GMS 5630 - Medical Histology - Test 1
questions well answered to pass
Histology is the study of? - correct answer ✔✔Cells and tissues
What are the three main components of a cell? - correct answer ✔✔Nucleus
Plasma Membrane
Cytoplasm
Compartmentalization describes what in regard to cell function? - correct answer ✔✔Division of
function among various organelles with varying functions.
Fixation - correct answer ✔✔fixing cells and tissues into place on the microscope slide.
Fixative agents used in light microscopy - correct answer ✔✔Formaldehyde and Alcohol
Fixative agents used in EM - correct answer ✔✔Glutaraldehyde and Osmium Tetroxide
Dehydration agents Light Microscopy - correct answer ✔✔Alcohol and Acetone
Dehydration agents EM - correct answer ✔✔Alcohol and Acetone
Infiltration and Embedding agents used for light microscopy - correct answer ✔✔Paraffin and Plastic
Infiltration and Embedding agents used for EM - correct answer ✔✔Plastic
Which embedding agent allows for thinner sections to be obtained from the histological slide? - correct
answer ✔✔Plastic
,Most common Light microscopy stain? - correct answer ✔✔Dyes (H&E)
Staining for EM - correct answer ✔✔Heavy Metals (Pb)
Basophilic means - correct answer ✔✔"base liking"
Molecules that are attracted to basic dyes with positive charges such as acidic or negatively charged
molecules.
Basophilic structures of the cell - correct answer ✔✔Nucleic acids
DNA, RNA, Ribosomes, RER
Basophilic structures will be what color with H&E stain? - correct answer ✔✔Blue
Acidophilic means? - correct answer ✔✔"Acid liking"
Molecules that are positively charged and attracted to the negative charge of an acidic stain.
Acidophilic structures in the cell - correct answer ✔✔Proteins
Acidophilic structures will stain what color with H&E? - correct answer ✔✔Pink
Toluidine Blue stains what structures? - correct answer ✔✔Nucleic acids blue and glycogen magenta
What stain will stain collagen fibers green/blue? - correct answer ✔✔Masson Trichrome
What stain will turn elastic fibers black? - correct answer ✔✔Verhoeff's Hematoxylin
How does PAS stain work? - correct answer ✔✔Periodic Acid will react with hydroxyl group on
carbohydrates creating aldehyde. Schiff interacts with the aldehyde causing the magenta color.
,What molecules do not stain well with H&E? - correct answer ✔✔Glycogen
What type of immunofluorescence enhances the signal of the probe? - correct answer ✔✔Indirect
Resolution - correct answer ✔✔The distance between resolvable points.
Membrane-bound organelles - correct answer ✔✔PM
Nucleus
Mitochondria
Lysosomes
Endosomes
SER, RER
Golgi Apparatus
Non-membrane bound organelles - correct answer ✔✔Cytosol
Nucleolus
Centrioles
Cytoskeleton
Ribosomes
Proteosome
What is the difference between integral and peripheral proteins on the PM? - correct answer
✔✔Integral proteins span the membrane at least halfway or fully (transmembrane). Peripheral proteins
are just on the surface of the PM.
What is a glycoprotein - correct answer ✔✔carbohydrate attached to a protein
The PM is made from what type of molecule - correct answer ✔✔Amphiphilic phospholipids
, What are the functions of the PM - correct answer ✔✔- Ion and nutrient transport
- Recognition of environmental signals
- Adhesion to cells and ECM
- Endocytosis
- Secretion (exocytosis)
What are the various types of proteins that can be found in the PM? - correct answer ✔✔Pumps,
channels, receptors, linkers, enzymes, structural proteins.
Structural proteins are responsible for - correct answer ✔✔Anchoring cells together
What types of molecules can pass through the PM via simple diffusion? - correct answer ✔✔Small
noncharged and hydrophobic molecules and gasses (CO2, O2)
How are carrier and channel proteins different? - correct answer ✔✔Carriers can go up or down the
gradient while channel proteins can only go down the gradient.
What are some types of channel proteins? - correct answer ✔✔Voltage gated, enzymatic, mechanical
What are the four types of carrier proteins? - correct answer ✔✔Uniporter
Symporter
Antiporter
Pump
What is the only cell that has no nucleus? - correct answer ✔✔RBC
What organelle stores DNA? - correct answer ✔✔Nucleus
Which part of the nucleus synthesizes RNA? - correct answer ✔✔Nucleolus
questions well answered to pass
Histology is the study of? - correct answer ✔✔Cells and tissues
What are the three main components of a cell? - correct answer ✔✔Nucleus
Plasma Membrane
Cytoplasm
Compartmentalization describes what in regard to cell function? - correct answer ✔✔Division of
function among various organelles with varying functions.
