anggshay
wednesday 30
5 700pm
Ariananicolemendota
Name__________________________________ TA/Lab Period: many
____________________________________
Lab 10 Handout: Lab Exercise 6-1
2 pts.
10 7
TNTC TNTC TNTC TNTC 33 33
TNTC TNTC 33CFU 2
2 pts.
is sE m s 3.3x10
Practice Problems (Formulas: V1D1=V2D2 and OCD = CFU/original sample volume)
1) You have 0.05 mL of an undiluted culture at a concentration of 3.6 x 106 CFU/mL. You then add 4.95 mL
sterile diluent, what is the dilution, and what is thesD2
final concentration of cells? (2 pts)
- V1 is 0.05 mL
D1 is 3.6 times 10^6
- Dilution can also be concentration is the same thing
- V2 4.95 ml
VIxD1lV2 0.05mL 3.6 106 4.95mL D2
180,000 495mL D2
applyscientific notation D
23138 104CFU ML
wednesday 30
5 700pm
Ariananicolemendota
Name__________________________________ TA/Lab Period: many
____________________________________
Lab 10 Handout: Lab Exercise 6-1
2 pts.
10 7
TNTC TNTC TNTC TNTC 33 33
TNTC TNTC 33CFU 2
2 pts.
is sE m s 3.3x10
Practice Problems (Formulas: V1D1=V2D2 and OCD = CFU/original sample volume)
1) You have 0.05 mL of an undiluted culture at a concentration of 3.6 x 106 CFU/mL. You then add 4.95 mL
sterile diluent, what is the dilution, and what is thesD2
final concentration of cells? (2 pts)
- V1 is 0.05 mL
D1 is 3.6 times 10^6
- Dilution can also be concentration is the same thing
- V2 4.95 ml
VIxD1lV2 0.05mL 3.6 106 4.95mL D2
180,000 495mL D2
applyscientific notation D
23138 104CFU ML
