BCH 3033 Exam 1 Questions And Answers

BCH 3033 Exam 1 Questions And
Answers

A-formrDNAr-rCORRECTrrANSWER-
CondensedrformrofrDNA.rDeeperrmajorrgrooverandrshallowerrminorrgroove.

affinityrchromatographyr-rCORRECTrrANSWER-
separationrbasedronraffinityrtorarmolculerattachedrtorcolumnrbead

Anfinsenrexperimentr-rCORRECTrrANSWER-
Therhypothesisrthatr"proteinraminoracidrsequencerdeterminesrtherfinalrshaperarproteinrassu
mesrinrarwaterrsolution"rwasrprovenrtorbercorrectrwhenrChristianrB.rAnfinsenrshowedrthatrifrt
herenzymerribonucleaserwasropenedroutrintorarlinearrchainrandrthenrallowedrtorreform,ritrrea
ssumedrthercorrectrcatalyticrshape.

Urearorrguanidiniumrchloriderdestroyedrallrnoncovalentrbonds,randrtherβ-
mercaptoethanoldestroyedrtherdisulfiderbonds.

B-formrDNAr-rCORRECTrrANSWER-Watson-CrickrmodelrDNA.rDeep,rwidermajorrgroove.

cDNArduplexr-rCORRECTrrANSWER-createdrfromrmRNArbyr
(1)ruserofrreversertranscriptasertorsynthesizerarcDNArstrand
(2)rdigestionrofrtheroriginalrRNArstrand
(3)radditionrofrseveralrGrbasesrtortherDNArbyrterminalrtransferase
(4)rsynthesisrofrarcomplementaryrDNArstrandrusingrthernewlyrsynthesizedrcDNArstrandrasr
artemplate.

Collagenr-rCORRECTrrANSWER-
Collagenrconsistsrofrthreerintertwinedrhelicalrpolypeptiderchainsrthatrformrarsuperhelicarlca
ble.rTherhelicalrpolypeptiderchainsrofrcollagenrarernotrα-helicesrandrarernotrstabilizedrbyrH-
bonds,rbutrratherrstericrrepulsionrofrtherprolinerrings.However,rtherthreerintertwinedrchainsri
nteractrviarH-bonds.

Everyrthirdrresidueisrarglycine.rProlineandrhydroxyproline(Hyp)ralsoarerabundant.

consensusrsequencer-rCORRECTrrANSWER-
arsequencerofrDNArhavingrsimilarrstructurerandrfunctionrinrdifferentrorganisms

, Cosmidsr-rCORRECTrrANSWER-
ArcosmidrisrarhybridrofrλrphagerandrarplasmidrthatrisrusedrtorclonerlargerpiecesrofrDNA.

cumulativerselectionr-rCORRECTrrANSWER-
Partlyrcorrectrfoldingrintermediatesrarerretainedrbecausertheyrarerslightlyrmorerstablerthanr
unfoldedrregions.

dialysisr-rCORRECTrrANSWER-Semipermeablermembranerusedrtordistinguishrbyrsize

differentialrcentrifugationr-rCORRECTrrANSWER-
Procedurerforrseparatingrcellularrcomponentsraccordingrtortheirrsizerandrdensityrbyrspinnin
grarcellrhomogenaterinrarseriesrofrcentrifugerruns.rAfterreachrrun,rthersupernatantrisrremove
drfromrtherdepositedrmaterialr(pellet)randrspunragainratrprogressivelyrhigherrspeeds.

EdmannrDegradationr-rCORRECTrrANSWER-
TheraminoracidrsequencerofrarproteinrcanrberdeterminedrbyrEdmanrdegradation.rTherprotei
nrisrexposedrtorphenylrisothiocyanate(PTH),rwhichrreactsrwithrtherN-
terminalraminoracidrtorformrarPTHrderivative.rTherPTH-
aminoracidrcanrberreleasedrwithoutrcleavingrtherremainderrofrtherprotein,randrtherdegradati
onrisrsubsequentlyrrepeated.

Entropyrofrsystemr-rCORRECTrrANSWER-∆S(surr)r=r-∆H(sys)/T

epitoper-rCORRECTrrANSWER-
therpartrofranrantigenrmoleculertorwhichranrantibodyrattachesritself

firstrlawrofrthermodynamicsr-rCORRECTrrANSWER-
Energyrcanrbertransferredrandrtransformed,rbutritrcannotrbercreatedrorrdestroyed.

Foldingrfunnelr-rCORRECTrrANSWER-

gelrfiltrationrchromatographyr-rCORRECTrrANSWER-
artyperofrcolumnrchromatographyrthatrseparatesrproteinsrbasedronrtheirrsizerusingrsize-
exclusionrbeads;ralsorcalledrsize-exclusionrchromatography.rLargerrmoleculesrelutesrfirst

Gibbsrfreerenergyr-rCORRECTrrANSWER-∆Gr=r∆Hr-rT∆S

Henderson-Hasselbalchrequationr-rCORRECTrrANSWER-pHr=rpKar+rlogr[A-]/[HA]

homodimerr-rCORRECTrrANSWER-
whenrtworpolypeptidesrencodedrbyrthersamergenerbindrtoreachrotherrtorformrardimer.

HPLCr-rCORRECTrrANSWER-separationrbasedronrpolarity

IndirectrELISAr-rCORRECTrrANSWER-