TEST BANK
Lewin's GENES XII
Jocelyn E. Krebs, Elliott S. Goldstein, and Stephen T. Kilpatrick
1st Edition
,Table of Contents
Chapter 01 Genes Are DNA and Encode RNAs and Polypeptides 1
Chapter 02 Methods in Molecular Biology and Genetic Engineering 10
Chapter 03 The Interrupted Gene 16
Chapter 04 The Content of the Genome 22
Chapter 05 Genome Sequences and Evolution 28
Chapter 06 Clusters and Repeats 37
Chapter 07 Chromosomes 43
Chapter 08 Chromatin 49
Chapter 09 Replication is Connected to the Cell Cycle 55
Chapter 10 The Replicon-Initiation of Replication 61
Chapter 11 DNA Replication 67
Chapter 12 Extrachromosomal Replicons 73
Chapter 13 Homologous and Site-Specific Recombination 79
Chapter 14 Repair Systems 85
Chapter 15 Transposable Elements and Retroviruses 91
Chapter 16 Somatic DNA Recombination and Hypermutation in the Immune System 97
Chapter 17 Prokaryotic Transcription 103
Chapter 18 Eukaryotic Transcription 109
Chapter 19 RNA Splicing and Processing 115
Chapter 20 mRNA Stability and Localization 121
Chapter 21 Catalytic RNA 127
Chapter 22 Translation 132
Chapter 23 Using the Genetic Code 138
Chapter 24 The Operon 144
Chapter 25 Phage Strategies 150
Chapter 26 Eukaryotic Transcription Regulation 155
Chapter 27 Epigenetics I 161
Chapter 28 Epigenetics II 165
Chapter 29 Noncoding RNA 169
Chapter 30 Regulatory RNA 173
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Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
Chapter 01 Genes Are DNA and Encode RNAs and Polypeptides
Multiple Choice
1. The complete set of hereditary information carried by an organism is its:
A) chromosome.
B) genome.
C) double helix.
D) gene.
E) nucleotide.
Ans: B
2. Hershey and Chase tracked DNA during phage infection using a radioisotope of:
A) oxygen.
B) sulfur.
C) nitrogen.
D) phosphorus.
E) carbon.
Ans: D
3. The process of ___________ in eukaryotic cells is analogous to bacterial transformation.
A) transfection
B) transcription
C) transition
D) translation
E) translocation
Ans: A
4. In a polynucleotide, a phosphate group is linked to the ________ carbons of two pentoses.
A) 1′ and 2′
B) 1′ and 3′
C) 3′ and 5′
D) 1′ and 5′
E) 2′ and 3′
Ans: C
5. In DNA, the number of times one strand crosses over the other in space is its:
A) twisting number.
B) positive supercoiling number.
C) writhing number.
D) negative supercoiling number.
E) linking number.
Ans: E
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Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
6. In the B-form of DNA:
A) the two polynucleotide chains are described as parallel.
B) base pairs lie perpendicular to the sugar–phosphate backbone.
C) the width of the helix varies between A-T and G-C base pairs.
D) purine bases pair with other purines.
E) there are approximately 12 base pairs per turn of the helix.
Ans: B
7. DNA replication is semiconservative, meaning that:
A) one of the two daughter duplexes consists of both original parental strands.
B) for each daughter duplex, only one of the parental strands is used as a template for both
daughter strands.
C) each daughter duplex may differ in sequence from the parental duplex.
D) each daughter duplex consists of one parental strand and one newly synthesized daughter
strand.
E) each daughter duplex has sections of original parental duplex and sections of newly
synthesized daughter duplex.
Ans: D
8. Which of the following is a transition mutation?
A) A-T → G-C
B) A-T → C-G
C) A-T → T-A
D) G-C → C-G
E) G-C → T-A
Ans: A
9. As compared to the original mutation, a suppression mutation occurs:
A) in the homologous allele of the same gene.
B) at the same site.
C) in another nonhomologous gene.
D) in a different codon of the same gene.
E) in another site of the same codon.
Ans: C
10. A viroid is:
A) RNA encapsulated in protein.
B) double-stranded DNA encapsulated in protein.
C) a naked DNA molecule.
D) single-stranded DNA encapsulated in protein.
E) a naked RNA molecule.
Ans: E
11. A chromosome is best defined as:
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,______________________________________________________________________________________________
Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
A) the complete sequence of a gene, including sequences removed from the RNA product.
B) the functional coding sequences of a single DNA molecule.
C) the complete set of hereditary information of an organism.
D) a linear array of genes.
E) the sequence of a gene that has coding information for the polypeptide product.
Ans: D
12. Mutation is random with respect to the structure and function of a gene. As a result,
A) the effects of a particular mutation cannot be predicted.
B) mutations are likely to impair the function of a gene.
C) mutations are equally likely at every nucleotide of the gene.
D) mutations invariably destroy the function of the gene.
E) mutations are likely to improve the function of a gene.
