QBM Exam 1 questions with verified answers
UV light Ans✓✓-200-400nm
visible light Ans✓✓-400-800nm
near infrared light Ans✓✓-800-1000nm
DNA + RNA absorption at Ans✓✓-240 nm
Proteins absorb light at Ans✓✓-280 nm
Mohr pipette Ans✓✓-to contain [do not blow out]
dead space below graduation
fill to 1ml mark, empty all = deliver > 1ml
easier to be more accurate with multiple dispenses from single aspiration
serological pipette Ans✓✓-blow out to deliver
graduated to tip, fill to 1 ml mark, empty all = 1 ml delivery
Volumetric pipette Ans✓✓-delivers defined volume accurately
inflexible
Transfer (Pasteur) pieppte Ans✓✓-disposable, uncalibrated
use when precise amount of liquids isn't important
, Theodore Svedberg Ans✓✓-developed analytical ultracentrifugation in early
1900s
1 Svedberg in seconds is Ans✓✓-x10^-13 seconds
relates to sedimentation during centrifugation
centrifugal field is determined by Ans✓✓-square of angular velocity of rotor
radial distance of particle form axis of rotation (r, in cm)
Preparative centrifugation Ans✓✓-most common
allows for actual separation/isolation/purification
large amounts of media/blood/whole cells/organelles/PM/NA/proteins/viruses
morphology, composition, biological activity
analytical centrifugation Ans✓✓-studies sedimentation characteristics of PURE
biological macromolecules + molec. structures
needs only small amounts of materials.
continuous flow centrifuge Ans✓✓-media enters the moving rotor, particles
settle against its wall + excess cleared media overflows through outlet
UV light Ans✓✓-200-400nm
visible light Ans✓✓-400-800nm
near infrared light Ans✓✓-800-1000nm
DNA + RNA absorption at Ans✓✓-240 nm
Proteins absorb light at Ans✓✓-280 nm
Mohr pipette Ans✓✓-to contain [do not blow out]
dead space below graduation
fill to 1ml mark, empty all = deliver > 1ml
easier to be more accurate with multiple dispenses from single aspiration
serological pipette Ans✓✓-blow out to deliver
graduated to tip, fill to 1 ml mark, empty all = 1 ml delivery
Volumetric pipette Ans✓✓-delivers defined volume accurately
inflexible
Transfer (Pasteur) pieppte Ans✓✓-disposable, uncalibrated
use when precise amount of liquids isn't important
, Theodore Svedberg Ans✓✓-developed analytical ultracentrifugation in early
1900s
1 Svedberg in seconds is Ans✓✓-x10^-13 seconds
relates to sedimentation during centrifugation
centrifugal field is determined by Ans✓✓-square of angular velocity of rotor
radial distance of particle form axis of rotation (r, in cm)
Preparative centrifugation Ans✓✓-most common
allows for actual separation/isolation/purification
large amounts of media/blood/whole cells/organelles/PM/NA/proteins/viruses
morphology, composition, biological activity
analytical centrifugation Ans✓✓-studies sedimentation characteristics of PURE
biological macromolecules + molec. structures
needs only small amounts of materials.
continuous flow centrifuge Ans✓✓-media enters the moving rotor, particles
settle against its wall + excess cleared media overflows through outlet
