Micro 2460 UTA AOBSN Lab Practical 1 (Labs 1, 2, 3, 4)
Questions With Complete Solutions
absorbance of light Correct Answers spectrophotometry
measures cell density (turbidity) via light absorbtion of cells
more tubidity=more bacteria present
absorbance is directly proportional to cell count
Acid Fast Stain Correct Answers differential stain
mycolic acid cause waxy cell wall
carbolfuchsin, water
decolorizer, water
methylene blue, water
blot
microscope
acid fast-red
non-acid-fast-blue
acid stain Correct Answers negatively charged chromosphere
repells negatively charged bacteria
dyes background
aeptic techniques Correct Answers without contamination
handling microbes and materials to minimize contamination
Aperture diaphragm control Correct Answers controls amount
of light entering condenser
1/2 open for 10x and 40x
completely open-oil immersion
too much light bleaches out specimen
, Arm Correct Answers Supports nose piece
Holds power switch
Holds focus adjustment knobs
Bacillus Correct Answers rod or oval shaped
Base Correct Answers holds illuminator
supports the rest of the microscope
Basic stain Correct Answers positively charged chromosphere
attaches to negatively charged bacteria cells
dyes the cell
Body Correct Answers Holds mirrors that bend light into
oculars
images reversed and inverted
Capsule Stain Correct Answers Indirect simple stain
nigrosin
add bacteria, smear
air dry
crystal violet, water
blot
microscope
background is dyed, capsules are not
coccus Correct Answers spherical shape
Colony Forming Units Correct Answers CFUs
counts of colonies on surface in plate count method
only living cells grow
Questions With Complete Solutions
absorbance of light Correct Answers spectrophotometry
measures cell density (turbidity) via light absorbtion of cells
more tubidity=more bacteria present
absorbance is directly proportional to cell count
Acid Fast Stain Correct Answers differential stain
mycolic acid cause waxy cell wall
carbolfuchsin, water
decolorizer, water
methylene blue, water
blot
microscope
acid fast-red
non-acid-fast-blue
acid stain Correct Answers negatively charged chromosphere
repells negatively charged bacteria
dyes background
aeptic techniques Correct Answers without contamination
handling microbes and materials to minimize contamination
Aperture diaphragm control Correct Answers controls amount
of light entering condenser
1/2 open for 10x and 40x
completely open-oil immersion
too much light bleaches out specimen
, Arm Correct Answers Supports nose piece
Holds power switch
Holds focus adjustment knobs
Bacillus Correct Answers rod or oval shaped
Base Correct Answers holds illuminator
supports the rest of the microscope
Basic stain Correct Answers positively charged chromosphere
attaches to negatively charged bacteria cells
dyes the cell
Body Correct Answers Holds mirrors that bend light into
oculars
images reversed and inverted
Capsule Stain Correct Answers Indirect simple stain
nigrosin
add bacteria, smear
air dry
crystal violet, water
blot
microscope
background is dyed, capsules are not
coccus Correct Answers spherical shape
Colony Forming Units Correct Answers CFUs
counts of colonies on surface in plate count method
only living cells grow
