HISTOTECHNOLOGY I FINAL EXAM PREP 2024 WITH 100% ACCURATE ANSWERS

HISTOTECHNOLOGY I FINAL EXAM
PREP 2024 WITH 100% ACCURATE
ANSWERS

,HISTOTECHNOLOGY I FINAL EXAM
PREP 2024 WITH 100% ACCURATE
ANSWERS
Tape coverslips - CORRECT ANSWERSused with robotic coverslip instruments; acetate film coated with
adhesive on one side, activated by xylene



Combined coverslip and mountant - CORRECT ANSWERSapplied to slide by pouring, dipping or spraying;
varnish-like substance that covers and protects section (does not require a glass coverslip)



Synthetic Resinous Mounting Medium - CORRECT ANSWERS1. dries quickly and when dried it isnt sticky

2. neutral pH

3. used with solvents (should match the clearing agent)

4. more volatile solvents produce more latent air bubbles

5. RI 1.5-1.55



What are the two common staining methods for nucleic acid in histology? - CORRECT ANSWERS1.
Feulgen Reaction

2. Methyl Green-Pyronin Method (DNA/RNA)



Feulgen Reaction tests for what? - CORRECT ANSWERSDNA



What does the Schiff reagent bind to in the Feulgen Reaction? - CORRECT ANSWERSAldehyde group
created by the hydrolysis of DNA



Why can't we use the Bouin fixative on tissues being tested with Feulgen? - CORRECT ANSWERSBouin
hydrolyzes tissues



In Feulgen reactions, what happens when the hydrolysis reactions are uncontrolled? - CORRECT
ANSWERSnegative results occur

, Methyl Green-Pyronin Y is used to demonstrate what? - CORRECT ANSWERSdifferentiates between DNA
and RNA, and identify plasma cells or immunoblasts



Where are high concentrations of immunoblasts and plasma cells found within the cell? - CORRECT
ANSWERSRibosomes



What does the Methyl Green-Pyronin Y bind selectively to? - CORRECT ANSWERSPolymerization in
DNA/RNA



What should you use as a control slide in the Methyl Green-Pyronin Y reaction? - CORRECT ANSWERSA
positive slide for plasma cells



Polychromatic stains result in what color results? - CORRECT ANSWERSMultiple colored tissue elements



Polychromatic dyes are - CORRECT ANSWERSsingle stain solutions that contain multiple dye molecules



What are two examples of polychromatic stains? - CORRECT ANSWERS1. Gomori 1-step Trichrome

2. Romanowsky stains



Can polychromatic stains stain orthochromatically, metachromatically or both? - CORRECT
ANSWERSBOTH



Romanowsky stains are a combination of what and what? - CORRECT ANSWERSEosin

Methylene Blue



Eosin is an acid or basic dye? - CORRECT ANSWERSAcid dye



Methylene Blue is an acid or basic dye? - CORRECT ANSWERSBasic dye