SJSU BIOL 30 LAB EXAM 1 questions
and answers
Which food product will we be isolating genomic DNA from today?✔✔corn/maize
What is the name of the gene that encodes the Bt endotoxin?✔✔Cry1A
What does PCR stand for?✔✔Polymerase Chain Reaction
All of the following are part of a PCR reaction except:✔✔Bt Toxin
What is the name of the gene that will be PCR'd that is specific to maize?✔✔irvl
What is the charge of DNA?✔✔DNA has a negative charge.
What will you stain the DNA with after you run the gel?✔✔After running the gel, the
DNA is stained with Ethidium Bromide.
What is the color of the dye that helps you se that the DNA is moving into the
gel?✔✔The loading dye that helps you see the moving DNA is red.
Approximately what voltage will you use to move the DNA into the gel?✔✔The
voltage will be increased to 120 V in order to move the DNA into the gel.
What is the percentage of the agarose that you will be using in lab today?✔✔The gel
is 2.5% agarose.
How many primers are added to our test tube? What are they?✔✔2 pairs of primers;
cry1A forward, cry1A reverse, ivr1 forward, ivr1 reverse
If you placed one copy of your gene into a tube and rand a PCR for 3 cycles, how
many copies of your gene would you have at the end?✔✔8 copies of the gene (2^n
= 2^3 = 8)
How will we be able to tell if our corn product is GMO or not? Make sure to discuss
what you are measuring that allows you to know this.✔✔If the product is not GMO,
PCR will only produce 226 bp ivr1 product. If it is GMO, PCR will produce both 226
bp ivr1 and 133bp cry1A. You are measuring the base pair sequences to identify if
the product was genetically modified with cry1A.
Draw the gel you would expect to get if you loaded lane 1 with your DNA ladder, lane
2 with your negative control, lane 3 with a GMO corn product, and lane 4 with a non-
GMO corn product.✔✔Lane 1 -- DNA ladder
Lane 2 -- primer dimer
Lane 3 -- 226 bp (ivr1), 133 bp (cry1A), primer dimer
Lane 4 -- 226 bp (ivr1), primer dimer
and answers
Which food product will we be isolating genomic DNA from today?✔✔corn/maize
What is the name of the gene that encodes the Bt endotoxin?✔✔Cry1A
What does PCR stand for?✔✔Polymerase Chain Reaction
All of the following are part of a PCR reaction except:✔✔Bt Toxin
What is the name of the gene that will be PCR'd that is specific to maize?✔✔irvl
What is the charge of DNA?✔✔DNA has a negative charge.
What will you stain the DNA with after you run the gel?✔✔After running the gel, the
DNA is stained with Ethidium Bromide.
What is the color of the dye that helps you se that the DNA is moving into the
gel?✔✔The loading dye that helps you see the moving DNA is red.
Approximately what voltage will you use to move the DNA into the gel?✔✔The
voltage will be increased to 120 V in order to move the DNA into the gel.
What is the percentage of the agarose that you will be using in lab today?✔✔The gel
is 2.5% agarose.
How many primers are added to our test tube? What are they?✔✔2 pairs of primers;
cry1A forward, cry1A reverse, ivr1 forward, ivr1 reverse
If you placed one copy of your gene into a tube and rand a PCR for 3 cycles, how
many copies of your gene would you have at the end?✔✔8 copies of the gene (2^n
= 2^3 = 8)
How will we be able to tell if our corn product is GMO or not? Make sure to discuss
what you are measuring that allows you to know this.✔✔If the product is not GMO,
PCR will only produce 226 bp ivr1 product. If it is GMO, PCR will produce both 226
bp ivr1 and 133bp cry1A. You are measuring the base pair sequences to identify if
the product was genetically modified with cry1A.
Draw the gel you would expect to get if you loaded lane 1 with your DNA ladder, lane
2 with your negative control, lane 3 with a GMO corn product, and lane 4 with a non-
GMO corn product.✔✔Lane 1 -- DNA ladder
Lane 2 -- primer dimer
Lane 3 -- 226 bp (ivr1), 133 bp (cry1A), primer dimer
Lane 4 -- 226 bp (ivr1), primer dimer
