Mammalian Genetics BCH5413 Exam 2 Questions And Answers

Mammalian Genetics BCH5413 Exam 2
Questions And Answers
"Wheretintthetmousetistthistproteintnormallytexpressed?"t-tCORRECTttANSWER-
Whatttypetoftexperimentaltquestiontcouldtyoutanswertbytcreatingtatreportertgenetastattransg
ene?

(1)tBlacktmousetwithtWTtgene;t(2)tbrowntmousetwithtWTtgene;t(3)tbrowntmousetthattistheter
ozygoustfortthetknockouttgene;toffspringtcantbetcreatedtbytcrossingt2toftthetbrowntheterozy
goustmicet-tCORRECTttANSWER-
Whattaretthet3tpossibletoffspringtthattcantbetproducedtfromtatchimerict(knockout)tmousetan
dtatblackt(WT)tmouse?tHowtwilltoffspringtbetcreated?

(1)tNon-
homologoustendtjoiningt(insertions/deletionstoccurtrighttattthetdsDNAtbreak,tproducingtatno
n-
functionaltprotein);t(2)thomologoustdirectedtrepairt(thetendstoftthetdsDNAtbreaktaligntperfec
tly)t-tCORRECTttANSWER-Whattaretthet2tpossibletrepairtmechanismstoftatdouble-
strandtbreak?

(1)tPCR:tSP6tandtT7tprimerstwilltonlytamplifytthettransgenet(bactvector)tandtbeta-
globintprimerstwilltonlytamplifytthetendogenoustgene;t(2)tSoutherntblot:tprobestspecificttoteit
hertthetendogenoustgenetorttransgenetaretusedt-tCORRECTttANSWER-
Oncetantoocytethastbeentinjectedtwithtattransgenetandtimplantedtintotatmouse,twhattaretthet
2tpossibletwaysttotdeterminetwhichtoffspringthavetthettransgene?

(1)tThetgenetcantbetknockedtouttAFTERtinitialtdevelopment,tpreventingtfataltknockoutstfro
mtpreventingtdevelopment;t(2)tthetgenetcantbetknockedtouttonlytintCERTAINttissuest-
tCORRECTttANSWER-

Whattaretthet2tadvantagestoftcreatingtatCre/Loxtknockouttmousetovertthettraditionaltknocko
uttmouse?

(1)tTracrRNAt(bindsttotthet"repeats"tandtactstastatguidetfortrecognition);t(2)tRNasetIIIt(bindstt
otthet"spacer"taftertthetTracrRNAtfortviraltDNAtdegradation)t-tCORRECTttANSWER-
Whatt2tRNAstaretpresenttintthetCas9tcomplext(immunitytphase)?

, 2tdifferenttloxPtsitestflanktexont2toftthettargettgenet(totbetknockedtout)t-
tCORRECTttANSWER-WheretintthetgenometaretloxPtsitestlocatedtintatCre/Loxtmodel?




25%t-tCORRECTttANSWER-
Ift2tbrowntmicetheterozygoustfortthetknockouttgenetaretcrossed,twhatt%tofttheirtoffspringtwillt
bethomozygoustfortthetknockouttgene?

Bacteriat(atparttofttheirtnaturaltimmunity)t-tCORRECTttANSWER-
IntwhichtorganismtwastthetCRISPRtsystemtdiscovered?

Becausetthetsequencestaretthetsame,texcepttthetmutatedtsequencethastatTseItcuttsitet(befo
retdigestion,tthetbandstaretthetsametsizetbecausetthetsequencestaretthetsametlength;taftert
digestion,tthetmutatedtsequencetwastcuttattthetTseItcuttsitetandthastatsmallertband)t-
tCORRECTttANSWER-Mdxtgenetmutation:twhytistthetsuccessfultCRISPR-

Cas9tmutationtdetectedtastatbandtthetsametsizetastthetWTt(undigestedtPCR)tandtastatsmall
ertbandt(TseItdigestedtPCR)?

Bytusingtprobestspecificttoteithertthetendogenoustgenetortthettransgenet-
tCORRECTttANSWER-

Inttransgenictmice,thowtcouldtyoutdeterminetbetweentthetendogenoustgenetandttransgeneti
ntatSoutherntblot?

CD4+t(helper)tT-cellst-tCORRECTttANSWER-
WhichtspecificttypetoftWBCtisttargetedtbytthetHIVtvirus?

Cellstsensitivettotneomycintdidn'ttgettinjectedtwithtthetgenetoftinterestt(theytdon'tthavetneom
ycintresistancetandtwilltdietoff);tcellstsensitivettotganciclovirtcontaintthetTktgenet(thetplasmidt
wastinjectedtintthetwrongtlocation,tbringingtthetTktgenetwithtit)t-tCORRECTttANSWER-
Whentcreatingtknockouttmice,twhytdotcellstneedttotbetisolatedtthattaretsensitivettotG418t(ne
omycin)tANDtganciclovir?

Disruptedt(typicallytbytthetneomycintresistancetgene)t-tCORRECTttANSWER-
Intknockouttmice,tthetgenetis:

Falset(blacktcoattcolortistrecessivetandtbrowntcoattcolortistdominant)t-
tCORRECTttANSWER-T/F:tthetblacktcoattcolortintmicetistdominant.




Falset(bothtatlefttandtrighttZinctFingertaretneededttotcuttBOTHtstrandstoftDNA)t-
tCORRECTttANSWER-T/F:tonlytonetZinctFingertistneededttotproducetatdsDNAtbreak.




Falset(onlytbrowntmicetwillthavetthetknockouttgenetbecausetittwastinjectedtintottheirtEStcells
)t-tCORRECTttANSWER-T/F:tblacktmicetcantcontaintthetknockouttgene.

Falset(SP6tandtT7tsitestaretonlytfoundtintthetbactvector,tnottthetendogenoustgene)t-
tCORRECTttANSWER-