ASCP CG Certification Practice 2025
UPDATE LATEST EXAM VERSION MOST
TESTED QUESTIONS WITH VERIFIED
ANSWERS GRADED A+ ASSURED
SUCCESS
What is the best agent to use against mycoplasma? – answer BM cyclin - a combination
of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: -
answer comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - answer Proteolytic enzymes - break down
tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with: - answer Colcemid - mitotic spindle poison that halts cells at
metaphase
Samples that arrive at the cytogenetics lab should first be: - answer Logged in to the
system
Phytohemagglutinin (PHA) stimulates which type of cells into division? – answer T-cell
lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure,
what is the MOST important to document? – answer Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? –
answer Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? –
answer Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen
around the mets, what is the most likely cause? – answer innappropriate slide drying
time
What will help prevent microbial contamination and cross contamination of cultures? –
answer Asceptic technique
, When there are not enough mets to complete the study of a patient/case, what needs to
be done next? – answer prepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? – answer Increading the drying time by using
warm slides
What is true of an inverted microscope? – answer the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - answer DiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose,
wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? – answer Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? – answer Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: - answer
Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important
to monitor to assess the source of the problem? – answer Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase
contrast scope is: - answer Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down
syndrome translocation carrier? – answer 45
What is the best tissue to use for both a good success rate and abnormality detection in
spontaneous abortion miscarriages? – answer Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? –
answer The door does not seal properly
UPDATE LATEST EXAM VERSION MOST
TESTED QUESTIONS WITH VERIFIED
ANSWERS GRADED A+ ASSURED
SUCCESS
What is the best agent to use against mycoplasma? – answer BM cyclin - a combination
of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by: -
answer comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - answer Proteolytic enzymes - break down
tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with: - answer Colcemid - mitotic spindle poison that halts cells at
metaphase
Samples that arrive at the cytogenetics lab should first be: - answer Logged in to the
system
Phytohemagglutinin (PHA) stimulates which type of cells into division? – answer T-cell
lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture failure,
what is the MOST important to document? – answer Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? –
answer Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? –
answer Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen
around the mets, what is the most likely cause? – answer innappropriate slide drying
time
What will help prevent microbial contamination and cross contamination of cultures? –
answer Asceptic technique
, When there are not enough mets to complete the study of a patient/case, what needs to
be done next? – answer prepare additional slides from any remaining sample
When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? – answer Increading the drying time by using
warm slides
What is true of an inverted microscope? – answer the objectives are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - answer DiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose,
wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? – answer Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? – answer Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: - answer
Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important
to monitor to assess the source of the problem? – answer Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a phase
contrast scope is: - answer Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down
syndrome translocation carrier? – answer 45
What is the best tissue to use for both a good success rate and abnormality detection in
spontaneous abortion miscarriages? – answer Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? –
answer The door does not seal properly
