ACTUAL Questions and CORRECT
Answers
During tissue processing, what is the correct sequence of steps ?
a) clearing, dehydration, infiltration
b) clearing, infiltration, dehydration
c) dehydration, infiltration, clearing
d) dehydration, clearing, infiltration - CORRECT ANSWER - d) dehydration, clearing,
infiltration
If a person has A- blood, who can they receive from within the ABO blood group? - CORRECT
ANSWER - A-, AB-
What is the purpose of fixation? - CORRECT ANSWER - To stabilize proteins & colloidal
material in cells/tissues so that it becomes resistant to change/remains as lifelike as possible
Why is pure water neutral on the pH scale? - CORRECT ANSWER - Water has equal
amounts of hydrogen and hydroxide atoms, therefore it is able to self ionize.
How can the formation of paraformaldehyde be avoided - CORRECT ANSWER -
Additives to formalin such as buffers or methanol
What tube would be used to draw blood from a patient being tested for hematocrit? - CORRECT
ANSWER - Lavender
What is a compound fixative? - CORRECT ANSWER - Composed of 2 or more primary
fixatives.
,What is the normal pH of blood? - CORRECT ANSWER - 7.35-7.45
How many stool samples must be collected for an ova & parasites exam? Why? - CORRECT
ANSWER - 3 different samples from 3 different days because parasites can appear
sporadically.
What is serum used for in culture media? - CORRECT ANSWER - Can either enrich or
solidify media.
What is inspissation? - CORRECT ANSWER - The process of denaturing & coagulating
serum by heating to 85 Celsius
What is basic (nutrient) media? - CORRECT ANSWER - Contains common nutritional
ingredients to support the growth of non-fastidious organisms. Not normally plate media.
What primary basic ingredient provides a carbon & energy source for bacteria growth? -
CORRECT ANSWER - Carbohydrates (glucose)
Appromixmately how much blood is in a human adult body? - CORRECT ANSWER - 5L
What is the most common stain used for cytology testing? - CORRECT ANSWER - PAP
smear (Papinicolaou's staining technique)
What are the four basic types of tissue? - CORRECT ANSWER - Epithelial, connective,
muscular, nervous
A movement that takes a body part further away from it's central axis is called a(n): - CORRECT
ANSWER - Abduction
Incomplete, partial hemolysis around bacterial colonies on a growth plate is called:
, a) Alpha hemolysis
b) Beta hemolysis
c) Gamma hemolysis - CORRECT ANSWER - a) Alpha hemolysis
What antiseptic is used to clean the venipuncture site? - CORRECT ANSWER - 70%
isopropyl alcohol
Where are red blood cells produced in a fetus? - CORRECT ANSWER - The liver
What does mean measure? - CORRECT ANSWER - The average value of numbers within
a set
What is a chromogen? - CORRECT ANSWER - Added to media as an indicator by
changing the colour of a bacterial colony.
What colour would a Gram positive be? - CORRECT ANSWER - purple-blue
What is a buffer system? - CORRECT ANSWER - Helps to maintain a relatively constant
pH. Chemical compounds that covert strong acids and bases into weak ones.
What dye is used as the primary stain when performing a Gram Stain? - CORRECT
ANSWER - Crystal/Gentian Violet
What is a blastoconidia? - CORRECT ANSWER - Buds/ 'daughter cells' of yeasts. They
can extend to form a chain-like structure called pseudohyphae.
What is added to MacConkey plate media to make it selective? - CORRECT ANSWER -
Crystal violet and bile salts inhibit the growth of gram positive bacteria