A CRP test can screen for correct answers - infection
- chronic inflammatory disease
- heart disease
C-Reactive protein correct answers a substance in the serum of patients with acute
inflammation that reacted with the C polysaccharide of pneumococcus
- alpha-globulin produced in the liver
- not specific
- CRP combines to T-lymphocyte to inhibit their functions
CRP Testing correct answers used for in vitro diagnostic
- quantitative test
- testing keeps track of infections and diseases that cause inflammation
CRP Reagent correct answers suspension of polystyrene latex particles coated with
IgG fraction of an anti-human CRP serum
Agglutination occurs when... correct answers the CRP in the serum reacts with the IgG
coated to the latex particles
If positive for the CRP... correct answers confirm using a titer
- however, confirmatory not necessary
Reagent Storage correct answers stored between 2-8C
- do not freeze
Procedure correct answers - allow reagents and samples to warm to room temperature
- suspend latex in buffer solution
- place 50uL of serum onto the test card
- place one drop of reagent next to drop of the serum
- mix with stirrer
- put on rotary for 2 minutes
- check for agglutination
Semiquantitative Test correct answers - place 50uL of saline on sections 2-6
- place 50uL of serum on sections 1 and 2
- mix and transfer 50uL and discard of last
- add one drop of reagent to each section
- rotary for 2 minutes
(1:32 dilution)