bio 272 exam 2 questions and answers
gef - Correct Answers -GTP exchange factor. activates, by phosphoroalting
gap - Correct Answers -GTPase activating protein. inactivates protein by remove p from
gtp
rcc1 - Correct Answers -ran-gef associates with chromosomes during cytokenesis to
keep gef in nucleus
how does ran gap become localized to the cytoplosim - Correct Answers -1. target
mitotic spindle (outside of nucleus)
2. associate with nuclear vesicles
rangtp functions where? - Correct Answers -nucleoplasm
RanGDP function where - Correct Answers -cytoplasm
ERAD ER Associated Degradation - Correct Answers -proteins not folded correctly sent
to 26s protesome. looks at glucose sugars
what causes unfolding - Correct Answers -heat, salt, lack of sugsrs
SNARES - Correct Answers -V-Snare vesicle
T-snare target
help tge recogniztion of endo membrane targets
transient pore is formed how? - Correct Answers -cargo-receptor complex binds to
peroxisomal membrane. the receptor is then recycled
N-glycosylation - Correct Answers -N-X-S/T. a preassembled 14 sugar oligosaccharide.
ONLY OCCURS IN PROTEINS IMPORTED TO ER
Membrane protein sequence - Correct Answers -KKXX Lysine-Lysine cytosolic retention
signal
Calanexin - Correct Answers -N glycosylated proteins with glucose are retained by this
integral membrane protein
Glycosidases - Correct Answers -remove glucose sugars from the N-glycosylation site
forward step in endomembrane system - Correct Answers -secretion anterograde
, retrival in endomembrane system - Correct Answers -retrograde recycling
COP-1 - Correct Answers -Golgi to ER coat protein. recycling
COP 2 - Correct Answers -ER to Golgi coat protein. secratory
clathrin ap1 - Correct Answers -golgi to lysosome. secratory
clathrin ap2 - Correct Answers -pm to endosome
Sar 1-GTPase - Correct Answers -recruit cop2 proteins. inactive cytoplasm, amphiphilic
Sec23/24 - Correct Answers -Inner subunit of COP2. Recruits Sec13/31. sec 23 has an
inner GAP causing disabled of gap 2
Sec 13/31 - Correct Answers -outer coat of cop2. concentrates cargo.
formation of cop2 - Correct Answers -1. sar 1 binds
2.inner coat with sec 24 and sec 23 forms. sar1 binds with sec 23. cargo wirh sec 24
3outercoat with sec 14 and 31 recruited
how to get past nuclear pore - Correct Answers -intrinsiclly disordered proteins
nuclear localization signal recognizes nuclear import receptor
c terminus bilayer travel happens where - Correct Answers -peroxisomal and nuclear
envelope
nuclear lamins - Correct Answers -provide rigidity, and interact with chromosomes
Ran-GTPase - Correct Answers -controls travel at nucleaus through phosphorolation
what state are proteins in when they cross the peroxisomal bilayer? - Correct Answers -
folded
name of peroxisomal pore+ how it forms - Correct Answers -transient pore+ cargo
receptor complex binds with membranes (trans means inbetween stage usually, where
membranes are making contact)
N-glycosylation - Correct Answers -asparagine(N)-X-S-T sequence. attaches at N. 14-
oligosaccaride. only occurs at proteins being imported into ER. Trimmed in ER and
Golgi.
Resident ER proteins - Correct Answers -proteins that remain in ER once made
gef - Correct Answers -GTP exchange factor. activates, by phosphoroalting
gap - Correct Answers -GTPase activating protein. inactivates protein by remove p from
gtp
rcc1 - Correct Answers -ran-gef associates with chromosomes during cytokenesis to
keep gef in nucleus
how does ran gap become localized to the cytoplosim - Correct Answers -1. target
mitotic spindle (outside of nucleus)
2. associate with nuclear vesicles
rangtp functions where? - Correct Answers -nucleoplasm
RanGDP function where - Correct Answers -cytoplasm
ERAD ER Associated Degradation - Correct Answers -proteins not folded correctly sent
to 26s protesome. looks at glucose sugars
what causes unfolding - Correct Answers -heat, salt, lack of sugsrs
SNARES - Correct Answers -V-Snare vesicle
T-snare target
help tge recogniztion of endo membrane targets
transient pore is formed how? - Correct Answers -cargo-receptor complex binds to
peroxisomal membrane. the receptor is then recycled
N-glycosylation - Correct Answers -N-X-S/T. a preassembled 14 sugar oligosaccharide.
ONLY OCCURS IN PROTEINS IMPORTED TO ER
Membrane protein sequence - Correct Answers -KKXX Lysine-Lysine cytosolic retention
signal
Calanexin - Correct Answers -N glycosylated proteins with glucose are retained by this
integral membrane protein
Glycosidases - Correct Answers -remove glucose sugars from the N-glycosylation site
forward step in endomembrane system - Correct Answers -secretion anterograde
, retrival in endomembrane system - Correct Answers -retrograde recycling
COP-1 - Correct Answers -Golgi to ER coat protein. recycling
COP 2 - Correct Answers -ER to Golgi coat protein. secratory
clathrin ap1 - Correct Answers -golgi to lysosome. secratory
clathrin ap2 - Correct Answers -pm to endosome
Sar 1-GTPase - Correct Answers -recruit cop2 proteins. inactive cytoplasm, amphiphilic
Sec23/24 - Correct Answers -Inner subunit of COP2. Recruits Sec13/31. sec 23 has an
inner GAP causing disabled of gap 2
Sec 13/31 - Correct Answers -outer coat of cop2. concentrates cargo.
formation of cop2 - Correct Answers -1. sar 1 binds
2.inner coat with sec 24 and sec 23 forms. sar1 binds with sec 23. cargo wirh sec 24
3outercoat with sec 14 and 31 recruited
how to get past nuclear pore - Correct Answers -intrinsiclly disordered proteins
nuclear localization signal recognizes nuclear import receptor
c terminus bilayer travel happens where - Correct Answers -peroxisomal and nuclear
envelope
nuclear lamins - Correct Answers -provide rigidity, and interact with chromosomes
Ran-GTPase - Correct Answers -controls travel at nucleaus through phosphorolation
what state are proteins in when they cross the peroxisomal bilayer? - Correct Answers -
folded
name of peroxisomal pore+ how it forms - Correct Answers -transient pore+ cargo
receptor complex binds with membranes (trans means inbetween stage usually, where
membranes are making contact)
N-glycosylation - Correct Answers -asparagine(N)-X-S-T sequence. attaches at N. 14-
oligosaccaride. only occurs at proteins being imported into ER. Trimmed in ER and
Golgi.
Resident ER proteins - Correct Answers -proteins that remain in ER once made
