Mammalian Genetics BCH5413 Exam 1 AS Questions And Answers

Mammalian Genetics BCH5413 Exam 1
AS Questions And Answers
"Sea"toftnucleosometmodelt-tCORRECTttANSWER-
Thistnucleosometmodeltoccurstatthightconcentrations,twhentmanytnucleosomestfromtdiffer
enttDNAtstrandstcantinteract.

(1)tAntantibiotictselectiontmarker;t(2)tthetorigintoftreplication;t(3)tatmultipletcloningtsite;t(4)tpri
merst-tCORRECTttANSWER-
WhattdotyoutneedttotperformtSDMt(besidestthetappropriatetvector)?

(1)tBoththavetA/G/Ctastnitrogenoustbases;t(2)tbothtaretcomposedtoftnucleotides;t(3)tbothtar
etatformtoftgenetictmaterial;t(4)tboththavet5't-
>t3'tdirectionality;t(5)tbothtcantformtsecondary/tertiarytstructurest-tCORRECTttANSWER-
Whattaret3tsimilaritiestbetweentDNAtandtRNA?

(1)tCollecttthetinclusiontbodiestandtrefoldtthetprotein;t(2)tusetatlowtcopytnumbertplasmidtvect
ortliketpBR322;t(3)tusetatfusiontproteintliketthetGSTttagttotincreasetsolubility;t(4)treducetthetg
rowthttemperaturet-tCORRECTttANSWER-
Whattaret4tpurificationtmodificationsttottrytiftthetproteintoftinteresttistINSOLUBLE?

(1)tDecreasetsalttcontentt(onlytthetbesttmatchestwilltstaytboundttogether,tnonspecifictbindin
gtwon'ttoccur);t(2)tincreasethybridization/washttempst(onlytthetbesttmatchestwilltstaytboundtt
ogethertbecausethighttempstbreakthydrogentbondstandtnonspecifictinteractions);t(3)tincrea
setdurationtoftwasht(moretlikelyttotwashtawaytnonspecifictbinding)t-tCORRECTttANSWER-
Whattaret3twaysttotincreasetthetstringencytoftathybridizationtintSoutherntblotting?

(1)tDNAthastthyminetandtRNAthasturacil;t(2)tDNAthastant-Htontitst2'tCtandtRNAthastant-
OHtgroup;t(3)tDNAtistnormallytdouble-strandedtwhiletRNAtistnormallytsingle-strandedt-
tCORRECTttANSWER-Whattaret3tdifferencestbetweentDNAtandtRNA?




(1)tDNAtsynthesis/sequencingtcanthappentuninterruptedtbecausetdNTPstcantbetaddedtcon
tinuouslytupontreleasetoftsubsequenttfluorophores;t(2)ttheretistnotstericthinderancetbecaus
etthetfluorophoretistcleavedtbytDNAtpolymerasetupontthetadditiontoftthetnexttdNTPt-
tCORRECTttANSWER-Whattaretthet2tadvantagestoftPacBiotsequencing?




(1)tE.tcolitdoesn'tthavetpost-
translationaltmodifications,tsotsometdownstreamtapplicationstliketphosphorylationtcan'ttbet

,studied;t(2)tthetcDNAtsizetistlimitedttot15tkb,tsotanytplasmidtlargertthantthattwouldtrequiretatdi
fferenttexpressiontsystemt-tCORRECTttANSWER-
Whattaret2treasonstwhytatresearchtmighttwantttotusetantexpressiontsystemtOTHERtthantE.t
coli?

(1)tGCtcontenttshouldtbet40-60%;t(2)tlengthtshouldtbet18-
30tbp;t(3)tthet3'tendtshouldtendtintatGtortCttotpromotetbindingttotthettargettsequencet-
tCORRECTttANSWER-

Whattaretthet3tcharacteristicstoftatprimertthattneedttotbetconsideredtwhentdeterminingtthetT
m?

(1)tGenetexpressiont(encodingtfortspecifictproteins,tcantchangetdependingtontthetenvironm
ent);t(2)tgenetreplicationt(allowstthetpassingtontoftgenetictmaterialtthroughtmitosis/meiosis)t
-tCORRECTttANSWER-Whattaretthet2tfunctionstoftDNAtintcells?

(1)tOverlappingtprimerstwithtthetmutationtintthetmiddle;t(2)tthetoriginaltplasmidtistfromtdam+t
bacteriat-tCORRECTttANSWER-
Whattaretthet2trequirementstfortQuikchangetmutagenesis?

(1)tTaqMantistverytspecifictbecausetthetprobetonlytattachesttotthetsequencetoftinterest;t(2)tt
hetprobestcantbetlabeledtwithtdifferenttdyes,tallowingtfortdetectiontoftmultipletsequencestwit
hintonlytonetreactiont-tCORRECTttANSWER-
Whattaretthet2tadvantagestoftTaqMantovertSYBRtGreen?

