Final- 3. Direct Antiglobulin Test (DAT 2024/2025) 100% Correct Answers

Final- 3. Direct Antiglobulin Test (DAT 2024/2025) 100% Correct Answers Principle of the Anti-Human Globulin Test ____ antibodies readily cause agglutination. ____ antibodies do not cause agglutination. In order to detect the presence of ____ antibodies, we must add an antibody to the Fc portion of the antibody we wish to detect. the antibody that we add goes by many names: anti-human globulin, AHG, Coombs antibody, Coomb's serum, or the green stuff. After Centrifugation, coated red cells (or ___ RBCs) form an antibody-antigen bridge via the ____ antibody resulting in visible agglutination. - CORRECT ANSWER-IgM antibodies cause agglutination. IgG do not IgG antibodies coated red cells = sensitized RBCs AHG antibody When do we use AHG technique? - CORRECT ANSWER-In antibody screening and identification, antigen typing, and in direct antiglobulin testing. In DAT, where does the sensitization step occur? - CORRECT ANSWER-in the body Direct antiglobulin tests determine what? Is IgM coating detected with this form of testing? Is IgA coating detected? What is in the control tube of this test? What results do you look for in the control and what do they mean? - CORRECT ANSWER-DAT determines *in vivo* coating of red blood cells by IgG antibody, by complement, or by both IgG and complement. IgM coating is not detected, except by complement that may have been activated. IgA coating is not detected.