Campbell Biology Ch. 6 - 7 Test Bank
1.1) When biologists wish to study the internal ultrastructure of cells, they
can achieve the finest resolution by using
A) a phase-contrast light microscope.
B) a scanning electron microscope.
C) a transmission electronic microscope.
D) a confocal fluorescence microscope.
E) a super-resolution fluorescence microscope.: C
2.2) The advantage of light microscopy over electron microscopy is that
A) light microscopy provides for higher magnification than electron
mi- croscopy.
B) light microscopy provides for higher resolving power than electron
mi- croscopy.
C) light microscopy allows one to view dynamic processes in living cells.
D) light microscopy provides higher contrast than electron microscopy.
E) specimen preparation for light microcopy does not produce artifacts.: c
3.3) A primary objective of cell fractionation is to
A) view the structure of cell membranes.
B) sort cells based on their size and weight.
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C) determine the size of various organelles.
D) separate the major organelles so that their particular functions can be
determined.
E) separate lipid-soluble from water-soluble molecules.: Answer: D
4.4) In the fractionation of homogenized cells using centrifugation, the
prima- ry factor that determines whether a specific cellular component ends
up in the supernatant or the pellet is
A) the relative solubility of the component.
B) the size and weight of the component.
C) the percentage of carbohydrates in the component.
D) the presence or absence of nucleic acids in the component.
E) the presence or absence of lipids in the component.: B
5.5) Which of the following correctly lists the order in which cellular com-
ponents will be found in the pellet when homogenized cells are treated
with increasingly rapid spins in a centrifuge?
A) ribosomes, nucleus, mitochondria
B) chloroplasts, ribosomes, vacuoles
C) nucleus, ribosomes, chloroplasts
D) vacuoles, ribosomes, nucleus
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E) nucleus, mitochondria, ribosomes: E
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6.6) Green fluorescent protein (GFP) can be used to fluorescently label a
specific protein in cells by genetically engineering cells to synthesize the
target protein fused to GFP. What is the advantage of using GFP fusions to
visualize specific proteins, instead of staining cells with fluorescently
labeled probes that bind to the target protein?
A) GFP fusions enable one to track changes in the location of the protein
in living cells; staining usually requires preserved cells.
B) GFP fusions enable higher resolution than staining with
fluorescent probes.
C) GFP permits the position of the protein in the cell more precisely
than fluorescent probes.
D) GFP permits visualization of protein-protein interactions;
fluorescent probes do not.
E) GFP fusions are not subject to artifacts; fluorescent probes may
introduce background artifacts.: A
7.7) What is the reason that a modern electron microscope (TEM) can
resolve biological images to the subnanometer level, as opposed to tens of
nanome- ters achievable for the best super-resolution light microscope?
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1.1) When biologists wish to study the internal ultrastructure of cells, they
can achieve the finest resolution by using
A) a phase-contrast light microscope.
B) a scanning electron microscope.
C) a transmission electronic microscope.
D) a confocal fluorescence microscope.
E) a super-resolution fluorescence microscope.: C
2.2) The advantage of light microscopy over electron microscopy is that
A) light microscopy provides for higher magnification than electron
mi- croscopy.
B) light microscopy provides for higher resolving power than electron
mi- croscopy.
C) light microscopy allows one to view dynamic processes in living cells.
D) light microscopy provides higher contrast than electron microscopy.
E) specimen preparation for light microcopy does not produce artifacts.: c
3.3) A primary objective of cell fractionation is to
A) view the structure of cell membranes.
B) sort cells based on their size and weight.
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102
, Campbell Biology Ch. 6 - 7 Test Bank
C) determine the size of various organelles.
D) separate the major organelles so that their particular functions can be
determined.
E) separate lipid-soluble from water-soluble molecules.: Answer: D
4.4) In the fractionation of homogenized cells using centrifugation, the
prima- ry factor that determines whether a specific cellular component ends
up in the supernatant or the pellet is
A) the relative solubility of the component.
B) the size and weight of the component.
C) the percentage of carbohydrates in the component.
D) the presence or absence of nucleic acids in the component.
E) the presence or absence of lipids in the component.: B
5.5) Which of the following correctly lists the order in which cellular com-
ponents will be found in the pellet when homogenized cells are treated
with increasingly rapid spins in a centrifuge?
A) ribosomes, nucleus, mitochondria
B) chloroplasts, ribosomes, vacuoles
C) nucleus, ribosomes, chloroplasts
D) vacuoles, ribosomes, nucleus
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E) nucleus, mitochondria, ribosomes: E
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6.6) Green fluorescent protein (GFP) can be used to fluorescently label a
specific protein in cells by genetically engineering cells to synthesize the
target protein fused to GFP. What is the advantage of using GFP fusions to
visualize specific proteins, instead of staining cells with fluorescently
labeled probes that bind to the target protein?
A) GFP fusions enable one to track changes in the location of the protein
in living cells; staining usually requires preserved cells.
B) GFP fusions enable higher resolution than staining with
fluorescent probes.
C) GFP permits the position of the protein in the cell more precisely
than fluorescent probes.
D) GFP permits visualization of protein-protein interactions;
fluorescent probes do not.
E) GFP fusions are not subject to artifacts; fluorescent probes may
introduce background artifacts.: A
7.7) What is the reason that a modern electron microscope (TEM) can
resolve biological images to the subnanometer level, as opposed to tens of
nanome- ters achievable for the best super-resolution light microscope?
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