CG(ASCP) Exam Review Questions
and Answers 100% Accurate
Pepsin pretreatment - -A method used to enhance the intensity of FISH
probes.
- 2x SSC - -A buffer solution used to improve the intensity of FISH probes.
- Wilm's tumor - -A type of cancer associated with abnormalities in
chromosome 11.
- Ewing sarcoma - -A type of cancer associated with the t(11;22)
translocation.
- Retinoblastoma - -A type of eye cancer associated with abnormalities in
chromosome 13.
- Down Syndrome - -A genetic disorder characterized by low levels of AFP
and estriol, and high levels of HCG.
- Trisomy 18 - -A genetic disorder characterized by low levels of AFP, estriol,
and HCF.
- Slides kept for 3 years - -A guideline from CAP for the retention of slides.
- Prenatal - -Referring to the period of time before birth, usually up to 3
months.
- Quality assurance - -A process to ensure the quality of laboratory results,
different from quality control.
- Gentamycin - -The least toxic antibacterial agent to cells.
- Sodium heparin - -A solution used to transport amniotic fluid at 20C.
- 75% confluent - -The optimal cell culture density for harvest.
- DIRECT harvest - -A method to isolate cytotrophoblast for amino culture.
- No amniocentesis - -Recommended for individuals with a family history of
autosomal recessive disorders.
- Antibacterial agents - -Not necessary for long-term cultures.
, - Ethidium bromide - -Not used as a mitotic arrestant.
- pH - -Does not increase the number of chromosome breaks.
- Blood culture - -Should be performed using a closed system.
- Special culture conditions - -Not required for C bands.
- Sodium chloride - -Not used for hypotonic solutions.
- 2 hours at 90C - -An acceptable duration for slide mounting.
- Mounting medium - -A substance used for slide mounting that affects
refractive index and stain reactivity.
- Same day G band - -A method involving slide incubation at 90C for 1-3
hours and treatment with hydrogen peroxide.
- Temperature of denature solution - -Should be set at 72C.
- 1R1G1F - -An abnormal pattern observed with break-apart probes.
- Array CGH - -The recommended first-tier test for ovarian developmental
delay.
- Parfocal - -Referring to microscope objectives (10x and 100x) that have
the same focal plane.
- Numerical aperture - -The ability of a lens to resolve details.
- Adjust filters - -To control the brightness of the microscope.
- 10x clear, 100x not - -Occurs when two coverslips are placed on a slide or
the slide is upside down.
- Primary factor for mounting medium - -The insolubility of the medium.
- Conventional Giemsa stained chromosomes - -Not suitable for visualizing
translocations.
- Pseudomosaicism - -Can arise from all cell types except fetal tissue cells.
- Chimera - -Referring to a person with two or more different cell lines from
a single zygote.
and Answers 100% Accurate
Pepsin pretreatment - -A method used to enhance the intensity of FISH
probes.
- 2x SSC - -A buffer solution used to improve the intensity of FISH probes.
- Wilm's tumor - -A type of cancer associated with abnormalities in
chromosome 11.
- Ewing sarcoma - -A type of cancer associated with the t(11;22)
translocation.
- Retinoblastoma - -A type of eye cancer associated with abnormalities in
chromosome 13.
- Down Syndrome - -A genetic disorder characterized by low levels of AFP
and estriol, and high levels of HCG.
- Trisomy 18 - -A genetic disorder characterized by low levels of AFP, estriol,
and HCF.
- Slides kept for 3 years - -A guideline from CAP for the retention of slides.
- Prenatal - -Referring to the period of time before birth, usually up to 3
months.
- Quality assurance - -A process to ensure the quality of laboratory results,
different from quality control.
- Gentamycin - -The least toxic antibacterial agent to cells.
- Sodium heparin - -A solution used to transport amniotic fluid at 20C.
- 75% confluent - -The optimal cell culture density for harvest.
- DIRECT harvest - -A method to isolate cytotrophoblast for amino culture.
- No amniocentesis - -Recommended for individuals with a family history of
autosomal recessive disorders.
- Antibacterial agents - -Not necessary for long-term cultures.
, - Ethidium bromide - -Not used as a mitotic arrestant.
- pH - -Does not increase the number of chromosome breaks.
- Blood culture - -Should be performed using a closed system.
- Special culture conditions - -Not required for C bands.
- Sodium chloride - -Not used for hypotonic solutions.
- 2 hours at 90C - -An acceptable duration for slide mounting.
- Mounting medium - -A substance used for slide mounting that affects
refractive index and stain reactivity.
- Same day G band - -A method involving slide incubation at 90C for 1-3
hours and treatment with hydrogen peroxide.
- Temperature of denature solution - -Should be set at 72C.
- 1R1G1F - -An abnormal pattern observed with break-apart probes.
- Array CGH - -The recommended first-tier test for ovarian developmental
delay.
- Parfocal - -Referring to microscope objectives (10x and 100x) that have
the same focal plane.
- Numerical aperture - -The ability of a lens to resolve details.
- Adjust filters - -To control the brightness of the microscope.
- 10x clear, 100x not - -Occurs when two coverslips are placed on a slide or
the slide is upside down.
- Primary factor for mounting medium - -The insolubility of the medium.
- Conventional Giemsa stained chromosomes - -Not suitable for visualizing
translocations.
- Pseudomosaicism - -Can arise from all cell types except fetal tissue cells.
- Chimera - -Referring to a person with two or more different cell lines from
a single zygote.
