1|Page
Microbiology Lab Final Exam Questions and
Answers 100% Pass
Give the steps of a PCR - ✔✔Heat reaction to 95 C to denature DNA
Cool rxn to 55 C to allow primers to anneal
Heat rxn to 72 C to allow Taq polymerase to bind
What machine is used to carry out a PCR reaction? - ✔✔thermocycler
How many cycles does a PCR rxn usually go? - ✔✔30
Name the 9 organisms used in the unknown lab - ✔✔Bacillus subtillis
Enterobacter aerogenes
Entero cloacae
Escherichia coli
Klebsiella pneumoniae
Pseudomonas aeruginosa
Salmonella typhimurium
Staphylococcus aureus
Staphylococcus epidermidis
Which organisms can ferment lactose? - ✔✔E. coli
E. cloacae
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
, 2|Page
E. aerogenes
K. pneumonia
Define caliform bacteria - ✔✔rod-shaped, Gram-negative, non-spore forming, and motile or non-motile
bacteria which can ferment lactose with the production of acid and gas
Name the selective medias used in class - ✔✔Blood Agar
Bile Esculin
Mannitol Salt Agar (MSA)
MacConkey Agar (MaC)
Eosin Methylene Blue (EMB)
Hektoen Enteric Agar (HEA)
Xylose Lysine Desoxiolate (XLD)
Name the differential medias used in class - ✔✔O/F Glucose
MRVP
Catalase
Oxidase
Citrate
Starch
Indole
TSI
What does the acronym IMViC stand for? - ✔✔indole
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
Microbiology Lab Final Exam Questions and
Answers 100% Pass
Give the steps of a PCR - ✔✔Heat reaction to 95 C to denature DNA
Cool rxn to 55 C to allow primers to anneal
Heat rxn to 72 C to allow Taq polymerase to bind
What machine is used to carry out a PCR reaction? - ✔✔thermocycler
How many cycles does a PCR rxn usually go? - ✔✔30
Name the 9 organisms used in the unknown lab - ✔✔Bacillus subtillis
Enterobacter aerogenes
Entero cloacae
Escherichia coli
Klebsiella pneumoniae
Pseudomonas aeruginosa
Salmonella typhimurium
Staphylococcus aureus
Staphylococcus epidermidis
Which organisms can ferment lactose? - ✔✔E. coli
E. cloacae
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
, 2|Page
E. aerogenes
K. pneumonia
Define caliform bacteria - ✔✔rod-shaped, Gram-negative, non-spore forming, and motile or non-motile
bacteria which can ferment lactose with the production of acid and gas
Name the selective medias used in class - ✔✔Blood Agar
Bile Esculin
Mannitol Salt Agar (MSA)
MacConkey Agar (MaC)
Eosin Methylene Blue (EMB)
Hektoen Enteric Agar (HEA)
Xylose Lysine Desoxiolate (XLD)
Name the differential medias used in class - ✔✔O/F Glucose
MRVP
Catalase
Oxidase
Citrate
Starch
Indole
TSI
What does the acronym IMViC stand for? - ✔✔indole
Created by Katelyn Whitman © 2025, All Rights Reserved. 100% PASS GUARANTEE
