AAB Molecular Diagnostics Testing Final Exams [100% PASS] /verified/

AAB Molecular Diagnostics Testing Final Exams [100% PASS] /verified/ This DNA amplification method amplifies the nucleic acid used as a probe? *Ans* Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? *Ans* DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? *Ans* Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? *Ans* Better specificity How does Ligase Chain Reaction differ from Polymerase Chain Reaction? *Ans* This method uses a thermostable ligase to join to probes which are then amplified by standard PCR cycling. Why was Ligase Chain Reaction originally developed? *Ans* To detect point mutation. What do positive controls ensure during PCR testing? *Ans* That the enzyme is active, the buffer is optimal, the primers are priming the correct sequence, and that the thermal cycler is working properly. What is a reagent blank control in PCR? *Ans* A negative control that lacks DNA to ensure that the reaction mix is not contaminated with DNA template or amplified products from a previous run. What is a negative template control in PCR? *Ans* A negative control that contains DNA that lacks this target DNA sequence. This ensures that the primers are not annealing to unintended DNA sequences. What is the purpose of the amplification control in PCR? *Ans* To distinguish between a true negative for the sample and an amplification failure (fals