ASCP CG 2 REAL EXAM QUESTIONS AND CORRECT
ANSWERS 100% VALIDATED FOR THE YEAR 2025.
QUESTION: When harvesting peripheral blood cultures, asceptic technique no longer matters following
incubation with: - ANSWER-Colcemid - mitotic spindle poison that halts cells at metaphase
QUESTION: Samples that arrive at the cytogenetics lab should first be: - ANSWER-Logged in to the
system
QUESTION: Phytohemagglutinin (PHA) stimulates which type of cells into division? - ANSWER-T-cell
lymphocytes
QUESTION: To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what
is the MOST important to document? - ANSWER-Expiration dates of reagents used
What is the best agent to use against mycoplasma? - ANSWER-BM cyclin - a combination of two
antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
QUESTION: For successful harvest, each new batch of hypotonic solution should be evaluated by: -
ANSWER-comparing the new lot to a lot already in use
,QUESTION: Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic enzymes - break down tissues
into single-cell suspensions
QUESTION: Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - ANSWER-
Dissection
QUESTION: Which cell type is most problematic if found in a CVS (chorionic villus) sample? - ANSWER-
Decidua (maternal tissue)
QUESTION: When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen
around the mets, what is the most likely cause? - ANSWER-innappropriate slide drying time
QUESTION: What will help prevent microbial contamination and cross contamination of cultures? -
ANSWER-Asceptic technique
QUESTION: When there are not enough mets to complete the study of a patient/case, what needs to be
done next? - ANSWER-prepare additional slides from any remaining sample
QUESTION: When looking at a dropped slide under a phase scope and mets are very spread out and
sparse, what could help fix the issue? - ANSWER-Increading the drying time by using warm slides
QUESTION: What is true of an inverted microscope? - ANSWER-the objectives are beneath the stage
, QUESTION: High-resolution banding is indicated for a possible diagnosis of: - ANSWER-DiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread
eyes, etc
QUESTION: When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? - ANSWER-Reason for referral
QUESTION: Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? - ANSWER-Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
QUESTION: A blood sample can be used to study the cytogenetics of a malignancy only if: - ANSWER-
Dividing leukemic cells are present in circulation
QUESTION: All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to
monitor to assess the source of the problem? - ANSWER-Temperature,
ANSWERS 100% VALIDATED FOR THE YEAR 2025.
QUESTION: When harvesting peripheral blood cultures, asceptic technique no longer matters following
incubation with: - ANSWER-Colcemid - mitotic spindle poison that halts cells at metaphase
QUESTION: Samples that arrive at the cytogenetics lab should first be: - ANSWER-Logged in to the
system
QUESTION: Phytohemagglutinin (PHA) stimulates which type of cells into division? - ANSWER-T-cell
lymphocytes
QUESTION: To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what
is the MOST important to document? - ANSWER-Expiration dates of reagents used
What is the best agent to use against mycoplasma? - ANSWER-BM cyclin - a combination of two
antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth
QUESTION: For successful harvest, each new batch of hypotonic solution should be evaluated by: -
ANSWER-comparing the new lot to a lot already in use
,QUESTION: Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic enzymes - break down tissues
into single-cell suspensions
QUESTION: Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - ANSWER-
Dissection
QUESTION: Which cell type is most problematic if found in a CVS (chorionic villus) sample? - ANSWER-
Decidua (maternal tissue)
QUESTION: When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen
around the mets, what is the most likely cause? - ANSWER-innappropriate slide drying time
QUESTION: What will help prevent microbial contamination and cross contamination of cultures? -
ANSWER-Asceptic technique
QUESTION: When there are not enough mets to complete the study of a patient/case, what needs to be
done next? - ANSWER-prepare additional slides from any remaining sample
QUESTION: When looking at a dropped slide under a phase scope and mets are very spread out and
sparse, what could help fix the issue? - ANSWER-Increading the drying time by using warm slides
QUESTION: What is true of an inverted microscope? - ANSWER-the objectives are beneath the stage
, QUESTION: High-resolution banding is indicated for a possible diagnosis of: - ANSWER-DiGenorge
syndrome -- microdeletion of 22q11, also called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread
eyes, etc
QUESTION: When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST
important data to accompany the specimen? - ANSWER-Reason for referral
QUESTION: Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? - ANSWER-Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
QUESTION: A blood sample can be used to study the cytogenetics of a malignancy only if: - ANSWER-
Dividing leukemic cells are present in circulation
QUESTION: All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to
monitor to assess the source of the problem? - ANSWER-Temperature,
