple stain
positive stain Correct Answ_dye sticks to the specimen and gives it color
negative stain Correct Answ_colors the background, which makes capsules more visible
Gram stain Correct Answ_multi steps, used to distinguish between bacteria with different
types of cell walls
, Gram staining steps Correct Answ_1. crystal violet
2. iodine
3. alcohol
4. safranin
gram positive cells are Correct Answ_purple
gram negative cells are Correct Answ_pink
scanning electron microscope Correct Answ_an electron microscope that generates a three-
dimensional image.
transmission electron microscope Correct Answ_used to study the internal structure of cells
loop Correct Answ_can be used to streak bacteria on agar on a Petri dish
media Correct Answ_used to grow microorganisms in a lab setting
