Bio 272 Lab Exam 1 with Accurate
solutions
Where does broken glass that has not been in contact with organisms go? - Correct
Answers -In the cardboard disposal box
Where should broken glass that has been in contact with organisms go? - Correct
Answers -In the red biohazard sharps container.
Why is ethanol dangerous in lab? - Correct Answers -It is extremely flammable
what is the definition of disinfection? - Correct Answers -destroys harmful
microorganisms (pathogens), but not endospores.
What is the definition of sterilization? - Correct Answers -to kill a microorganisms
including endospores.
when we clean a surface what two variables can we control. - Correct Answers -
concentration of a cleaner and time that it is left on a surface
why are nonenveloped viruses harder to kill. - Correct Answers -they are harder to kill
because they contain no lipids and therefore react differently than enveloped viruses
that take part of the host's membrane.
Where does media, culture, and petri plates go when they need to be disposed? -
Correct Answers -In the gray container marked discard
Explosive - Correct Answers -Symbol for explosive
Flammable - Correct Answers -Symbol for flammable
Oxidizing - Correct Answers -Symbol for oxidizing
Compressed gas - Correct Answers -symbol for compressed gas
Harmful/Irritant - Correct Answers -Symbol for harmful/irritant
dangerous for the environment - Correct Answers -Symbol for dangerous for the
environment
, Health Hazard - Correct Answers -Symbol for health hazard
Corrosive - Correct Answers -symbol for corrosive
Toxic - Correct Answers -symbol for toxic
What are the steps to the spills procedure? - Correct Answers -1. Alert anyone in the
vicinity of the spill, so they can avoid it
2. Report the spill toy your instructor
3. Place paper towels over the spill and saturate with bleach
4. Put on gloves
5. Wipe the spill up with more paper towels
6. Place paper towels on biohazard container on your bench
7. Wash hands. disinfect clothing if necessary
What is the purpose of inoculating loops/needles? - Correct Answers -Used to transfer
organisms and small amount of fluid
What is the purpose of slide holders? - Correct Answers -to be used in specific staining
procedures
What is the only thing that can be used to clean microscopes? - Correct Answers -lens
paper and ethanol
What is the purpose of bibulous paper? - Correct Answers -to blot dry slides
What is the purpose of the staining bar? - Correct Answers -to hold slides over the sink
at your bench
When should you use a test tube rack? - Correct Answers -Anytime you are moving test
tubes
What is an Infectious Dose? - Correct Answers -A specific number of microorganisms
that are necessary for infection and disease
What is aseptic technique? - Correct Answers -a procedure so as not to introduce any
unwanted microbes
What is a TBAB petri plate? - Correct Answers -a dish that contains nutrients and water.
What is agar? - Correct Answers -a solidifying agent that allows for the growth of most
organisms that can tolerate aerobic conditions
What is nutrient broth? - Correct Answers -Liquid media without agar.
solutions
Where does broken glass that has not been in contact with organisms go? - Correct
Answers -In the cardboard disposal box
Where should broken glass that has been in contact with organisms go? - Correct
Answers -In the red biohazard sharps container.
Why is ethanol dangerous in lab? - Correct Answers -It is extremely flammable
what is the definition of disinfection? - Correct Answers -destroys harmful
microorganisms (pathogens), but not endospores.
What is the definition of sterilization? - Correct Answers -to kill a microorganisms
including endospores.
when we clean a surface what two variables can we control. - Correct Answers -
concentration of a cleaner and time that it is left on a surface
why are nonenveloped viruses harder to kill. - Correct Answers -they are harder to kill
because they contain no lipids and therefore react differently than enveloped viruses
that take part of the host's membrane.
Where does media, culture, and petri plates go when they need to be disposed? -
Correct Answers -In the gray container marked discard
Explosive - Correct Answers -Symbol for explosive
Flammable - Correct Answers -Symbol for flammable
Oxidizing - Correct Answers -Symbol for oxidizing
Compressed gas - Correct Answers -symbol for compressed gas
Harmful/Irritant - Correct Answers -Symbol for harmful/irritant
dangerous for the environment - Correct Answers -Symbol for dangerous for the
environment
, Health Hazard - Correct Answers -Symbol for health hazard
Corrosive - Correct Answers -symbol for corrosive
Toxic - Correct Answers -symbol for toxic
What are the steps to the spills procedure? - Correct Answers -1. Alert anyone in the
vicinity of the spill, so they can avoid it
2. Report the spill toy your instructor
3. Place paper towels over the spill and saturate with bleach
4. Put on gloves
5. Wipe the spill up with more paper towels
6. Place paper towels on biohazard container on your bench
7. Wash hands. disinfect clothing if necessary
What is the purpose of inoculating loops/needles? - Correct Answers -Used to transfer
organisms and small amount of fluid
What is the purpose of slide holders? - Correct Answers -to be used in specific staining
procedures
What is the only thing that can be used to clean microscopes? - Correct Answers -lens
paper and ethanol
What is the purpose of bibulous paper? - Correct Answers -to blot dry slides
What is the purpose of the staining bar? - Correct Answers -to hold slides over the sink
at your bench
When should you use a test tube rack? - Correct Answers -Anytime you are moving test
tubes
What is an Infectious Dose? - Correct Answers -A specific number of microorganisms
that are necessary for infection and disease
What is aseptic technique? - Correct Answers -a procedure so as not to introduce any
unwanted microbes
What is a TBAB petri plate? - Correct Answers -a dish that contains nutrients and water.
What is agar? - Correct Answers -a solidifying agent that allows for the growth of most
organisms that can tolerate aerobic conditions
What is nutrient broth? - Correct Answers -Liquid media without agar.
