BACE EXAM QUESTIONS AND
ANSWERS
4.651 x 10^4 written in expanded form is - ANSWER 46,510
What is 5.78 x 3.13, rounded to the correct number of significant
digits? - ANSWER 18.1
What is 4.202 x 0.806, rounded to the correct number of significant
digits? - ANSWER 3.39
How many grams is 2.6 kg? - ANSWER 2600
How many milliliters is 0.725L?
Group of answer choices - ANSWER 725
Your supervisor provides you with a 1.0 mg/mL solution of Bovine
Serum Albumin (BSA), and asks you to make a 5-step 2-fold serial
dilution. What would be the dilution factor at step 3? - ANSWER 1:8
You are given a 5.0 mg/mL protein standard solution, and asked to
prepare a 5-step, 2-fold serial dilution to create a standard curve.
What is the final concentration of protein standard in mg/mL? Record
the correct amount to three decimal places. - ANSWER 0.156
You are given a 5.0 mg/mL protein standard solution, and asked to
prepare a 5-step, 2-fold serial dilution to create a standard curve.
After the third step of the dilution, what is the concentration of the
protein standard, in mg/mL? Record the correct amount to two
decimal places. - ANSWER 0.63
Your supervisor provides you with a 1.0 mg/mL solution of Bovine
Gamma Globulin (BGG). What is the dilution factor if you add 150 µL
of 1.0 mg/mL BGG to 150 µL of diluent? - ANSWER 1:2
A technician is asked to prepare a dilution of a common laboratory
,disinfectant. The label indicates that a 1:29 dilution in water is
required prior to use. Using the simple dilution method, calculate the
volume in mL of disinfectant required to make 1.0 L. Record the
answer to one decimal place below - ANSWER 34.5
An SOP for preparing a bleach solution instructs to dilute the bleach
1:15 in water. Using the simple dilution method, calculate the volume
in mL of bleach required to make 3.5 L. Record the correct amount to
one decimal place. - ANSWER 233.3 mL
You are given a stock protein solution with a concentration of 24
mg/mL. Determine the protein concentration of a solution made by
mixing 5 μl of the stock with 45 μl of a buffer. Record the amount to
one decimal place. - ANSWER 2.4
Calculate the volume in mL of 50X TE Buffer required to make 1.5 L of
1X TE Buffer. - ANSWER 30 mL
Calculate the volume in mL of 25X TAE Buffer required to make 5.0 L
of 1X TAE Buffer. Record the correct amount. - ANSWER 200 mL
Calculate the volume in mL of a 10.5 mg/mL tetracycline stock
solution required to make 525 mL LB with a final concentration of
tetracycline at 2.75 mg/mL. Record the answer to one decimal place.
- ANSWER 137.5
Which of the following is the correct method to make 1 L of 10%
sodium chloride? - ANSWER Dissolve the appropriate amount of
sodium chloride in a small volume of dH2O, then adjust the volume to
1L
Calculate the mass in g of agarose required to make 200 mL of a
1.2% agarose gel. Record the answer to one decimal place. -
ANSWER 2.4
Calculate the mass in mg of kanamycin required to make 25 mL of a
12 mg/mL solution of kanamycin. - ANSWER 300
Calculate the mass in g of sucrose required to make 125 mL of a
, 15.5% (w/v) sucrose solution. Record the correct amount to one
decimal place. - ANSWER 19.4
Calculate the mass in g of MgCl2 required to make 75 mL of a 20%
(w/v) MgCl2 solution. Record the correct amount. - ANSWER 15
Calculate the volume in mL of a 10.0 M solution of HCl stock solution
required to make 250 mL of 1.0 M HCl? Record the correct amount. -
ANSWER 25
Calculate the volume in mL of a 1.5 M solution of NaOH stock solution
required to make 275 mL of 50.0 mM NaOH? Record the answer to
one decimal place. - ANSWER 9.2
Calculate the molarity of a solution containing 0.42 moles of NaCl in
2.5 L of water? Record the answer to two decimal places. -
ANSWER .17
To dilute a bacterial culture, 10 uL of culture is mixed with fresh
culture medium to a final volume of 0.5 mL. The culture was diluted
by a factor of: - ANSWER 50
bleach - ANSWER Basic
lemon juice - ANSWER Acidic
Baking soda - ANSWER Basic
Oven cleaner - ANSWER Basic
vinegar - ANSWER Acidic
NaCl bond type - ANSWER ionic bond
H20 bond type - ANSWER Polar covalent
ANSWERS
4.651 x 10^4 written in expanded form is - ANSWER 46,510
What is 5.78 x 3.13, rounded to the correct number of significant
digits? - ANSWER 18.1
What is 4.202 x 0.806, rounded to the correct number of significant
digits? - ANSWER 3.39
How many grams is 2.6 kg? - ANSWER 2600
How many milliliters is 0.725L?
