BIOMG 2801 Final QUESTION AND CORRECT ANSWER 100%

BIOMG 2801 Final QUESTION AND CORRECT ANSWER 100% What media would WT grow on - CORRECT ANSWER-LB, min, min + ala, min + lac, What media would ala- grow on - CORRECT ANSWER-LB, min + ala What media would lac- grow on - CORRECT ANSWER-LB, min, min + ala EMS causes - CORRECT ANSWER-point mutations Transitions - CORRECT ANSWER-A--G, C--T Transversions - CORRECT ANSWER-A--C, G--T Tn10 causes - CORRECT ANSWER-insertions, deletions Why was CSH61 Hfr grown on min first - CORRECT ANSWER-to minimize the amount of circular chromosomes to increase mutagenization success Why was CSH61 centrifuged - CORRECT ANSWER-to add O2 to increase bacterial growth Stages of bacterial growth - CORRECT ANSWER-Lag, log, stationary Lag phase - CORRECT ANSWER-slow growth bacteria is waiting for a more favorable environment Log phase - CORRECT ANSWER-quickest growth binary fission Stationary phase - CORRECT ANSWER-Waste build up nutrient exhaustion Why grow for 120 mins - CORRECT ANSWER-want to observe bacteria at log phase phenotypic lag - CORRECT ANSWER-functional enzymes are still initially present segregation lag - CORRECT ANSWER-takes several segregation events to isolate mutated chromosomes Indicator media - CORRECT ANSWER-MacConkey's Selective media - CORRECT ANSWER-min for auxotrophs 0 pool plates - CORRECT ANSWER-auxotrophic for 2, supplements are not provided 1 pool plate - CORRECT ANSWER-need 2 or 3 amino acids that are on the same plate, double mutant 3 pool plates - CORRECT ANSWER-isoleucine, scavenging for chemically or structurally similar AA 4 pool plates - CORRECT ANSWER-mixed culture, 2 colonies Controls for experiment 2 - CORRECT ANSWER-1. against naturally occurring mutations, non-mutagenized cells 2. dilutions 3. picked and grided on min first 4. min plate control to confirm auxotrophs 5. prototroph streak on master plate to ensure plates can support growth 6. LB plate last for transfer efficiency ex conj chromosome - CORRECT ANSWER-lac-, pro+, StptRNA, AbR Hfr lac- chromosome - CORRECT ANSWER-lac-, pro+, WT tRNA, AbS F- chromosome - CORRECT ANSWER-lac-pro- deletion, StptRNA, AbR CSH54 (F) genotype - CORRECT ANSWER-pro- strepR CSH55 (E) genotype - CORRECT ANSWER-pro- nalR CSH56 (D) genotype - CORRECT ANSWER-pro- nalR lac- genotype - CORRECT ANSWER-pro+ strepS nalS Su3 - CORRECT ANSWER-F // tyrosine Su2 - CORRECT ANSWER-E // glutamine Su1 - CORRECT ANSWER-D // serine Controls for experiment 3 - CORRECT ANSWER-1. min + ab plate - Hfr cells are sensitive to antibiotics - F- cells are pro2. MacConkey's plate: how lac+ vs lac- looks like