Exam (elaborations) BIOCHEM CHAPTER 20

BIOCHEM FINAL CHAPTER 20 EXAM
20
Oxidative phosphorylation - CORRECT ANSWER-transfer of a phosphate group linked
to electron transfer reaction in which where oxygen is the final acceptor of the electron

substrate level phosphorylation - CORRECT ANSWER-The enzyme-catalyzed
formation of ATP by direct transfer of a phosphate group to ADP from an intermediate
substrate in catabolism. (glycolysis (steps 7 and 10) and TCA (steps 5))

Where does glycolysis occur - CORRECT ANSWER-cytoplasm

Where does the citric acid cycle occur? - CORRECT ANSWER-mitochondrial matrix

Is NADH or FADH2 more effective? - CORRECT ANSWER-NADH (2.5 vs 1.5 moles of
ATP)

Negative Reduction potential - CORRECT ANSWER-a compound for which reduction is
less favorable

Positive Reduction potential - CORRECT ANSWER-a compound for which oxidation is
less favorable

Complex One ETC - CORRECT ANSWER-Location: Imbedded in the intermembrane
Enzyme: NADH-CoQ oxidoreductase
Overall Reaction: NADH + (H+) + CoQ --> (NAD+) + CoQH2
Able to phosphorylate ATP: yes

Complex Two ETC - CORRECT ANSWER-Location: Located in the mitochondrial
matrix
Enzyme: Succinate-coenzyme Q oxidoreductase
Overall Reaction: Succinate + CoQ -> Fumarate + CoQH2
Able to phosphorylate ATP: no

Complex Three ETC - CORRECT ANSWER-Location: Imbedded in the intermembrane
Enzyme: CoQH2-cytochrome c oxidoreductase
Overall Reaction: CoQH2 + 2Cyt c[Fe(III)] --> CoQ + 2Cyt c[Fe(II)] + 2H+
Able to phosphorylate ATP: yes

Complex Four ETC - CORRECT ANSWER-Location: Imbedded in the intermembrane