Chapter 9 Biotechnology and DNA Technology

Chapter 9 Biotechnology and DNA Technology

9.1 Multiple-Choice Questions

1) The following are steps used to make DNA fingerprints. What is the third step? A) Collect
DNA.
B) Digest with a restriction enzyme. C) Perform electrophoresis.
D) Lyse cells.
E) Add stain. Answer: B Section: 9.4
Bloom's Taxonomy: Remembering
ASMcue Outcome: 8.3
Learning Outcome: 9.18
Figure 9.1




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2) How many pieces will EcoRI produce from the plasmid shown in Figure 9.1? A) 1
B) 2
C) 3
D) 4
E) 5
Answer: B Section: 9.2
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 4.5
Learning Outcome: 9.4
Global Outcome: 2

3) In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest
piece containing the entire ampicillin-resistance (amp) gene?
A) 0.17 kbp B) 0.25 kbp C) 1.08 kbp D) 1.50 kbp E) 3.00 kbp Answer: D Section: 9.2
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 4.5
Learning Outcome: 9.4

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Global Outcome: 2

Figure 9.2




4) In Figure 9.2, the enzyme in step 1 is
A) DNA polymerase. B) DNA ligase.
C) RNA polymerase.
D) reverse transcriptase. E) spliceosome.
Answer: C Section: 9.3
Bloom's Taxonomy: Understanding
ASMcue Outcome: 4.2
Learning Outcome: 9.10


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