QBM Lab 6 questions with verified answers
Ensures that the same amount of protein is loaded into each lane Ans✓✓-Sample
normalization
For a more thorough analysis of the purification Ans✓✓-What will normalizing
samplings allow?
SDS-PAGE Ans✓✓-What is used qualitatively after a purification step to
determine if the sample appears more pure?
Compare sample concentration and reaction velocity for quantitative analysis of
the purification scheme Ans✓✓-Purification Table
mg/ml Ans✓✓-Units for protein concentration
Protein concentration (mg/ml) x total volume (ml) = total protein (mg), all
proteins Ans✓✓-Total protein equation
Reaction velocity (units/ml) x total volume (ml) = total activity (units), GST-GFP
only Ans✓✓-Total activity equation
The amount of total protein or GST-GFP at each step of the purification process
Ans✓✓-What does percent yield track?
total activity / total protein = specific activity (units/mg) for GST-GFP Ans✓✓-
Specific activity equation
Ensures that the same amount of protein is loaded into each lane Ans✓✓-Sample
normalization
For a more thorough analysis of the purification Ans✓✓-What will normalizing
samplings allow?
SDS-PAGE Ans✓✓-What is used qualitatively after a purification step to
determine if the sample appears more pure?
Compare sample concentration and reaction velocity for quantitative analysis of
the purification scheme Ans✓✓-Purification Table
mg/ml Ans✓✓-Units for protein concentration
Protein concentration (mg/ml) x total volume (ml) = total protein (mg), all
proteins Ans✓✓-Total protein equation
Reaction velocity (units/ml) x total volume (ml) = total activity (units), GST-GFP
only Ans✓✓-Total activity equation
The amount of total protein or GST-GFP at each step of the purification process
Ans✓✓-What does percent yield track?
total activity / total protein = specific activity (units/mg) for GST-GFP Ans✓✓-
Specific activity equation
