BCH 461 Final Exam questions well answered to pass

BCH 461 Final Exam

Most transcription factors that bind to DNA are dimers (T/F) - correct answer ✔✔True



The Lucine zipper is an Example of a DNA binding Motif in the minor Grove (T/F) - correct answer
✔✔False (major groove)



DNA Almost always binds in the major groove (T/F) - correct answer ✔✔True



In bacteria, a sequence at the N-terminus is recognized by a secreatin system resulting in translocation of
protein out of cytosol (T/F) - correct answer ✔✔True



Silencing mRNA requires production of double stranded RNA (T/F) - correct answer ✔✔True
(intermediate)



Steroid hormone receptors repress transcription genes when bound to their steroid hormone (T/F) -
correct answer ✔✔False (Activate)



Regulation of expression by low iron involves 3 things - correct answer ✔✔1. IRP1 loses Iron sulfer
cluster

2. IRP1 binds to IRE

3. The binding occurs in the 3' untranslated version of mRNA



3 facts about proofreading by amino acyl-tRNA - correct answer ✔✔1. can result in hydrolysis of
adenylated amino acid

2. can result in hydrolysis of amino acyl-tRNA

3. specific for incorrect amino acids



Double stranded RNA formes a silencing complex by .... - correct answer ✔✔-Processessing by Drosha
RNA endonuclease in the nucleas to release RNA hairpins.

, -Professing by Dicer RNA endonuclease in cytosol

-Unwinding of Strands by RNA helicase

-Incorrperation of a strand into the RISC



The amino acid stem is attached to the amino acid (T/F) - correct answer ✔✔True



Bases in the T(sci)C loop are H-bonded to bases in the D-loop - correct answer ✔✔True



The T(sci)C stem is stacked onto the acceptor stem appearing like one helix (T/F) - correct answer
✔✔True



The anticodon stem is stacked on the D-loop appearing like one helix (T/F) - correct answer ✔✔False
(Stacked on the D-stem)



The D-loop always contained at least one dihydrouridine (T/F) - correct answer ✔✔True



For most genes the sigma 70 determines where RNA polymeras woll began transcription by binding the -
10/-35 sequences (T/F) - correct answer ✔✔True



RNA polymerase posses 3'-5' exonuclease prrofreading activity (T/F) - correct answer ✔✔False (does not
do this)



The addition of each nucleotide residue to the RNA chain results from attack by the 5'-OH of the mRNA
on the α-phosphate of the incoming ribonucleoside triphosphate (NTP) (T/F) - correct answer ✔✔False
(3'-OH)



RNA polymerase activity resides on the α subunit of RNA polymerase. (T/F) - correct answer ✔✔False
(beta Subunit)



The RNA polymerase core complex can interact with a new σ subunit, even one of a different type, each
time it transcribes a new gene (T/F) - correct answer ✔✔True