Microbiology lab Practical Exam 1 Questions
And Answers 100% Pass.
Wet mount steps: - Answer✔1.water
2. specimen
3. Cover slip over specimen
hanging drop steps: - Answer✔1. Vaseline around cover slip
2. micro organism in center of cover slip
3. place concave slide on cover slip
Gram stain steps: - Answer✔1. Crystal Violet
2. Gram's Iodine
3. Ethyl Alcohol
4. Safranin Red
negative stain steps: - Answer✔1. nigrosin
2.heat loop
3.bacteria
4. slide to spread mixture
5. air dry
acid-fast stain steps: - Answer✔1. carbolfuchsin
2. acid alcohol
3. methylene blue
4. wash & dry
simple stain steps - Answer✔1. fixed smear slide
2. dyes
3. rinse & dry
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nutrient broth - Answer✔-liquid media used to grow bacteria
agar - Answer✔-used to culture microorganisms
candle jar - Answer✔-used for anaerobic organisms
Petri dish - Answer✔-Used to culture or observe bacteria growth
slant - Answer✔-a medium for which bacteria can grow on.
E.coli Gram stain - Answer✔red colored rods
S. epidermidis Gram stain - Answer✔purple colored grapes
B. subtilis Gram stain - Answer✔purple colored rods
list information gained from a
particular slide based on staining techniques (cell morphology, arrangement, cell wall structure,
Gram stain reaction or other staining reaction). - Answer✔...
show motility on a hanging drop - Answer✔movement because of flagella
show motility on a Brownian movement. - Answer✔bacteria moves randomly, due to the energy
of a system (vibration)
why does motility occur in bacteria ? - Answer✔they have a flagellum that propels them around.
why does motility occur in protozoans? - Answer✔1. Flagella 2. Cilia 3. Amoeba
aseptic technique steps - Answer✔1. flame loop
2. flame tube
3. inoculate bacteria
4. flame loop
5. flame tube
solid culture set up - Answer✔1- water
2-heat loop
3-bacteria
4-air dry
5-apply heat
broth culture set up - Answer✔1. heat loop
2. bacteria
3. air dry