Chromatography (TLC): Lab with perfect answers 2024

Why shouldn't the start line, where the solution mixtures are initially placed, be drawn in ink? correct answersThe ink is likely to dissolve in the solvent and will add extra spots to the chromatogram. What is the Rf value? correct answersThe retention factor (Rf) for each component is the ratio of the distance traveled by the component to the distance traveled by the solvent front. When setting up a column, what is the reason for adding a layer of sand on top of the absorbent column? correct answersTo prevent disruption of the surface when solvent is added SAFETY: TLC spotters are sharp. The MAJOR safety concern when using spotters is that: correct answersThey can cause stab injuries SAFETY: Apart from staining your skin, the stains used to expose the spots on a TLC plate pose no safety hazard. correct answersFalse SAFETY: Which of these are safety concerns related to the use of UV light to expose spots on a TLC plate? correct answersIt can damage your eyes if looked at directly Why should the solvent level in the elation tank be below the level of the baseline of the TLC plate? correct answersThe compound in the spots would dissolve into the solvent if it were higher How long should you run a TLC plate for? correct answersUntil the solvent front is just below the top of the plate SAFETY: Why is it important to handle silica powder in fume cupboard? More than one answer may be correct. correct answers▪︎The powder, if inhaled, can cause problems with the respiratory tract. ▪︎The powder is fine and easily inhaled SAFETY: Which of the following statements about the procedure for column chromatography are true? More than one answer may be correct. correct answers▪︎Check for cracks in the glass before pressurising the system ▪︎Apply enough pressure to increase the flow rate to a reasonable degree SAFETY: How should the silica be disposed of at the end of the experiment? correct answersIn the appropriate waste solid container