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Microbiology Lab Final Exam Questions And
Answers 100% Pass.
Give the steps of a PCR - Answer✔Heat reaction to 95 C to denature DNA
Cool rxn to 55 C to allow primers to anneal
Heat rxn to 72 C to allow Taq polymerase to bind
What machine is used to carry out a PCR reaction? - Answer✔thermocycler
How many cycles does a PCR rxn usually go? - Answer✔30
Name the 9 organisms used in the unknown lab - Answer✔Bacillus subtillis
Enterobacter aerogenes
Entero cloacae
Escherichia coli
Klebsiella pneumoniae
Pseudomonas aeruginosa
Salmonella typhimurium
Staphylococcus aureus
Staphylococcus epidermidis
Which organisms can ferment lactose? - Answer✔E. coli
E. cloacae
E. aerogenes
K. pneumonia
Define caliform bacteria - Answer✔rod-shaped, Gram-negative, non-spore forming, and motile
or non-motile bacteria which can ferment lactose with the production of acid and gas
Name the selective medias used in class - Answer✔Blood Agar
Bile Esculin
, © EVERLY 2025 ALL RIGHTS RESERVED.
Mannitol Salt Agar (MSA)
MacConkey Agar (MaC)
Eosin Methylene Blue (EMB)
Hektoen Enteric Agar (HEA)
Xylose Lysine Desoxiolate (XLD)
Name the differential medias used in class - Answer✔O/F Glucose
MRVP
Catalase
Oxidase
Citrate
Starch
Indole
TSI
What does the acronym IMViC stand for? - Answer✔indole
methyl red
vogues proskaver
in
citrate
What is the IMViC series of tests used for? - Answer✔used to differentiate gram negative rods
Which specific orgnaisms are the IMViC tests good at differentiating? - Answer✔Enterobacter
Define selective media - Answer✔designed to grow only specific bacteria but not others
Define differential media - Answer✔designed to show the difference between different
organisms grown on
What does blood agar show? - Answer✔shows the types of hemolysis (ability to break down
RBCs) of organisms
What are the 3 types of hemolysis? - Answer✔alpha
beta
gamma
Describe alpha growth on blood agar - Answer✔partial growth, green
Microbiology Lab Final Exam Questions And
Answers 100% Pass.
Give the steps of a PCR - Answer✔Heat reaction to 95 C to denature DNA
Cool rxn to 55 C to allow primers to anneal
Heat rxn to 72 C to allow Taq polymerase to bind
What machine is used to carry out a PCR reaction? - Answer✔thermocycler
How many cycles does a PCR rxn usually go? - Answer✔30
Name the 9 organisms used in the unknown lab - Answer✔Bacillus subtillis
Enterobacter aerogenes
Entero cloacae
Escherichia coli
Klebsiella pneumoniae
Pseudomonas aeruginosa
Salmonella typhimurium
Staphylococcus aureus
Staphylococcus epidermidis
Which organisms can ferment lactose? - Answer✔E. coli
E. cloacae
E. aerogenes
K. pneumonia
Define caliform bacteria - Answer✔rod-shaped, Gram-negative, non-spore forming, and motile
or non-motile bacteria which can ferment lactose with the production of acid and gas
Name the selective medias used in class - Answer✔Blood Agar
Bile Esculin
, © EVERLY 2025 ALL RIGHTS RESERVED.
Mannitol Salt Agar (MSA)
MacConkey Agar (MaC)
Eosin Methylene Blue (EMB)
Hektoen Enteric Agar (HEA)
Xylose Lysine Desoxiolate (XLD)
Name the differential medias used in class - Answer✔O/F Glucose
MRVP
Catalase
Oxidase
Citrate
Starch
Indole
TSI
What does the acronym IMViC stand for? - Answer✔indole
methyl red
vogues proskaver
in
citrate
What is the IMViC series of tests used for? - Answer✔used to differentiate gram negative rods
Which specific orgnaisms are the IMViC tests good at differentiating? - Answer✔Enterobacter
Define selective media - Answer✔designed to grow only specific bacteria but not others
Define differential media - Answer✔designed to show the difference between different
organisms grown on
What does blood agar show? - Answer✔shows the types of hemolysis (ability to break down
RBCs) of organisms
What are the 3 types of hemolysis? - Answer✔alpha
beta
gamma
Describe alpha growth on blood agar - Answer✔partial growth, green
