BIOCHEMISTRY WORKSHOP 2024/2025
WITH 32 QUESTIONS AND CORRECT
ANSWERS RATED A+
what is the mechanism of citrate - ANSWER-calcium chelation
what is the most commonly used anticoagulant for coagulation testing - ANSWER-
citrate
it 3 carboxyl group will readily bind (chelate) calcium preventing calcium from
reacting with substrate in the coagulation cascade
EDTA calcium chelator is primary used for - ANSWER-CBC because it is too strong
what is the laboratory anticoagulant of choice for blood collection that requires
analysis of ionized calcium , pH, or blood gasses - ANSWER-heparin which activate
antithrombin III to neutralize factor IX, X and II
platelet rich plasma PRP is used for - ANSWER-platelet aggregometry
it lacks RBC and wBC but still contain platelets
, primary hemostasis test, tests for - ANSWER-CBC platelet counts
bleeding time
platelet aggregometry
Bleeding time evaluates - ANSWER-platelet function and vascular constriction by
measuring the time required to stop the flow of blood from a standard wound.
what does platelet aggregometry involves - ANSWER-the measurement of light
transmission through a sample of PRP
why is the bleeding time test doesn't measure the ability of coagulation factors to
forms a fibrin clot which keeps the platelets plug in place - ANSWER-because the
patient might have rebleeding when they more later if their coagulation system is
impaired but the bleeding time test will be finished by then.
what test is considered the gold standard test for platelet function - ANSWER-
platelet aggregometry
PRP with resting platelets -> light mostly blocked
the addition of an agonist (platelet activator) to PRP and subsequent aggregation
of platelet result in increased transmission of light due to clumping
WITH 32 QUESTIONS AND CORRECT
ANSWERS RATED A+
what is the mechanism of citrate - ANSWER-calcium chelation
what is the most commonly used anticoagulant for coagulation testing - ANSWER-
citrate
it 3 carboxyl group will readily bind (chelate) calcium preventing calcium from
reacting with substrate in the coagulation cascade
EDTA calcium chelator is primary used for - ANSWER-CBC because it is too strong
what is the laboratory anticoagulant of choice for blood collection that requires
analysis of ionized calcium , pH, or blood gasses - ANSWER-heparin which activate
antithrombin III to neutralize factor IX, X and II
platelet rich plasma PRP is used for - ANSWER-platelet aggregometry
it lacks RBC and wBC but still contain platelets
, primary hemostasis test, tests for - ANSWER-CBC platelet counts
bleeding time
platelet aggregometry
Bleeding time evaluates - ANSWER-platelet function and vascular constriction by
measuring the time required to stop the flow of blood from a standard wound.
what does platelet aggregometry involves - ANSWER-the measurement of light
transmission through a sample of PRP
why is the bleeding time test doesn't measure the ability of coagulation factors to
forms a fibrin clot which keeps the platelets plug in place - ANSWER-because the
patient might have rebleeding when they more later if their coagulation system is
impaired but the bleeding time test will be finished by then.
what test is considered the gold standard test for platelet function - ANSWER-
platelet aggregometry
PRP with resting platelets -> light mostly blocked
the addition of an agonist (platelet activator) to PRP and subsequent aggregation
of platelet result in increased transmission of light due to clumping
