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Microbiology Lab Exam 1 Questions And
Answers 100% Pass.
When viewing a smear with the oil immersion lens the final magnification is -
Answer✔1000x;10x ocular x 100X objective
what is the purpose of heat fixing - Answer✔kill the bacteria, adhere to glass anddenature the
proteins of bacteria
name oneof the stains or regents used in the gram stain procedure - Answer✔gram crystal violet,
gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - Answer✔to ensure that we do not contaminate what
we are working with as as to ensue that we do not acciently inoculate ouselves. example of
aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming the test tube before
and after obtaining the sample.
the gram reaction is due to differences in the structure of wat part of the microbe - Answer✔its
cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer
compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab - Answer✔loop, needle
what color is a gram positive microbe - Answer✔purple
waht is the function of the ethanol in the gram stain procedure - Answer✔decolorize
name one of the strains in the acid fast stain - Answer✔cold kinyoun, carbol fuschsin, methylen
blue
name one of the stains in the endospore stain - Answer✔malachite green, safranin
what is the purpose of the acid-fast stain - Answer✔the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain - Answer✔red
what is the function of agarin the media - Answer✔agar allows media to remain solid at room
temperature
what is the difference between broth and agar based media - Answer✔broth is liquid media at
room temperature, agar media is solid at room temp.
Microbiology Lab Exam 1 Questions And
Answers 100% Pass.
When viewing a smear with the oil immersion lens the final magnification is -
Answer✔1000x;10x ocular x 100X objective
what is the purpose of heat fixing - Answer✔kill the bacteria, adhere to glass anddenature the
proteins of bacteria
name oneof the stains or regents used in the gram stain procedure - Answer✔gram crystal violet,
gram iodine, gram sfaranin, 95% ethanol
why are aseptic techniques used in the lab? - Answer✔to ensure that we do not contaminate what
we are working with as as to ensue that we do not acciently inoculate ouselves. example of
aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming the test tube before
and after obtaining the sample.
the gram reaction is due to differences in the structure of wat part of the microbe - Answer✔its
cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer
compared to GN bacteria.
Name 2 inoculating tools you will be using in this lab - Answer✔loop, needle
what color is a gram positive microbe - Answer✔purple
waht is the function of the ethanol in the gram stain procedure - Answer✔decolorize
name one of the strains in the acid fast stain - Answer✔cold kinyoun, carbol fuschsin, methylen
blue
name one of the stains in the endospore stain - Answer✔malachite green, safranin
what is the purpose of the acid-fast stain - Answer✔the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
what color does na acid-fast microbe stain with acd-fast stain - Answer✔red
what is the function of agarin the media - Answer✔agar allows media to remain solid at room
temperature
what is the difference between broth and agar based media - Answer✔broth is liquid media at
room temperature, agar media is solid at room temp.
