Anatomy and Physiology
Lab 3: The Microscope
Learning Outcomes
1. Identify the parts of the microscope, and list the function of each
2. Describe and demonstrate the proper techniques for care of the microscope
3. Define total resolution, parfocal, field, depth of field, working distance, and magnification
4. Measure the field diameter for One objective lens, calculate It for all the other objective
lenses, and estimate the size of objects in each
5. Discuss the general relationships between magnificaton, working distance, and fieId diameter
Care and Structure of the Compound Microscope
When transporting the microscope, hold it in an upright positon, with one hand on its arm
and other supporting it's base.
Use only special grit-free lense paper.
Always begin process with the scanning objective lens in position, changing to the higher-
power lenses as necessary
Use the coarse adjustment knob only with the scanning objective lens.
Always use a cover slip with wet mount preparation.
Before putting the microscope in storage cabinet, remove the slide from the Stage, rotate in
the scanning objective lens into position, wrap the cord as directed, and replaced the dust
cover or return the microscope to the appropriate storage area.
Never remove any parts from the microscope.
Parts of a Microscope
Ocular lenses
Diopter Adjustment Knob
Rotating Nosepiece
Arm Objective lens
Slide Holder
Stage
Condenser
Coarse Adjustment Knob
Iris Diagram
Fine Adjustment Knob Substance Light
Base
Light Control
Lab 3: The Microscope
Learning Outcomes
1. Identify the parts of the microscope, and list the function of each
2. Describe and demonstrate the proper techniques for care of the microscope
3. Define total resolution, parfocal, field, depth of field, working distance, and magnification
4. Measure the field diameter for One objective lens, calculate It for all the other objective
lenses, and estimate the size of objects in each
5. Discuss the general relationships between magnificaton, working distance, and fieId diameter
Care and Structure of the Compound Microscope
When transporting the microscope, hold it in an upright positon, with one hand on its arm
and other supporting it's base.
Use only special grit-free lense paper.
Always begin process with the scanning objective lens in position, changing to the higher-
power lenses as necessary
Use the coarse adjustment knob only with the scanning objective lens.
Always use a cover slip with wet mount preparation.
Before putting the microscope in storage cabinet, remove the slide from the Stage, rotate in
the scanning objective lens into position, wrap the cord as directed, and replaced the dust
cover or return the microscope to the appropriate storage area.
Never remove any parts from the microscope.
Parts of a Microscope
Ocular lenses
Diopter Adjustment Knob
Rotating Nosepiece
Arm Objective lens
Slide Holder
Stage
Condenser
Coarse Adjustment Knob
Iris Diagram
Fine Adjustment Knob Substance Light
Base
Light Control