Ans: B
13. In the complementation test, crossing two homozygous recessive individuals with similar
phenotypes to yield all wild-type offspring means that:
A) the two parents carry different mutations of the same nucleotide.
B) the two parents’ mutations are in the same gene.
C) the two parents’ mutations affect different traits.
D) the two parents carry identical mutations.
E) the two parents’ mutations are in different genes.
Ans: E
14. Mutations that have no apparent effect are called:
A) silent mutations.
B) loss-of-function mutations.
C) null mutations.
D) leaky mutations.
E) gain-of-function mutations.
Ans: A
15. Genes that are on opposite ends of a long eukaryotic chromosome:
A) never recombine.
B) show less than 50% recombination.
C) show exactly 50% recombination.
D) show between 50% and 100% recombination.
E) show 100% recombination.
Ans: C
16. Full or partial reversion of a deletion mutation may be accomplished by:
A) an insertion mutation in another gene.
B) an insertion mutation close to the original deletion.
C) a substitution mutation close to the original deletion.
D) a second deletion mutation close to the original deletion.
E) a second deletion mutation in another gene.
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Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
Ans: B
17. A closed reading frame:
A) contains frequent stop codons.
B) contains many substitution mutations.
C) is formed by the deletion of a sequence from an open reading frame.
D) begins and ends with the same sequence.
E) produces a polypeptide that serves no apparent function.
Ans: A
18. The colinearity of prokaryotic genes means that:
A) within a prokaryotic gene, both DNA strands encode the same polypeptide.
B) a prokaryotic polypeptide has exactly 3x as many amino acids as the number of nucleotides in
the gene encoding it.
C) prokaryotic proteins are identical in length to their genes.
D) prokaryotic genes contain a continuous unbroken sequence encoding a polypeptide.
E) a physical map of a gene will not match the amino acid map of its polypeptide product.
Ans: D
19. The production of RNA from a DNA template is called:
A) splicing.
B) translation.
C) RNA processing.
D) transport.
E) transcription.
Ans: E
20. A regulatory control site of a gene:
A) must be very close to the gene it affects.
B) must lie upstream of the gene it affects.
C) is cis-acting.
D) is trans-acting.
E) must be very distant from the gene it affects.
Ans: C
Short Answer
1. The basic building block of nucleic acids is the nucleotide. It is composed of three components.
What are they?
Ans: They are a nitrogenous base, a sugar, and a phosphate.
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Lewin's GENES XII
Jocelyn E. Krebs, Elliott S. Goldstein, and Stephen T. Kilpatrick
1st Edition
,Table of Contents
Chapter 01 Genes Are DNA and Encode RNAs and Polypeptides 1
Chapter 02 Methods in Molecular Biology and Genetic Engineering 10
Chapter 03 The Interrupted Gene 16
Chapter 04 The Content of the Genome 22
Chapter 05 Genome Sequences and Evolution 28
Chapter 06 Clusters and Repeats 37
Chapter 07 Chromosomes 43
Chapter 08 Chromatin 49
Chapter 09 Replication is Connected to the Cell Cycle 55
Chapter 10 The Replicon-Initiation of Replication 61
Chapter 11 DNA Replication 67
Chapter 12 Extrachromosomal Replicons 73
Chapter 13 Homologous and Site-Specific Recombination 79
Chapter 14 Repair Systems 85
Chapter 15 Transposable Elements and Retroviruses 91
Chapter 16 Somatic DNA Recombination and Hypermutation in the Immune System 97
Chapter 17 Prokaryotic Transcription 103
Chapter 18 Eukaryotic Transcription 109
Chapter 19 RNA Splicing and Processing 115
Chapter 20 mRNA Stability and Localization 121
Chapter 21 Catalytic RNA 127
Chapter 22 Translation 132
Chapter 23 Using the Genetic Code 138
Chapter 24 The Operon 144
Chapter 25 Phage Strategies 150
Chapter 26 Eukaryotic Transcription Regulation 155
Chapter 27 Epigenetics I 161
Chapter 28 Epigenetics II 165
Chapter 29 Noncoding RNA 169
Chapter 30 Regulatory RNA 173
,______________________________________________________________________________________________
Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
Chapter 01 Genes Are DNA and Encode RNAs and Polypeptides
Multiple Choice
1. The complete set of hereditary information carried by an organism is its:
A) chromosome.
B) genome.
C) double helix.
D) gene.
E) nucleotide.
Ans: B
2. Hershey and Chase tracked DNA during phage infection using a radioisotope of:
A) oxygen.
B) sulfur.
C) nitrogen.
D) phosphorus.
E) carbon.
Ans: D
3. The process of ___________ in eukaryotic cells is analogous to bacterial transformation.
A) transfection
B) transcription
C) transition
D) translation
E) translocation
Ans: A
4. In a polynucleotide, a phosphate group is linked to the ________ carbons of two pentoses.
A) 1′ and 2′
B) 1′ and 3′
C) 3′ and 5′
D) 1′ and 5′
E) 2′ and 3′
Ans: C
5. In DNA, the number of times one strand crosses over the other in space is its:
A) twisting number.
