BIO 311C Sata Final Exam Review Questions And Answers With Verified Solutions Graded A+

4. Which of the following sequence is the correct one for replicating DNA? Not all the steps are included. I. RNA Primers anneal to the DNA template II. ssDNA binding proteins bind to stabilize single strand DNA after helicase unwinds it. III. RNA primers are removed and new DNA strand is made by DNA polymerase IIV. DNA ligase connects the DNA fragments V. Complementary copy of DNA is made by DNA polymerase III A) I, II, III, IV, V B) II, III, IV, I, V C) V, I, III, II, IV D) II, I, V, III, IV E) II, V, I, III, IV, V - D A culture of E. coli bacteria were grown in media containing 15N isotope resulting in both strands of DNA with the heavier 15N. What would be the composition of DNA if those bacteria were transferred and allowed to replicate 20 times in the presence of a lighter 14N isotope? A) Neither of the two DNA strands would contain 14N as they will continue to be heavy with 15N. B) DNA in both strands will continue to be of medium density with 15N and 14N. C) One fourth of the DNA will be 15N-15N and the rest 15N-14N. D) The proportion of DNA with 15N -14N will be much lower than the DNA with 14N-14N. E) One of the daughter cells, but not the other, would have 15N-15N. All others will have 15N-14N. - D A DNA template of 5' to 3 'direction is copied from the other direction starting at the 3' end of the template DNA. This is because A) the 5' to 3' direction of one strand runs counter to the 5' to 3' direction of the other strand.B) DNA polymerase adds nucleotides only at the 3 'end of the new strand. C) base pairings create unequal spacing between the two DNA strands. D) The helical nature of DNA makes the DNA strands copied in opposite direction. E) Both A and B are important reasons for this process. - E A scientist wants to study if the gene encoding RubisCO, a key enzyme in photosynthesiswill be transcribed upon exposure of light. What will be a good technique to study the gene expression? A) Southern B) Northern C) microarray D) PCR E) Western - B A technique that can be used to study the expression of 10,000 genes in one experiment is ________ A) Southern B) Northern C) microarray D) PCR E) Western - C