Group of answer choices - ANSWER 725
Your supervisor provides you with a 1.0 mg/mL solution of Bovine
Serum Albumin (BSA), and asks you to make a 5-step 2-fold serial
dilution. What would be the dilution factor at step 3? - ANSWER 1:8
You are given a 5.0 mg/mL protein standard solution, and asked to
prepare a 5-step, 2-fold serial dilution to create a standard curve.
What is the final concentration of protein standard in mg/mL? Record
the correct amount to three decimal places. - ANSWER 0.156
You are given a 5.0 mg/mL protein standard solution, and asked to
prepare a 5-step, 2-fold serial dilution to create a standard curve.
After the third step of the dilution, what is the concentration of the
protein standard, in mg/mL? Record the correct amount to two
decimal places. - ANSWER 0.63
Your supervisor provides you with a 1.0 mg/mL solution of Bovine
Gamma Globulin (BGG). What is the dilution factor if you add 150 µL
of 1.0 mg/mL BGG to 150 µL of diluent? - ANSWER 1:2
A technician is asked to prepare a dilution of a common laboratory
,disinfectant. The label indicates that a 1:29 dilution in water is
required prior to use. Using the simple dilution method, calculate the
volume in mL of disinfectant required to make 1.0 L. Record the
answer to one decimal place below - ANSWER 34.5
An SOP for preparing a bleach solution instructs to dilute the bleach
1:15 in water. Using the simple dilution method, calculate the volume
in mL of bleach required to make 3.5 L. Record the correct amount to
one decimal place. - ANSWER 233.3 mL
You are given a stock protein solution with a concentration of 24
mg/mL. Determine the protein concentration of a solution made by
mixing 5 μl of the stock with 45 μl of a buffer. Record the amount to
one decimal place. - ANSWER 2.4
Calculate the volume in mL of 50X TE Buffer required to make 1.5 L of
1X TE Buffer. - ANSWER 30 mL
Calculate the volume in mL of 25X TAE Buffer required to make 5.0 L
of 1X TAE Buffer. Record the correct amount. - ANSWER 200 mL
Calculate the volume in mL of a 10.5 mg/mL tetracycline stock
solution required to make 525 mL LB with a final concentration of
tetracycline at 2.75 mg/mL. Record the answer to one decimal place.
- ANSWER 137.5
Which of the following is the correct method to make 1 L of 10%
sodium chloride? - ANSWER Dissolve the appropriate amount of
sodium chloride in a small volume of dH2O, then adjust the volume to
1L
Calculate the mass in g of agarose required to make 200 mL of a
1.2% agarose gel. Record the answer to one decimal place. -
ANSWER 2.4
Calculate the mass in mg of kanamycin required to make 25 mL of a
12 mg/mL solution of kanamycin. - ANSWER 300
Calculate the mass in g of sucrose required to make 125 mL of a
, 15.5% (w/v) sucrose solution. Record the correct amount to one
decimal place. - ANSWER 19.4
Calculate the mass in g of MgCl2 required to make 75 mL of a 20%
(w/v) MgCl2 solution. Record the correct amount. - ANSWER 15
Calculate the volume in mL of a 10.0 M solution of HCl stock solution
required to make 250 mL of 1.0 M HCl? Record the correct amount. -
ANSWER 25
Calculate the volume in mL of a 1.5 M solution of NaOH stock solution
required to make 275 mL of 50.0 mM NaOH? Record the answer to
one decimal place. - ANSWER 9.2
Calculate the molarity of a solution containing 0.42 moles of NaCl in
2.5 L of water? Record the answer to two decimal places. -
ANSWER .17
To dilute a bacterial culture, 10 uL of culture is mixed with fresh
culture medium to a final volume of 0.5 mL. The culture was diluted
by a factor of: - ANSWER 50
bleach - ANSWER Basic
lemon juice - ANSWER Acidic
Baking soda - ANSWER Basic
Oven cleaner - ANSWER Basic
vinegar - ANSWER Acidic
NaCl bond type - ANSWER ionic bond
H20 bond type - ANSWER Polar covalent
