Bio 150 Lab EXAM 2 Question and
answers 100% correct (10 pgs)
What does the pH scale measure? What makes pure water neutral on that
scale? - correct answer ✔-pH is determined by the amount of H+ ions and
OH- ions that appear in an aqueous solution.
-Scale is 1 to 14, with 1 contianing the most hydrogen ions and 14 having the
least. 1-6 is acidic. 7 is neutral, and 8-14 is known as basic.
-Pure water is neutral because the number of positive hydrogen ions
produced is equal to the number of negative.
What is the importance of enzymes in living system? - correct answer ✔an
enzyme is a protein that acts as a catalyst speeding up reactions, and reduce
activation energy. Without enzymes the reactions in the body would be so
slow that we would cease to live, and without enzymes cell reactions would
require too much energy that our body is not able to provide.
What is the optimum pH of an enzyme? Why is the pH of the environment of
an enzymatic reaction important? - correct answer ✔6-8 or depends on what
type of environment its in.When the pH value becomes very high or too low,
the basic structure of the enzyme undergoes changes. As a result, the active
site of the enzyme fails to bind well with the substrate properly and the activity
of the enzyme gets badly affected. The enzyme may even stop functioning
completely.
What is the optimum temperature of an enzyme? - correct answer ✔optimal
temperature is usually around human body temperature (37.5 C)
, Do you think high fever for long period of time would alter cellular function?
Explain your answer. - correct answer ✔yes, If the temperature reaches a
high point like 40-41 celsius, many of the proteins will reach its "flocculation"
point, and suffer denaturation or precipitation
Every enzyme has its specific substrate. What determines this specificity of an
enzyme? - correct answer ✔The structure of the enzyme determines which
substrates it interacts with
Enzymes increase the rate of a reaction by - correct answer ✔decreasing the
activation energy needed to start the reaction
Do you expect an enzyme to be active at 0oC. Why - correct answer ✔Yes,
the enzyme is
still active but is working at slower rates.
If you keep an enzyme at 0 C for an extended period of time and then bring
back to its optimum temperature, would you expect the enzyme to be active?
Explain - correct answer ✔Yes, because the enzyme is not denatured at 0o
C, it just works at a slow rate, but when your increase the temperature to its
optimum temperature, the enzyme will work.
Why was hydrolysis of starch by human amylase, in your enzyme lab
experiment, very slow (or failed to happen) at 85oC - correct answer ✔The
enzyme (amylase) at this point was denatured, so there was no enzyme to
work on the starch, so less product was produced.
Why was hydrolysis of starch by bacterial amylase, in your enzyme lab
experiment, faster at 85oC? - correct answer ✔......
answers 100% correct (10 pgs)
What does the pH scale measure? What makes pure water neutral on that
scale? - correct answer ✔-pH is determined by the amount of H+ ions and
OH- ions that appear in an aqueous solution.
-Scale is 1 to 14, with 1 contianing the most hydrogen ions and 14 having the
least. 1-6 is acidic. 7 is neutral, and 8-14 is known as basic.
-Pure water is neutral because the number of positive hydrogen ions
produced is equal to the number of negative.
What is the importance of enzymes in living system? - correct answer ✔an
enzyme is a protein that acts as a catalyst speeding up reactions, and reduce
activation energy. Without enzymes the reactions in the body would be so
slow that we would cease to live, and without enzymes cell reactions would
require too much energy that our body is not able to provide.
What is the optimum pH of an enzyme? Why is the pH of the environment of
an enzymatic reaction important? - correct answer ✔6-8 or depends on what
type of environment its in.When the pH value becomes very high or too low,
the basic structure of the enzyme undergoes changes. As a result, the active
site of the enzyme fails to bind well with the substrate properly and the activity
of the enzyme gets badly affected. The enzyme may even stop functioning
completely.
What is the optimum temperature of an enzyme? - correct answer ✔optimal
temperature is usually around human body temperature (37.5 C)
, Do you think high fever for long period of time would alter cellular function?
Explain your answer. - correct answer ✔yes, If the temperature reaches a
high point like 40-41 celsius, many of the proteins will reach its "flocculation"
point, and suffer denaturation or precipitation
Every enzyme has its specific substrate. What determines this specificity of an
enzyme? - correct answer ✔The structure of the enzyme determines which
substrates it interacts with
Enzymes increase the rate of a reaction by - correct answer ✔decreasing the
activation energy needed to start the reaction
Do you expect an enzyme to be active at 0oC. Why - correct answer ✔Yes,
the enzyme is
still active but is working at slower rates.
If you keep an enzyme at 0 C for an extended period of time and then bring
back to its optimum temperature, would you expect the enzyme to be active?
Explain - correct answer ✔Yes, because the enzyme is not denatured at 0o
C, it just works at a slow rate, but when your increase the temperature to its
optimum temperature, the enzyme will work.
Why was hydrolysis of starch by human amylase, in your enzyme lab
experiment, very slow (or failed to happen) at 85oC - correct answer ✔The
enzyme (amylase) at this point was denatured, so there was no enzyme to
work on the starch, so less product was produced.
Why was hydrolysis of starch by bacterial amylase, in your enzyme lab
experiment, faster at 85oC? - correct answer ✔......
