HPLC Chromatograms
I will be analysing different solvents used to extract a sample of physica semipinnata (rosette lichen)
against a reference.
Explain how to calculate peak area. Include an example calculation.
To calculate the peak are you must measure the heigh of the peak and multiply it by the half of the
width. it cam be represented as;
Height of peak/ hath of the width of peak
Example
Height is 15
Width is 4
The it will be
7 /2 = 3.5
3.5 is the peak area
, Reference Chromatogram.
Reference compounds
Peak number Standards for Compounds present in physcia
semipinnata
1 lecanoric acid
2 methyl-β-orcinol carboxylate
3 ethyl haematommate
4 evernic acid
5 obtusic acid
6 atranorin
I will be analysing different solvents used to extract a sample of physica semipinnata (rosette lichen)
against a reference.
Explain how to calculate peak area. Include an example calculation.
To calculate the peak are you must measure the heigh of the peak and multiply it by the half of the
width. it cam be represented as;
Height of peak/ hath of the width of peak
Example
Height is 15
Width is 4
The it will be
7 /2 = 3.5
3.5 is the peak area
, Reference Chromatogram.
Reference compounds
Peak number Standards for Compounds present in physcia
semipinnata
1 lecanoric acid
2 methyl-β-orcinol carboxylate
3 ethyl haematommate
4 evernic acid
5 obtusic acid
6 atranorin