B) positive supercoiling number.
C) writhing number.
D) negative supercoiling number.
E) linking number.
Ans: E
______________________________________________________________________________________________
1|Page
,______________________________________________________________________________________________
Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
6. In the B-form of DNA:
A) the two polynucleotide chains are described as parallel.
B) base pairs lie perpendicular to the sugar–phosphate backbone.
C) the width of the helix varies between A-T and G-C base pairs.
D) purine bases pair with other purines.
E) there are approximately 12 base pairs per turn of the helix.
Ans: B
7. DNA replication is semiconservative, meaning that:
A) one of the two daughter duplexes consists of both original parental strands.
B) for each daughter duplex, only one of the parental strands is used as a template for both
daughter strands.
C) each daughter duplex may differ in sequence from the parental duplex.
D) each daughter duplex consists of one parental strand and one newly synthesized daughter
strand.
E) each daughter duplex has sections of original parental duplex and sections of newly
synthesized daughter duplex.
Ans: D
8. Which of the following is a transition mutation?
A) A-T → G-C
B) A-T → C-G
C) A-T → T-A
D) G-C → C-G
E) G-C → T-A
Ans: A
9. As compared to the original mutation, a suppression mutation occurs:
A) in the homologous allele of the same gene.
B) at the same site.
C) in another nonhomologous gene.
D) in a different codon of the same gene.
E) in another site of the same codon.
Ans: C
10. A viroid is:
A) RNA encapsulated in protein.
B) double-stranded DNA encapsulated in protein.
C) a naked DNA molecule.
D) single-stranded DNA encapsulated in protein.
E) a naked RNA molecule.
Ans: E
11. A chromosome is best defined as:
______________________________________________________________________________________________
2|Page
,______________________________________________________________________________________________
Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
A) the complete sequence of a gene, including sequences removed from the RNA product.
B) the functional coding sequences of a single DNA molecule.
C) the complete set of hereditary information of an organism.
D) a linear array of genes.
E) the sequence of a gene that has coding information for the polypeptide product.
Ans: D
12. Mutation is random with respect to the structure and function of a gene. As a result,
A) the effects of a particular mutation cannot be predicted.
B) mutations are likely to impair the function of a gene.
C) mutations are equally likely at every nucleotide of the gene.
D) mutations invariably destroy the function of the gene.
E) mutations are likely to improve the function of a gene.
Ans: B
13. In the complementation test, crossing two homozygous recessive individuals with similar
phenotypes to yield all wild-type offspring means that:
A) the two parents carry different mutations of the same nucleotide.
B) the two parents’ mutations are in the same gene.
C) the two parents’ mutations affect different traits.
D) the two parents carry identical mutations.
E) the two parents’ mutations are in different genes.
Ans: E
14. Mutations that have no apparent effect are called:
A) silent mutations.
B) loss-of-function mutations.
C) null mutations.
D) leaky mutations.
E) gain-of-function mutations.
Ans: A
15. Genes that are on opposite ends of a long eukaryotic chromosome:
A) never recombine.
B) show less than 50% recombination.
C) show exactly 50% recombination.
D) show between 50% and 100% recombination.
E) show 100% recombination.
Ans: C
16. Full or partial reversion of a deletion mutation may be accomplished by:
A) an insertion mutation in another gene.
B) an insertion mutation close to the original deletion.
C) a substitution mutation close to the original deletion.
D) a second deletion mutation close to the original deletion.
E) a second deletion mutation in another gene.
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Test Bank - Lewin's GENES XII, 1st Edition (Krebs, 2018)
Ans: B
17. A closed reading frame:
A) contains frequent stop codons.
B) contains many substitution mutations.
C) is formed by the deletion of a sequence from an open reading frame.
D) begins and ends with the same sequence.
E) produces a polypeptide that serves no apparent function.
Ans: A
18. The colinearity of prokaryotic genes means that:
A) within a prokaryotic gene, both DNA strands encode the same polypeptide.
B) a prokaryotic polypeptide has exactly 3x as many amino acids as the number of nucleotides in
the gene encoding it.
C) prokaryotic proteins are identical in length to their genes.
D) prokaryotic genes contain a continuous unbroken sequence encoding a polypeptide.
E) a physical map of a gene will not match the amino acid map of its polypeptide product.
Ans: D
19. The production of RNA from a DNA template is called:
A) splicing.
B) translation.
C) RNA processing.
D) transport.
E) transcription.
Ans: E
20. A regulatory control site of a gene:
A) must be very close to the gene it affects.
B) must lie upstream of the gene it affects.
C) is cis-acting.
D) is trans-acting.
E) must be very distant from the gene it affects.
Ans: C
Short Answer
1. The basic building block of nucleic acids is the nucleotide. It is composed of three components.
What are they?
Ans: They are a nitrogenous base, a sugar, and a phosphate.
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