Fixation - correct answer ✔✔fixing cells and tissues into place on the microscope slide.
Fixative agents used in light microscopy - correct answer ✔✔Formaldehyde and Alcohol
Fixative agents used in EM - correct answer ✔✔Glutaraldehyde and Osmium Tetroxide
Dehydration agents Light Microscopy - correct answer ✔✔Alcohol and Acetone
Dehydration agents EM - correct answer ✔✔Alcohol and Acetone
Infiltration and Embedding agents used for light microscopy - correct answer ✔✔Paraffin and Plastic
Infiltration and Embedding agents used for EM - correct answer ✔✔Plastic
Which embedding agent allows for thinner sections to be obtained from the histological slide? - correct
answer ✔✔Plastic
,Most common Light microscopy stain? - correct answer ✔✔Dyes (H&E)
Staining for EM - correct answer ✔✔Heavy Metals (Pb)
Basophilic means - correct answer ✔✔"base liking"
Molecules that are attracted to basic dyes with positive charges such as acidic or negatively charged
molecules.
Basophilic structures of the cell - correct answer ✔✔Nucleic acids
DNA, RNA, Ribosomes, RER
Basophilic structures will be what color with H&E stain? - correct answer ✔✔Blue
Acidophilic means? - correct answer ✔✔"Acid liking"
Molecules that are positively charged and attracted to the negative charge of an acidic stain.
Acidophilic structures in the cell - correct answer ✔✔Proteins
Acidophilic structures will stain what color with H&E? - correct answer ✔✔Pink
Toluidine Blue stains what structures? - correct answer ✔✔Nucleic acids blue and glycogen magenta
What stain will stain collagen fibers green/blue? - correct answer ✔✔Masson Trichrome
What stain will turn elastic fibers black? - correct answer ✔✔Verhoeff's Hematoxylin
How does PAS stain work? - correct answer ✔✔Periodic Acid will react with hydroxyl group on
carbohydrates creating aldehyde. Schiff interacts with the aldehyde causing the magenta color.
,What molecules do not stain well with H&E? - correct answer ✔✔Glycogen
What type of immunofluorescence enhances the signal of the probe? - correct answer ✔✔Indirect
Resolution - correct answer ✔✔The distance between resolvable points.
Membrane-bound organelles - correct answer ✔✔PM
Nucleus
Mitochondria
Lysosomes
Endosomes
SER, RER
Golgi Apparatus
Non-membrane bound organelles - correct answer ✔✔Cytosol
Nucleolus
Centrioles
Cytoskeleton
Ribosomes
Proteosome
What is the difference between integral and peripheral proteins on the PM? - correct answer
✔✔Integral proteins span the membrane at least halfway or fully (transmembrane). Peripheral proteins
are just on the surface of the PM.
What is a glycoprotein - correct answer ✔✔carbohydrate attached to a protein
The PM is made from what type of molecule - correct answer ✔✔Amphiphilic phospholipids
, What are the functions of the PM - correct answer ✔✔- Ion and nutrient transport
- Recognition of environmental signals
- Adhesion to cells and ECM
- Endocytosis
- Secretion (exocytosis)
What are the various types of proteins that can be found in the PM? - correct answer ✔✔Pumps,
channels, receptors, linkers, enzymes, structural proteins.
Structural proteins are responsible for - correct answer ✔✔Anchoring cells together
What types of molecules can pass through the PM via simple diffusion? - correct answer ✔✔Small
noncharged and hydrophobic molecules and gasses (CO2, O2)
How are carrier and channel proteins different? - correct answer ✔✔Carriers can go up or down the
gradient while channel proteins can only go down the gradient.
What are some types of channel proteins? - correct answer ✔✔Voltage gated, enzymatic, mechanical
What are the four types of carrier proteins? - correct answer ✔✔Uniporter
Symporter
Antiporter
Pump
What is the only cell that has no nucleus? - correct answer ✔✔RBC
What organelle stores DNA? - correct answer ✔✔Nucleus
Which part of the nucleus synthesizes RNA? - correct answer ✔✔Nucleolus
