Histology – OSMT UPDATED ACTUAL
Questions and CORRECT Answers
what is the workflow for histology (7) - CORRECT ANSWER - 1) specimen collection
2) grossing
3) fixation
4) processing
5) embedding
6) microtomy
7) routine staining
how are specimens received? - CORRECT ANSWER - they are removed from the body
and sent to receiving area of the lab
-in specimen container
-if the specimen is sent fresh and no fixed they are in saline or saline gauze
characteristics of a specimen container (5) - CORRECT ANSWER - -leak proof, screw cap
lid
-fixative or saline inside
-wide mouth
-WHMIS label
-patient id label
what is on a specimen container label have on it (7) - CORRECT ANSWER - -pt name
-DOB
-hospital #
-collection date
-specimen type
,-ordering doctor
-whmis or ghs label
what should the req match - CORRECT ANSWER - the specimen container and specimen
type
do all the forms for a specimen type being received in the lab have to match ? - CORRECT
ANSWER - yes all forms, container and req must match
where do tissue reception come from - CORRECT ANSWER - comes from operating
room ,ER, doctor office and clinics
what is each specimen given thats unique when its being recieved into the lab - CORRECT
ANSWER - given an unique assesion #
each specimen is checked to make sure it all confirms
what would be an example of an unique assesion number for a specimen coming from autopsy -
CORRECT ANSWER - A2018-3
what are specimens called in order to remove it , it has to be done by surgery - CORRECT
ANSWER - irretrievable specimen
what is grossing - CORRECT ANSWER - looking at the sample in greater detail
-examining and describing
what are the 7 key components in grossing - CORRECT ANSWER - 1) # of pieces
2) size
, 3) weight
4) colour
5) texture/consistency
6) cut surface
7) what is being submitted for work up
what are block sections - CORRECT ANSWER - sections that are cut from the tissue that
are being examined for interest
what is the thickness of an ideal block section - CORRECT ANSWER - 3-4 mm thick
where are block sections placed into - CORRECT ANSWER - cassettes
what are some specimens (objects) that are not processed - CORRECT ANSWER - bullets,
implants, impaled objects
what is fixation - CORRECT ANSWER - to examine tissue thoroughly to harden and
preserve
what is the aim of fixation - CORRECT ANSWER - to preserve the tissue in a lifelike
manner
what the key of fixation - CORRECT ANSWER - stabilize proteins
what is the goal of fixation - CORRECT ANSWER - to prevent putrefaction which is the
destruction of cells by bacterial pathogens or normal flora
what are the effects of fixation (4) - CORRECT ANSWER - 1) hardens tissue
2) makes tissue easier to work with
Questions and CORRECT Answers
what is the workflow for histology (7) - CORRECT ANSWER - 1) specimen collection
2) grossing
3) fixation
4) processing
5) embedding
6) microtomy
7) routine staining
how are specimens received? - CORRECT ANSWER - they are removed from the body
and sent to receiving area of the lab
-in specimen container
-if the specimen is sent fresh and no fixed they are in saline or saline gauze
characteristics of a specimen container (5) - CORRECT ANSWER - -leak proof, screw cap
lid
-fixative or saline inside
-wide mouth
-WHMIS label
-patient id label
what is on a specimen container label have on it (7) - CORRECT ANSWER - -pt name
-DOB
-hospital #
-collection date
-specimen type
,-ordering doctor
-whmis or ghs label
what should the req match - CORRECT ANSWER - the specimen container and specimen
type
do all the forms for a specimen type being received in the lab have to match ? - CORRECT
ANSWER - yes all forms, container and req must match
where do tissue reception come from - CORRECT ANSWER - comes from operating
room ,ER, doctor office and clinics
what is each specimen given thats unique when its being recieved into the lab - CORRECT
ANSWER - given an unique assesion #
each specimen is checked to make sure it all confirms
what would be an example of an unique assesion number for a specimen coming from autopsy -
CORRECT ANSWER - A2018-3
what are specimens called in order to remove it , it has to be done by surgery - CORRECT
ANSWER - irretrievable specimen
what is grossing - CORRECT ANSWER - looking at the sample in greater detail
-examining and describing
what are the 7 key components in grossing - CORRECT ANSWER - 1) # of pieces
2) size
, 3) weight
4) colour
5) texture/consistency
6) cut surface
7) what is being submitted for work up
what are block sections - CORRECT ANSWER - sections that are cut from the tissue that
are being examined for interest
what is the thickness of an ideal block section - CORRECT ANSWER - 3-4 mm thick
where are block sections placed into - CORRECT ANSWER - cassettes
what are some specimens (objects) that are not processed - CORRECT ANSWER - bullets,
implants, impaled objects
what is fixation - CORRECT ANSWER - to examine tissue thoroughly to harden and
preserve
what is the aim of fixation - CORRECT ANSWER - to preserve the tissue in a lifelike
manner
what the key of fixation - CORRECT ANSWER - stabilize proteins
what is the goal of fixation - CORRECT ANSWER - to prevent putrefaction which is the
destruction of cells by bacterial pathogens or normal flora
what are the effects of fixation (4) - CORRECT ANSWER - 1) hardens tissue
2) makes tissue easier to work with
