Biology 1402 Lab Midterm
1. Bacterial Colony: A single bacterium that multiplies into millions of cells
2. Bacterial Morphology: Circular, Filamentous, Irregular
3. Gram Stain: Used to identify a bacterium
4. Positive Gram Stain: a simple cell wall, thick peptidoglycan layer, stains
blue/purple
5. Negative Gram Stain: more complex cell wall, thinner peptidoglycan layer,
thick lipopolysaccharide layer, stains red/pink
6. Bacilla: Rods
7. Cocci: spherical
8. Spirilla: spirals
9. Aseptic techniques for colony isolation:
1. clean work space
2. disinfect loop with wipe
3. dip in ethanol
4. flame
5. open culture (hold top with pinkie) and dip in culture
6. zip zag across half of plate (open at 45 degree angle)
7. repeat 3 and 4
8. zig zag 1/4
9. Repeat 7 and 8
10. Bacterial lawn:
1. clean work space
2. micropippet culture
3. dip rod into ethanol
4. flame
5. spread evenly
11. Antibiotics: a bacterium or fungus used to control bacterial growth
12. Aniceptics: control bacteria on living tissue
13. Disinfectant: control bacteria on object
14. Moss: nonvascular bryophyte
1/3
1. Bacterial Colony: A single bacterium that multiplies into millions of cells
2. Bacterial Morphology: Circular, Filamentous, Irregular
3. Gram Stain: Used to identify a bacterium
4. Positive Gram Stain: a simple cell wall, thick peptidoglycan layer, stains
blue/purple
5. Negative Gram Stain: more complex cell wall, thinner peptidoglycan layer,
thick lipopolysaccharide layer, stains red/pink
6. Bacilla: Rods
7. Cocci: spherical
8. Spirilla: spirals
9. Aseptic techniques for colony isolation:
1. clean work space
2. disinfect loop with wipe
3. dip in ethanol
4. flame
5. open culture (hold top with pinkie) and dip in culture
6. zip zag across half of plate (open at 45 degree angle)
7. repeat 3 and 4
8. zig zag 1/4
9. Repeat 7 and 8
10. Bacterial lawn:
1. clean work space
2. micropippet culture
3. dip rod into ethanol
4. flame
5. spread evenly
11. Antibiotics: a bacterium or fungus used to control bacterial growth
12. Aniceptics: control bacteria on living tissue
13. Disinfectant: control bacteria on object
14. Moss: nonvascular bryophyte
1/3