(1)tthetddNTPtistlinkedttotdyetthattwilltchangetcolortwhentread;t(2)tthetddNTPthastatreversible
tterminatingtgrouptontthet3'tC,tallowingtfortcontinuoustreadingtoftatsingletstrandt-

tCORRECTttANSWER-Whattaretthet2tmodificationsttotddNTPstintIlluminatsequencing?




(1)tThetplasmidtintthetbacteriophagetvectortistaddedttotatlawntoftE.tcoli;t(2)tThetcoloniestwilltd
ietwheretsuccessfultinfectiontoccurs;t(3)tThetDNAtisttransferredtontotatmembrane;t(4)tSouth
erntblottingtistusedttotprobetthetDNAtoftinterest;t(6)tAutoradiographytistusedtfortdetectiontoftt
hetprobetsignalt-tCORRECTttANSWER-
Howtaretlibrariestscreenedtusingtplaquethybridization?

(1)tThetTOPOtvectortincludestattopoisomerasetthattligatestthetbackbonetandtinsertttogether
;t(2)trestrictiontdigesttpriorttotligationtistnottnecessaryt-tCORRECTttANSWER-
Intwhatt2twaystdidtthetdevelopmenttoftthetTOPOtvectortmaketcloningteasier?

(1)tTheytcouldtcontaintantoriginaltplasmidtthattwasn'ttfullytdigested;t(2)tthetdigestedtplasmidt
couldtligatetitselftbackttogethert-tCORRECTttANSWER-
Intatcloningtexperiment,twhattaret2twaystthattcoloniestcantgrowtontat"notligase/insert"tcontrol
tplate?




(1)tTotintroducetrestrictiontenzymetcuttsites;t(2)ttotintroducetpurificationttags;t(3)ttotstudytthet
relationshiptbetweentproteintstructuretandtfunctiont-tCORRECTttANSWER-
Whattaret3treasonstfortdoingtSDM?

, (1)ttRNAtdoesn'ttneedttotbettranslatedtintotproteinstbecausetitstusedtintthettranslationtproce
ss;t(2)trRNAtmakestuptribosomestandtdoesn'ttneedttotbettranslatedt-tCORRECTttANSWER-
Whattaret2texamplestoftRNAtthattaretexceptionsttotthetcentraltdogma?

1.65t-tCORRECTttANSWER-DNAtistablettotwraptaroundtthethistonesthowtmanyttimes?

1+toftthetPCRtreagentstaretcontaminatedtwithtthettemplatetplasmidt-tCORRECTttANSWER-
IftyoutrantatPCRtreactiontwithouttthettemplatetplasmidtandthadtbandstappeartontatgel,twhattd
oestthistmean?

10tbpt-tCORRECTttANSWER-B-DNAthasthowtmanytbpstperthelicaltturn?

1953t-tCORRECTttANSWER-WhattyeartwastthetstructuretoftDNAtdiscovered?

3.4tAt-tCORRECTttANSWER-
HowtmanytangstromstistthetdistancetbetweentstackedtbasetpairstintDNA?

32Ptdetectiont-tCORRECTttANSWER-
Thisttypetoftprobetdetectiontutilizestatprobetlabeledtwithtatradioactivetmarkertthattistincorpor
atedtuponthybridizationtwithtrandomtprimerstandtthetKlenowtfragment;tthetradioactivitytcant
betmeasuredtfortdetection.

454tpyrosequencingtallowstfortdetectiontthroughtthetreleasetoftLIGHTtupontthetcorrecttbptm
atch;tionttorrenttsequencingtallowstfortdetectiontthroughtatpHtCHANGEtupontthetcorrecttbpt
matcht-tCORRECTttANSWER-
Howtdoestthetmethodtoftbasetdetectiontdiffertbetweent454tpyrosequencingtandtionttorrentts
equencing?

AtandtGt-tCORRECTttANSWER-Whichtbasestaretthetpurines?

Atcontroltplasmidtandtprimerst(fromtthetkit)tthattaretguaranteedttotworktintPCRt-
tCORRECTttANSWER-WhattistthetpositivetcontroltfortPCR?




Atcrossoverteventtbetweentgenestontdifferenttchromosomestintwhichtexonstcantbetexchang
edt-tCORRECTttANSWER-Whattistexontshuffling?

Athousekeepingtproteint(GAPDH,tactin)tthattistexpressedtintalltthetsamplestbeingttestedt-
tCORRECTttANSWER-WhattwouldtbetantidealtloadingtcontroltfortatWesterntblot?




AtlasertexcitestthetddNTPs,tcausingtthemttotemittatcertaintcolortthattcantbetrecordedtandtpie
cedttogethertfortsequencingt(fortthetgel,tthetdifferenttsizestaretreadtfromtsmallestttotlargesttto
tobtaintthetsequence)t-tCORRECTttANSWER-

Howtaretbasestdetectedtintcapillarytelectrophoresistcomparedttotgeltelectrophoresist(intSan
gertsequencing)?