UPDATE LATEST EXAM VERSION MOST
TESTED QUESTIONS WITH VERIFIED
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Whatwiswthewbestwagentwtowusewagainstwmycoplasma?w-wanswerBMwcyclinw-
wawcombinationwofwtwowantibioticsw(tiamulinwandwminocycline)wthatweffecticelywtarge
tswmycoplasmawgrowth
Forwsuccessfulwharvest,weachwnewwbatchwofwhypotonicwsolutionwshouldwbewevaluatedwby:w-
wanswercomparingwthewnewwlotwtowawlotwalreadywinwuse
Colagenase,wpronase,wandwtrypsinware:w-wanswerProteolyticwenzymesw-
wbreakwdownwtissueswintowsingle-cellwsuspensions
Whenwharvestingwperipheralwbloodwcultures,wascepticwtechniquewnowlongerwmatterswfollowi
ngwincubationwwith:w-wanswerColcemidw-
wmitoticwspindlewpoisonwthatwhaltswcellswatwmetaphase
Sampleswthatwarrivewatwthewcytogeneticswlabwshouldwfirstwbe:w-
wanswerLoggedwinwtowthewsystem
Phytohemagglutininw(PHA)wstimulateswwhichwtypewofwcellswintowdivision?w-wanswerT-
cellwlymphocytes
Towavoidwculturewfailure,wmonitoringwreagentswiswcrucial.wWhenwloggingwawculturewfailure,w
whatwiswthewMOSTwimportantwtowdocument?w-
wanswerExpirationwdateswofwreagentswused
WhichwtypewofwmicroscopewiswusedwwhenwcleaningwawCVSw(chorionicwvillus)wsample?w-
wanswerDissection
WhichwcellwtypewiswmostwproblematicwifwfoundwinwawCVSw(chorionicwvillus)wsample?w-
wanswerDeciduaw(maternalwtissue)
Whenwlookingwatwawslidewunderwawphasewcontrastwscopewandwawlotwofwcytoplasmwiswstillwsee
nwaroundwthewmets,wwhatwiswthewmostwlikelywcause?w-
wanswerinnappropriatewslidewdryingwtime
,Whatwwillwhelpwpreventwmicrobialwcontaminationwandwcrosswcontaminationwofwcultures?w-
wanswerAsceptic wtechnique
Whenwtherewarewnotwenoughwmetswtowcompletewthewstudywofwawpatient/case,wwhatwneedswt
owbewdonewnext?w-wanswerpreparewadditionalwslideswfromwanywremainingwsample
Whenwlookingwatwawdroppedwslidewunderwawphasewscopewandwmetswarewverywspreadwoutwan
dwsparse,wwhatwcouldwhelpwfixwthewissue?w-
wanswerIncreadingwthewdryingwtimewbywusingwwarmwslides
Whatwiswtruewofwanwinvertedwmicroscope?w-
wanswerthewobjectiveswarewbeneathwthewstage
High-resolutionwbandingwiswindicatedwforwawpossiblewdiagnosiswof:w-
wanswerDiGenorgewsyndromew--
wmicrodeletionwofw22q11,walsowcalledwvelocardiofacialwsyndrome w
"velo"w-wsoftwpalette,w
"cardio"w-wcongenitalwheartwdefects,w
"facial"w-
wunique wabnormalwfacialwfeatures,wshortwphiltrumw(upperwlip windent),wtubularwnose,wwidewsp
readweyes,wetc
Whenwverifyingwifwawspecimenwiswappropriatewforwcytogeneticwanalysis,wwhatwiswthewMOSTw
importantwdatawtowaccompanywthewspecimen?w-wanswerReasonwforwreferral
Whichwtypewofwspecimenwshouldwbewrequestedwforwawcytogeneticwanalysiswforwturnerwsyndr
ome?w-wanswerHeparinizedwperipheralwbloodwstoredwatwroomwtemperaturew
(NOTwFROZEN)
Awbloodwsamplewcanwbewusedwtowstudywthewcytogeneticswofwawmalignancywonlywif:w-
wanswerDividingwleukemicwcellswarewpresentwinwcirculation
AllwofwthewcultureswinwawCO2wincubatorwfailed,wwhatwaspectswofwthewincubatorwarewimportan
twtowmonitorwtowassesswthewsourcewofwthewproblem?w-
wanswerTemperature,wCO2wlevel,wand
Humiditywlevel
Thewmostwimportantwfactorwaffectingwthewqualitywofwchromosomeswviewedwunderwawphasew
contrastwscopewis:w-wanswerSlidewdryingwafterwthewcellswhavewbeenwdropped
Whatwiswthewmodalwnumberw(defaultwnumberwofwchromosomeswinwawcell)wforwawDownwsyndr
omewtranslocationwcarrier?w-wanswer45
, Whatwiswthewbestwtissuewtowusewforwbothwawgoodwsuccesswratewandwabnormalitywdetectionwi
nwspontaneouswabortionwmiscarriages?w-wanswerChorionicwvilli
Whatwiswawpossiblewcausewforwawdryingwovenwtownotwmaintainwawproperwtemperature?w-
wanswerThewdoorwdoeswnotwsealwproperly
Ifwawtechwwithwawcoldwandwsneezingwdoeswnotwtakewproperwprecautionswwhilewsettingwupwam
nioticwfluidwsamples,wthewspecimenwcouldwbecomewcontaminatedwwith:w-
wanswerMycoplasmaw
--
>wthewmostwcommonwcontaminantwbywawsickwtechwandwcanwbewdetectedwthroughwawspecificw
stainwusedwonwthewsamplew
mycoplasmawcanwinducewabnormalitieswinwawsample,wsowitwiswimportantwtowavoidwcontamin
ation
Whatwaboutwthewmetaphaseswonwawslidewarewaffectedwbywslidewmaking?w-
wanswerCellwdensityw-wthruwdilutionwofwthewsamplewwithwfix
Bandingw-wbasedwonwhowwdark/pale/refractivewtheywarewaffectswtrypsinwtime
Chromosomewspreadingw-waffectedwbywdryingwtime
Whatwtest(s)wshouldwbeworderedwonwawnewbornwwithwsuspectedwPrader-Williwsyndrome?w-
wanswerHighwresolutionwbandingwandwFISHwforwthewPrader-Williwlocuswonw15q11.2
Whenwcapturingwawmetaphase,wawtechwcannotwgetwthewmetwintowfocus,wwhatwiswthewmostwlik
elywcause?w-
wanswerThewcamerawiswnotwparfocalw(inwfocuswwith wthewmicroscpewobjectives) wwith wthewmic
roscope
Unstainedwchromosomewpreparationwshouldwbewviewedwusingwwhichwscope?w-
wanswerPhasewcontrastwcondenser
Thewminimumwbandwlevelwnecessarywtowdetectwprader-williwsyndromewis?w-
wanswer550watwthewlowestw
15q11.2wiswvisiblewatwthatwlevelw
SeewCAPwregulationwCYG.42300
Awregulatorywinspecterwrequestswawcopywofwawlabwpatient'swrecordwforwhiswinspectionwfiles,wt
hewtechwshould:w-wanswerDenywthewinspectorwawcopywofwthewrecord
SeewHIPAAwcompliancewpolicy