MLT - Microbiology Exam Solution Manual Fully Solved Latest Update 2025
What term describes the morphology of organisms that vary in size and shape? - Answers pleomorphic
Capsules are the basis of which test? - Answers serotyping
A mucoid colony on blood agar medium indicates that which characteristic may be present? - Answers
organism has a capsule
What are dark staining granules called? - Answers metachromatic
Which organisms are non motile? - Answers Shigella and Klebsiella
In the bacterial growth cycle, growth ceases because nutrients are exhausted or toxic metabolic
products have accumulated in what phase? - Answers stationary phase
What genera of bacteria form spores? - Answers Bacillus, Clostridium
What term is used to describe bacteria that grow in the absence of atmospheric oxygen and obtain
oxygen from oxygen-containing compounds? - Answers anaerobes
What is the temperature at which bacteria grow best known as? - Answers optium
On blood sugar plates a small zone of alpha hemolysis surrounded by a zone of beta hemolysis after
refrigeration is known as what type of hemolysis? - Answers alpha-prime
What is the most effective method of sterilization? - Answers autoclaving
What is used for quality control for the dry heat oven? - Answers Bacillus subtilis
Media that cannot be heated can be sterilized by what alternate method? - Answers filtration
What disinfectant is the most effective germicide against bacteria, spores tubercle bacilli, fungi, and
viruses? - Answers chlorine bleach
What term describes a process or treatment that renders a medical device, instrument, or
environmental surface safe to handle? - Answers decontamination
What color do Gram-positive bacteria stain? - Answers purple/ dark blue
Acid-fast bacteria appear what color microscopically? - Answers red
Acid-fast organisms in tissue are best stained by what method? - Answers Kinyoun
What should be used to remove antimicrobials before culturing? - Answers antimicrobial removal device
Name some characteristics concerning sodium polyanethol sulfonate (SPS)- - Answers prevents blood
from clotting, prevents phagocytosis, neutralizes the bactericidal effect on human serum
, What is the most abundant normal flora in throat culture? - Answers alpha-hemolytic streptococci
What is the most common pathogen in throat cultures? - Answers Streptococcus pyogenes
Why is it recommended to stab the blood agar plate when setting up throat cultures? - Answers some
streptococci may not produce beta hemolysis under aerobic conditions
Nasopharyngeal swabs are recommended for the detection of which carriers - Answers H. influenzae, N.
meningitidis, B. pertussis
Why is a first morning urine specimen preferred for urine cultures? - Answers specimen is more
concentrated
What indicates vaginal or urethral contamination of urine? - Answers greater than 10 squamous
epithelial cells per hpf
How are smears of cerebrospinal fluid prepared? - Answers centrifuged CSF sediment
Smears of cerebrospinal fluid are usually stained with which stain? - Answers Gram stain
Which test for bacterial antigen on cultures isolated from CSF is more sensitive and faster? - Answers
latex agglutination tests
What is not the cause of venereal disease? - Answers Haemophilus aegyptius
A positive coagulase test is observed for what? - Answers clotting of plasma
What is the preferred method of differentiating S. aureus from S. epidermidis? - Answers coagulase test
A colony of nonhemolytic, catalase-positive, coagulase-negative, GPC is most likely to be what
organism? - Answers S. epidermidis
When performing a bacitracin differentiation test for Group A beta-hemolytic streptococci, disks should
be used that have how many units? - Answers 0.02-0.04 U
Hippurate hydrolysis is used to differentiate what organism? - Answers group A streptococci from group
B streptococci
If pneumococci are resistant to penicillin, then they should be tested for what? - Answers beta-
lactamase production
What is the most probable identity of bile-esculin positive, beta-hemolytic streptococci that grows in
6.5% NaCl? - Answers enterococci
The bile-esculin test is used to differentiate which two organisms? - Answers Group D streptococci from
enterococci
What term describes the morphology of organisms that vary in size and shape? - Answers pleomorphic
Capsules are the basis of which test? - Answers serotyping
A mucoid colony on blood agar medium indicates that which characteristic may be present? - Answers
organism has a capsule
What are dark staining granules called? - Answers metachromatic
Which organisms are non motile? - Answers Shigella and Klebsiella
In the bacterial growth cycle, growth ceases because nutrients are exhausted or toxic metabolic
products have accumulated in what phase? - Answers stationary phase
What genera of bacteria form spores? - Answers Bacillus, Clostridium
What term is used to describe bacteria that grow in the absence of atmospheric oxygen and obtain
oxygen from oxygen-containing compounds? - Answers anaerobes
What is the temperature at which bacteria grow best known as? - Answers optium
On blood sugar plates a small zone of alpha hemolysis surrounded by a zone of beta hemolysis after
refrigeration is known as what type of hemolysis? - Answers alpha-prime
What is the most effective method of sterilization? - Answers autoclaving
What is used for quality control for the dry heat oven? - Answers Bacillus subtilis
Media that cannot be heated can be sterilized by what alternate method? - Answers filtration
What disinfectant is the most effective germicide against bacteria, spores tubercle bacilli, fungi, and
viruses? - Answers chlorine bleach
What term describes a process or treatment that renders a medical device, instrument, or
environmental surface safe to handle? - Answers decontamination
What color do Gram-positive bacteria stain? - Answers purple/ dark blue
Acid-fast bacteria appear what color microscopically? - Answers red
Acid-fast organisms in tissue are best stained by what method? - Answers Kinyoun
What should be used to remove antimicrobials before culturing? - Answers antimicrobial removal device
Name some characteristics concerning sodium polyanethol sulfonate (SPS)- - Answers prevents blood
from clotting, prevents phagocytosis, neutralizes the bactericidal effect on human serum
, What is the most abundant normal flora in throat culture? - Answers alpha-hemolytic streptococci
What is the most common pathogen in throat cultures? - Answers Streptococcus pyogenes
Why is it recommended to stab the blood agar plate when setting up throat cultures? - Answers some
streptococci may not produce beta hemolysis under aerobic conditions
Nasopharyngeal swabs are recommended for the detection of which carriers - Answers H. influenzae, N.
meningitidis, B. pertussis
Why is a first morning urine specimen preferred for urine cultures? - Answers specimen is more
concentrated
What indicates vaginal or urethral contamination of urine? - Answers greater than 10 squamous
epithelial cells per hpf
How are smears of cerebrospinal fluid prepared? - Answers centrifuged CSF sediment
Smears of cerebrospinal fluid are usually stained with which stain? - Answers Gram stain
Which test for bacterial antigen on cultures isolated from CSF is more sensitive and faster? - Answers
latex agglutination tests
What is not the cause of venereal disease? - Answers Haemophilus aegyptius
A positive coagulase test is observed for what? - Answers clotting of plasma
What is the preferred method of differentiating S. aureus from S. epidermidis? - Answers coagulase test
A colony of nonhemolytic, catalase-positive, coagulase-negative, GPC is most likely to be what
organism? - Answers S. epidermidis
When performing a bacitracin differentiation test for Group A beta-hemolytic streptococci, disks should
be used that have how many units? - Answers 0.02-0.04 U
Hippurate hydrolysis is used to differentiate what organism? - Answers group A streptococci from group
B streptococci
If pneumococci are resistant to penicillin, then they should be tested for what? - Answers beta-
lactamase production
What is the most probable identity of bile-esculin positive, beta-hemolytic streptococci that grows in
6.5% NaCl? - Answers enterococci
The bile-esculin test is used to differentiate which two organisms? - Answers Group D streptococci from
enterococci
