ASCP MLT Chemistry Practice Questions and Answers

ASCP MLT Chemistry Practice Questions and Answers Which element in proteins differentiates substance from carbohydrates and lipids? - Answer️️ -nitrogen What proteins is soluble in water? - Answer️️ -albumin How are amino acids joined together to form a chain like structure? - Answer️️ - peptide bonding Proteins may become denatured when subjected to? - Answer️️ -mechanical agitation, heat, chemical treatments What does denaturation of proteins refer to? - Answer️️ -alteration in tertiary structure Which aminoacidopathy is NOT caused by an enzyme deficiency? - Answer️️ - cystinuria How many immunoglobulin classes are currently recognized? - Answer️️ -five which reagent is employed in the serum protein determination? - Answer️️ -biuret What should hemolyzed serum not be used for total protein analysis? - Answer️️ - hemoglobin is absorbed at the same wavelength as protein in the reagent What is NOT true of the biuret reaction? - Answer️️ -is relatively free from interference by lipids and hemoglobin ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM Page | 2 Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - Answer️️ -there is less interference from pigment (s) What is the most anodic protein on electrophoresis at pH 8.6? - Answer️️ -albumin A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen in which disease state? - Answer️️ -acute inflammation If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10% beta- globulin, and 45% gamma-globulin, then what condition may exist in the patient? - Answer️️ -monoclonal gammopathy Glycoproteins and mucoproteins are usually bound to which substance? - Answer️️ - carbohydrates Name some glycoproteins? - Answer️️ -ceruloplasmin, follicle-stimulating hormone, cryoglobulin What applies to cryoglobulins? - Answer️️ -they are temperature-sensitive proteins What is true of albumen? - Answer️️ -it is produced in the liver and it is water soluble Decreased alpha-fetoprotein values are associated with what condition? - Answer️️ - Down Syndrome What protein transports iron? - Answer️️ -transferrin In acute renal failure, what non protein nitrogen rises the fastest? - Answer️️ -blood urea nitrogen By the urease method, urea is enzymatically converted to which end product? - Answer️️ -ammonia ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM Page | 3 Why can untreated urine be used for the determination of urea by the diacetyl monoxime method? - Answer️️ -The method is not measureing ammonia An elevated creatinine value is most likely to be accompanied by what values? - Answer️️ -elevated blood urea nitrogen, 10X creatinine value What is the classical method for creatinine reaction? - Answer️️ -Jaffe The measurement of creatinine is based on the formation of a yellow-red color and which reagent? - Answer️️ -titan yellow The creatinine clearance test is based on what assumption? - Answer️️ -Creatinine passes into the ultrafiltrate What is the most common clearance test used to measure the glomerular filtration rate? - Answer️️ -creatinine Creatinine clearance formula - Answer️️ -UV/P or UV/P (1.73/correction factor) 1440 mls/min A creatinine clearance test is performed. The 24-hour ur